Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 20 July 2010
Vol. 3, Issue 131, p. lc2
[DOI: 10.1126/scisignal.3131lc2]

LETTERS

Response to Comment on "Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs"

Myriam Gorospe*, Subramanya Srikantan, Kotb Abdelmohsen, and Bernard S. Marasa{dagger}

Laboratory of Cellular and Molecular Biology, National Institute on Aging-IRP, 251 Bayview Boulevard, National Institutes of Health, Baltimore, MD 21224, USA.

{dagger} Present address: Department of Biology, The Catholic University of America, Washington, DC 20064, USA.

Abstract: Moens and colleagues express concern that our report of phosphorylation of p38–regulated/activated protein kinase (PRAK) at Ser93 in senescent cells may be incorrect as a result of their inability to detect phosphorylated PRAK with the same antibody. We provide additional characterization of the antibody that we used and show that there is a 42-kilodalton phosphoprotein detected by this antibody, but that this phosphoprotein may not be PRAK.

* Corresponding author. E-mail, myriam-gorospe{at}nih.gov; telephone, 410-558-8443; fax, 410-558-8386.

Citation: M. Gorospe, S. Srikantan, K. Abdelmohsen, B. S. Marasa, Response to Comment on "Increased MKK4 Abundance with Replicative Senescence Is Linked to the Joint Reduction of Multiple MicroRNAs". Sci. Signal. 3, lc2 (2010).

Read the Full Text



To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882