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Sci. STKE, 7 May 2002
Vol. 2002, Issue 131, p. pe23
[DOI: 10.1126/stke.2002.131.pe23]

PERSPECTIVES

Sticky Caveats in an Otherwise Glowing Report: Oligomerizing Fluorescent Proteins and Their Use in Cell Biology

David A. Zacharias*

Merck Research Laboratories, 3535 General Atomics Court, MRLSDB1 Room 1400, San Diego, CA 92122, USA.

Abstract: Fluorescent proteins from sea creatures have revolutionized the study of cell biology and signal transduction in many ways. Zacharias discusses some of the technical caveats to working with these proteins when they are fused to cellular proteins to track protein localization and interactions. Special attention is paid to problems arising from oligomerization of these fluorescent proteins and how that impacts protein interactions detected by fluorescence resonance energy transfer (FRET).

*Contact information. E-mail: David_Zacharias{at}merck.com

Citation: D. A. Zacharias, Sticky Caveats in an Otherwise Glowing Report: Oligomerizing Fluorescent Proteins and Their Use in Cell Biology. Sci. STKE 2002, pe23 (2002).

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