Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Sci. STKE, 9 November 2004
Vol. 2004, Issue 258, p. pl15
[DOI: 10.1126/stke.2582004pl15]

PROTOCOLS

Spectral Analysis of Calcium Oscillations

Per Uhlén*

Departments of Pharmacology, Cell and Molecular Physiology, Yale University School of Medicine, New Haven, CT, USA or Department of Woman and Child Health, Karolinska Institutet, Stockholm, Sweden

Abstract: Calcium (Ca2+) oscillations are universal signals exploited by cells to regulate a vast number of cellular processes. Frequency and amplitude, the key features of oscillating waves, can yield information that can be decoded by intracellular processes. Analysis and quantification of Ca2+ oscillations are crucial for understanding the general concept of Ca2+ signaling. This protocol presents a method for performing spectral analysis of Ca2+ oscillations using MATLAB software.

*Contact information. Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, CT 06520-8066, USA. Telephone, +1-203-737-1158; fax, +1-203-737-2027; e-mail, per.uhlen@yale.edu. Alternatively, Department of Woman and Child Health, Karolinska Institutet, Astrid Lindgren Children’s Hospital Q2:09, S-171 76 Stockholm, Sweden. Telephone, +46-8-51777339; fax, +46-8-51777328; e-mail, per.uhlen{at}kbh.ki.se

Citation: P. Uhlén, Spectral Analysis of Calcium Oscillations. Sci. STKE 2004, pl15 (2004).

Read the Full Text

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Non-Dioxin-like Polychlorinated Biphenyls Interfere with Neuronal Differentiation of Embryonic Neural Stem Cells.
R. Tofighi, W. N. Wan Ibrahim, P. Rebellato, P. L. Andersson, P. Uhlen, and S. Ceccatelli (2011)
Toxicol. Sci. 124, 192-201
   Abstract »    Full Text »    PDF »
Regulation of ryanodine receptor-dependent calcium signaling by polycystin-2.
G. I. Anyatonwu, M. Estrada, X. Tian, S. Somlo, and B. E. Ehrlich (2007)
PNAS 104, 6454-6459
   Abstract »    Full Text »    PDF »
Cell Type-Specific Expression of a Genetically Encoded Calcium Indicator Reveals Intrinsic Calcium Oscillations in Adult Gonadotropin-Releasing Hormone Neurons.
C. L. Jasoni, M. G. Todman, M. M. Strumia, and A. E. Herbison (2007)
J. Neurosci. 27, 860-867
   Abstract »    Full Text »    PDF »
Cytosolic inositol 1,4,5-trisphosphate dynamics during intracellular calcium oscillations in living cells.
T. Matsu-ura, T. Michikawa, T. Inoue, A. Miyawaki, M. Yoshida, and K. Mikoshiba (2006)
J. Cell Biol. 173, 755-765
   Abstract »    Full Text »    PDF »
Gain-of-function/Noonan syndrome SHP-2/Ptpn11 mutants enhance calcium oscillations and impair NFAT signaling.
P. Uhlén, P. M. Burch, C. I. Zito, M. Estrada, B. E. Ehrlich, and A. M. Bennett (2006)
PNAS 103, 2160-2165
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882