Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 31 January 2012
Vol. 5, Issue 209, p. ra10
[DOI: 10.1126/scisignal.2002446]


Myosin I Links PIP3 Signaling to Remodeling of the Actin Cytoskeleton in Chemotaxis

Chun-Lin Chen*, Yu Wang, Hiromi Sesaki, and Miho Iijima{dagger}

Department of Cell Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

* Present address: Department of Biological Science, National Sun Yat-Sen University, 70 Lien-Hai Road, Kaohsiung, Taiwan 80424, R.O.C.

Abstract: Class I myosins participate in various interactions between the cell membrane and the cytoskeleton. Several class I myosins preferentially bind to acidic phospholipids, such as phosphatidylserine and phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2], through a tail homology 1 (TH1) domain. Here, we show that the second messenger lipid phosphatidylinositol 3,4,5-trisphosphate (PIP3) binds to the TH1 domain of a subset of Dictyostelium class I myosins (ID, IE, and IF) and recruits them to the plasma membrane. The PIP3-regulated membrane recruitment of myosin I promoted chemotaxis and induced chemoattractant-stimulated actin polymerization. Similarly, PIP3 recruited human myosin IF to the plasma membrane upon chemotactic stimulation in a neutrophil cell line. These data suggest a mechanism through which the PIP3 signal is transmitted through myosin I to the actin cytoskeleton.

{dagger} To whom correspondence should be addressed. E-mail: miijima{at}

Citation: C.-L. Chen, Y. Wang, H. Sesaki, M. Iijima, Myosin I Links PIP3 Signaling to Remodeling of the Actin Cytoskeleton in Chemotaxis. Sci. Signal. 5, ra10 (2012).

Read the Full Text

Bleb-driven chemotaxis of Dictyostelium cells.
E. Zatulovskiy, R. Tyson, T. Bretschneider, and R. R. Kay (2014)
J. Cell Biol. 204, 1027-1044
   Abstract »    Full Text »    PDF »
Rho GTPases orient directional sensing in chemotaxis.
Y. Wang, H. Senoo, H. Sesaki, and M. Iijima (2013)
PNAS 110, E4723-E4732
   Abstract »    Full Text »    PDF »
Two distinct functions for PI3-kinases in macropinocytosis.
O. Hoeller, P. Bolourani, J. Clark, L. R. Stephens, P. T. Hawkins, O. D. Weiner, G. Weeks, and R. R. Kay (2013)
J. Cell Sci. 126, 4296-4307
   Abstract »    Full Text »    PDF »
PakB binds to the SH3 domain of Dictyostelium Abp1 and regulates its effects on cell polarity and early development.
Y. Yang, M. d. l. Roche, S. W. Crawley, Z. Li, E. Furmaniak-Kazmierczak, and G. P. Cote (2013)
Mol. Biol. Cell 24, 2216-2227
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882