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Sci. Signal., 23 August 2011
Vol. 4, Issue 187, p. ra55
[DOI: 10.1126/scisignal.2002006]


Tumor Progression Locus 2 Mediates Signal-Induced Increases in Cytoplasmic Calcium and Cell Migration

Maria Hatziapostolou, Georgios Koukos, Christos Polytarchou, Filippos Kottakis, Oksana Serebrennikova, Athan Kuliopulos, and Philip N. Tsichlis*

Molecular Oncology Research Institute, Tufts Medical Center, Boston, MA 02111, USA.

Abstract: The mitogen-activated protein kinase kinase kinase (MAPKKK or MAP3K) tumor progression locus 2 (Tpl2) is required for the transduction of signals initiated by the thrombin-activated G protein–coupled receptor (GPCR) protease-activated receptor-1 (PAR1), which promote reorganization of the actin cytoskeleton and cell migration. Here, we show that Tpl2 is activated through Gαi2-transduced GPCR signals. Activated Tpl2 promoted the phosphorylation and activation of phospholipase C–β3 (PLCβ3); consequently, Tpl2 was required for thrombin-dependent production of inositol 1,4,5-trisphosphate (IP3), IP3-mediated cytoplasmic calcium ion (Ca2+) signals, and the activation of classical and novel members of the protein kinase C (PKC) family. A PKC-mediated feedback loop facilitated extracellular signal–regulated kinase (ERK) activation in response to Tpl2 and contributed to the coordinate regulation of the ERK and Ca2+ signaling pathways. Pharmacological and genetic studies revealed that stimulation of cell migration by Tpl2 depends on both of these pathways. Tpl2 also promoted Ca2+ signals and cell migration from sphingosine 1-phosphate–responsive GPCRs, which also couple to Gαi; from Wnt5a; and from the interleukin-1β (IL-1β) receptor, a member of the Toll–IL-1R (TIR) domain family. Our data provide new insights into the role of Tpl2 in GPCR-mediated Ca2+ signaling and cell migration.

* To whom correspondence should be addressed. E-mail: ptsichlis{at}

Citation: M. Hatziapostolou, G. Koukos, C. Polytarchou, F. Kottakis, O. Serebrennikova, A. Kuliopulos, P. N. Tsichlis, Tumor Progression Locus 2 Mediates Signal-Induced Increases in Cytoplasmic Calcium and Cell Migration. Sci. Signal. 4, ra55 (2011).

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