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Sci. Signal., 24 November 2009
Vol. 2, Issue 98, p. ra77
[DOI: 10.1126/scisignal.2000466]

RESEARCH

Ca2+ Puffs Originate from Preestablished Stable Clusters of Inositol Trisphosphate Receptors

Ian F. Smith1*, Steven M. Wiltgen1, Jianwei Shuai2, and Ian Parker1,3

1 Department of Neurobiology and Behavior, University of California, Irvine, CA 92697–4550, USA.
2 Department of Physics, Xiamen University, Xiamen 361005, China.
3 Department of Physiology and Biophysics, University of California, Irvine, CA 92697–4550, USA.

Abstract: Intracellular calcium ion (Ca2+) signaling crucially depends on the clustered organization of inositol trisphosphate receptors (IP3Rs) in the endoplasmic reticulum (ER) membrane. These ligand-gated ion channels liberate Ca2+ to generate local signals known as Ca2+ puffs. We tested the hypothesis that IP3 itself elicits rapid clustering of IP3Rs by using flash photolysis of caged IP3 in conjunction with high-resolution Ca2+ imaging to monitor the activity and localization of individual IP3Rs within intact mammalian cells. Our results indicate that Ca2+ puffs arising with latencies as short as 100 to 200 ms after photorelease of IP3 already involve at least four IP3R channels, and that this number does not subsequently grow. Moreover, single active IP3Rs show limited mobility, and stochastic simulations suggest that aggregation of IP3Rs at puff sites by a diffusional trapping mechanism would require many seconds. We thus conclude that puff sites represent preestablished, stable clusters of IP3Rs and that functional IP3Rs are not readily diffusible within the ER membrane.

* To whom correspondence should be addressed. E-mail: ismith{at}uci.edu

Citation: I. F. Smith, S. M. Wiltgen, J. Shuai, I. Parker, Ca2+ Puffs Originate from Preestablished Stable Clusters of Inositol Trisphosphate Receptors. Sci. Signal. 2, ra77 (2009).

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