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Sci. Signal., 7 April 2009
Vol. 2, Issue 65, p. ra15
[DOI: 10.1126/scisignal.2000199]

RESEARCH

Spatiotemporal Patterning During T Cell Activation Is Highly Diverse

Kentner L. Singleton1*, Kole T. Roybal1*, Yi Sun1, Guo Fu2, Nicholas R. J. Gascoigne2, Nicolai S. C. van Oers1, and Christoph Wülfing1,3{dagger}

1 Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
2 Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA.
3 Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

* These authors contributed equally to this work.

Abstract: Temporal and spatial variations in the concentrations of signaling intermediates in a living cell are important for signaling in complex networks because they modulate the probabilities that signaling intermediates will interact with each other. We have studied 30 signaling sensors, ranging from receptors to transcription factors, in the physiological activation of murine ex vivo T cells by antigen-presenting cells. Spatiotemporal patterning of these molecules was highly diverse and varied with specific T cell receptors and T cell activation conditions. The diversity and variability observed suggest that spatiotemporal patterning controls signaling interactions during T cell activation in a physiologically important and discriminating manner. In support of this, the effective clustering of a group of ligand-engaged receptors and signaling intermediates in a joint pattern consistently correlated with efficient T cell activation at the level of the whole cell.

{dagger} To whom correspondence should be addressed. E-mail: christoph.wuelfing{at}utsouthwestern.edu

Citation: K. L. Singleton, K. T. Roybal, Y. Sun, G. Fu, N. R. J. Gascoigne, N. S. C. van Oers, C. Wülfing, Spatiotemporal Patterning During T Cell Activation Is Highly Diverse. Sci. Signal. 2, ra15 (2009).

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Cytoplasmic Tail of the T Cell Receptor CD3 {epsilon} Subunit Contains a Phospholipid-Binding Motif that Regulates T Cell Functions.
L. M. DeFord-Watts, T. C. Tassin, A. M. Becker, J. J. Medeiros, J. P. Albanesi, P. E. Love, C. Wulfing, and N. S. C. van Oers (2009)
J. Immunol. 183, 1055-1064
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