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Sci. STKE, 20 March 2007
[DOI: 10.1126/stke.3782007pl1]

PROTOCOLS

A Simulation Environment for Directional Sensing as a Phase Separation Process

Antonio de Candia1, Andrea Gamba2*, Fausto Cavalli3, Antonio Coniglio1, Stefano Di Talia4, Federico Bussolino5, and Guido Serini5*

1Department of Physical Sciences, Università di Napoli "Federico II," Istituto Nazionale di Fisica della Materia, and Istituto Nazionale di Fisica Nucleare, Unità di Napoli, 80126 Napoli, Italy.
2Department of Mathematics, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino, and Istituto Nazionale di Fisica Nucleare, Unità di Torino, Via Giuria 1, 10125 Torino, Italy.
3Department of Mathematics, Università degli Studi di Milano, Via Saldini 50, 20133 Milano, Italy.
4Laboratory of Mathematical Physics, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA.
5Department of Oncological Sciences and Division of Molecular Angiogenesis, IRCC, Institute for Cancer Research and Treatment, University of Torino School of Medicine, Strada Provinciale 142 Km 3.95, 10060 Candiolo (TO), Italy.

Abstract: The ability of eukaryotic cells to navigate along spatial gradients of extracellular guidance cues is crucial for embryonic development, tissue regeneration, and cancer progression. One proposed model for chemotaxis is a phosphoinositide-based phase separation process, which takes place at the plasma membrane upon chemoattractant stimulation and triggers directional motility of eukaryotic cells. Here, we make available virtual-cell software that allows the execution and spatiotemporal analysis of in silico chemotaxis experiments, in which the user can control physical and chemical parameters as well as the number and position of chemoattractant sources.

*Corresponding authors. E-mail: andrea.gamba{at}polito.it (A.G.); guido.serini{at}ircc.it (G.S.)

Citation: A. de Candia, A. Gamba, F. Cavalli, A. Coniglio, S. Di Talia, F. Bussolino, G. Serini, A Simulation Environment for Directional Sensing as a Phase Separation Process. Sci. STKE 2007, pl1 (2007).

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