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Sci. STKE, 18 April 2006
Vol. 2006, Issue 331, p. re2
[DOI: 10.1126/stke.3312006re2]

REVIEWS

Fanciful FRET

Steven S. Vogel*, Christopher Thaler, and Srinagesh V. Koushik

National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Laboratory of Molecular Physiology, 5625 Fishers Lane, Bethesda, MD 20892, USA.

Abstract: The validity of experiments based on Förster resonance energy transfer (FRET), an imaging technique widely used to measure protein-protein interactions in living cells, critically depends on the accurate and precise measurement of FRET efficiency. The use of FRET standards to determine FRET efficiency, and a consideration of such factors as how the abundance of FRET acceptors and the stoichiometry of donors and acceptors in a molecular complex can affect measured FRET efficiency, will enhance the usefulness with which FRET experiments can be interpreted.

*Corresponding author. E-mail, stevevog{at}mail.nih.gov

Citation: S. S. Vogel, C. Thaler, S. V. Koushik, Fanciful FRET. Sci. STKE 2006, re2 (2006).

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