Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Sci. Signal., 28 June 2011
Vol. 4, Issue 179, p. rs6
[DOI: 10.1126/scisignal.2001588]

RESEARCH RESOURCES

Mitotic Substrates of the Kinase Aurora with Roles in Chromatin Regulation Identified Through Quantitative Phosphoproteomics of Fission Yeast

André Koch1, Karsten Krug2, Stuart Pengelley2*, Boris Macek2{dagger}, and Silke Hauf1{dagger}

1 Friedrich Miescher Laboratory of the Max Planck Society, 72076 Tuebingen, Germany.
2 Proteome Center Tuebingen, University of Tuebingen, 72076 Tuebingen, Germany.

* Present address: Bruker Daltonik GmbH, Fahrenheitstrasse 4, 28359 Bremen, Germany.

Abstract: Kinases of the Aurora family are essential for the proper execution of mitosis in eukaryotes, and Aurora inhibitors are in clinical trials as anticancer drugs. We applied site-specific quantitative phosphoproteomics in conjunction with chemical inhibition of Aurora to identify mitotic Aurora substrates in fission yeast on a proteome-wide scale. We detected 8000 phosphorylation events, of which we assigned almost 6000 to a specific residue; 220 were reduced in cells exposed to the Aurora inhibitor. After controlling for unspecific effects of the inhibitor, we classified 70 sites (on 42 proteins) as probable targets of Aurora, which enabled refinement of the consensus sequence for phosphorylation by Aurora. Several of the substrate candidates were known targets of Aurora, validating the approach, but most represented newly detected Aurora substrates. The involvement of these Aurora substrates in diverse aspects of chromatin dynamics suggests that in addition to its established role in controlling chromosome compaction and attachment to the mitotic spindle, Aurora influences other aspects of chromatin architecture and function during mitosis.

{dagger} To whom correspondence should be addressed. E-mail: boris.macek{at}uni-tuebingen.de (B.M.); silke.hauf{at}tuebingen.mpg.de (S.H.)

Citation: A. Koch, K. Krug, S. Pengelley, B. Macek, S. Hauf, Mitotic Substrates of the Kinase Aurora with Roles in Chromatin Regulation Identified Through Quantitative Phosphoproteomics of Fission Yeast. Sci. Signal. 4, rs6 (2011).

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Status of Large-scale Analysis of Post-translational Modifications by Mass Spectrometry.
J. V. Olsen and M. Mann (2013)
Mol. Cell. Proteomics 12, 3444-3452
   Abstract »    Full Text »    PDF »
Genetics and Phosphoproteomics Reveal a Protein Phosphorylation Network in the Abscisic Acid Signaling Pathway in Arabidopsis thaliana.
T. Umezawa, N. Sugiyama, F. Takahashi, J. C. Anderson, Y. Ishihama, S. C. Peck, and K. Shinozaki (2013)
Science Signaling 6, rs8
   Abstract »    Full Text »    PDF »
GIT1 Phosphorylation on Serine 46 by PKD3 Regulates Paxillin Trafficking and Cellular Protrusive Activity.
B. Huck, R. Kemkemer, M. Franz-Wachtel, B. Macek, A. Hausser, and M. A. Olayioye (2012)
J. Biol. Chem. 287, 34604-34613
   Abstract »    Full Text »    PDF »
Sos7, an Essential Component of the Conserved Schizosaccharomyces pombe Ndc80-MIND-Spc7 Complex, Identifies a New Family of Fungal Kinetochore Proteins.
V. Jakopec, B. Topolski, and U. Fleig (2012)
Mol. Cell. Biol. 32, 3308-3320
   Abstract »    Full Text »    PDF »
Global Detection of Protein Kinase D-dependent Phosphorylation Events in Nocodazole-treated Human Cells.
M. Franz-Wachtel, S. A. Eisler, K. Krug, S. Wahl, A. Carpy, A. Nordheim, K. Pfizenmaier, A. Hausser, and B. Macek (2012)
Mol. Cell. Proteomics 11, 160-170
   Abstract »    Full Text »    PDF »
Nsk1 ensures accurate chromosome segregation by promoting association of kinetochores to spindle poles during anaphase B.
G. J. Buttrick, J. C. Meadows, T. C. Lancaster, V. Vanoosthuyse, L. A. Shepperd, K.-L. Hoe, D.-U. Kim, H.-O. Park, K. G. Hardwick, and J. B. A. Millar (2011)
Mol. Biol. Cell 22, 4486-4502
   Abstract »    Full Text »    PDF »
Science Signaling Podcast: 28 June 2011.
S. Hauf, M. Winey, M. H. Jones, J. M. Keck, and A. M. VanHook (2011)
Science Signaling 4, pc12
   Abstract »    Full Text »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882