Sci. Signal., 17 March 2009
Vol. 2, Issue 62, p. tr1
[DOI: 10.1126/scisignal.262tr1]
TEACHING RESOURCES
Measurement of Phosphorylated Extracellular Signal–Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology
Debbie L. Hay*
School of Biological Sciences, University of Auckland, Auckland, New Zealand.
Abstract:
Teaching the practical aspects of signal transduction to large undergraduate classes can be challenging when there is only a finite time frame in which to engage in laboratory activities. This teaching resource describes the use of bead-based ALPHAscreen technology for a class of 300 second-year biochemistry students, exposing the next generation of researchers to cutting-edge technology. Although in this case phosphorylated extracellular signal–regulated kinase 1 and 2 were measured, this technology is applicable to the measurement of many different signaling components. This resource provides a practical guide for instructors and exemplifies how such traditionally high-throughput research technologies can be used as teaching tools.
* Corresponding author. Telephone, +64-9-373-7599; fax, +64-9-373-7414; e-mail, dl.hay{at}auckland.ac.nz
Citation: D. L. Hay, Measurement of Phosphorylated Extracellular Signal–Regulated Kinase 1 and 2 in an Undergraduate Teaching Laboratory with ALPHAscreen Technology. Sci. Signal. 2, tr1 (2009).
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