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Sci. Signal., 20 July 2010
Vol. 3, Issue 131, p. lc2
[DOI: 10.1126/scisignal.3131lc2]

LETTERS

Response to Comment on "Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs"

Myriam Gorospe*, Subramanya Srikantan, Kotb Abdelmohsen, and Bernard S. Marasa{dagger}

Laboratory of Cellular and Molecular Biology, National Institute on Aging-IRP, 251 Bayview Boulevard, National Institutes of Health, Baltimore, MD 21224, USA.

{dagger} Present address: Department of Biology, The Catholic University of America, Washington, DC 20064, USA.

Abstract: Moens and colleagues express concern that our report of phosphorylation of p38–regulated/activated protein kinase (PRAK) at Ser93 in senescent cells may be incorrect as a result of their inability to detect phosphorylated PRAK with the same antibody. We provide additional characterization of the antibody that we used and show that there is a 42-kilodalton phosphoprotein detected by this antibody, but that this phosphoprotein may not be PRAK.

* Corresponding author. E-mail, myriam-gorospe{at}nih.gov; telephone, 410-558-8443; fax, 410-558-8386.

Citation: M. Gorospe, S. Srikantan, K. Abdelmohsen, B. S. Marasa, Response to Comment on "Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs". Sci. Signal. 3, lc2 (2010).

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