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Sci. Signal., 16 June 2009
Vol. 2, Issue 75, p. pl2
[DOI: 10.1126/scisignal.275pl2]

PROTOCOLS

Using a Microfluidic Device for High-Content Analysis of Cell Signaling

Raymond Cheong, Chiaochun Joanne Wang, and Andre Levchenko*

Department of Biomedical Engineering and Whitaker Institute of Biomedical Engineering and Institute for Cell Engineering, The Johns Hopkins University, Baltimore, MD 21218, USA.

Abstract: Quantitative analysis and understanding of signaling networks require measurements of the location and activities of key proteins over time, at the level of single cells, in response to various perturbations. Microfluidic devices enable such analyses to be conducted in a high-throughput and in a highly controlled manner. We describe in detail how to design and use a microfluidic device to perform such information-rich experiments.

* Corresponding author. E-mail, alev{at}jhu.edu

Citation: R. Cheong, C. J. Wang, A. Levchenko, Using a Microfluidic Device for High-Content Analysis of Cell Signaling. Sci. Signal. 2, pl2 (2009).

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