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Sci. STKE, 30 April 2002
Vol. 2002, Issue 130, p. pl7
[DOI: 10.1126/stke.2002.130.pl7]

PROTOCOLS

Harvesting Cells Under Anchorage-Independent Cell Transformation Conditions for Biochemical Analyses

Zigang Dong1* and Joan L. Cmarik2

1The Hormel Institute, University of Minnesota, 801 16th Avenue, NE, Austin, MN 55912, USA.
2Science Applications International Corporation, Frederick, MD 21702, USA.

Abstract: Most molecular biology and biochemical analyses use cultured cells grown in anchorage-dependent monolayer conditions. The standard oncogenic transformation assay for cell lines is usually performed in soft agar rather than in monolayers because of the higher transformation efficiency of cells in soft agar. However, cells suspended in soft agar cannot be readily recovered for studying inducible biochemical and molecular events. We developed an over-agar assay that enables us to study tumor promoter-induced cell transformation and the associated biochemical or molecular events under anchorage-independent conditions.

*Corresponding author. Telephone, 507-437-9640; fax, 507-437-9606; e-mail: zgdong{at}smig.net

Citation: Z. Dong, J. L. Cmarik, Harvesting Cells Under Anchorage-Independent Cell Transformation Conditions for Biochemical Analyses. Sci. STKE 2002, pl7 (2002).

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Science Signaling. ISSN 1937-9145 (pre-2008: Science's STKE. ISSN 1525-8882)