Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Am J Physiol Heart Circ Physiol 272 (5): 2095-2106

Copyright © 1997 by the American Physiological Society.

AJP - Heart and Circulatory Physiology, Vol 272, Issue 5 2095-H2106, Copyright © 1997 by American Physiological Society


ARTICLES

Oxidants increase intracellular free Zn2+ concentration in rabbit ventricular myocytes

B. Turan, H. Fliss and M. Desilets
Department of Biophysics, Faculty of Medicine, University of Ankara, Turkey.

Oxidative stress may alter cardiac function by affecting intracellular free Zn2+ concentrations ([Zn2+]i). Rabbit ventricular myocytes loaded with fura 2 were used to fluorometrically measure resting [Zn2+]i (0.23 +/- 0.03 nM) and intracellular Ca2+ concentration ([Ca2+]i) (36 +/- 7 nM). Fluorescence quenching by the heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine was used to quantitate [Zn2+]i. The thiol-reactive oxidants hypochlorous acid (0.1 mM) and selenite (1 mM) increased [Zn2+]i to 7.7 +/- 1.7 and 6.1 +/- 1.7 nM, respectively, within 5 min. Dithiothreitol (0.5 mM), a disulfide-reducing agent, rapidly restored normal [Zn2+]i. The oxidants did not affect [Ca2+]i. However, depolarization-induced Ca2+ transients and Ca2+ currents were zinc dependent. [Zn2+]i-associated fluorescence was substantial and, if ignored, it led to overestimation of [Ca2+]i by approximately twofold before oxidant treatment and by approximately eightfold after oxidants. The results demonstrate that [Zn2+]i 1) can be greatly increased by thiol-reactive oxidants; 2) may contribute to oxidant-induced alterations of excitation-contraction coupling; and 3) has strong fura 2 fluorescence which, if overlooked, can lead to significant overestimation of [Ca2+]i.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Identifying cellular mechanisms of zinc-induced relaxation in isolated cardiomyocytes.
T. Yi, J. S. Vick, M. J. H. Vecchio, K. J. Begin, S. P. Bell, R. J. Delay, and B. M. Palmer (2013)
Am J Physiol Heart Circ Physiol 305, H706-H715
   Abstract »    Full Text »    PDF »
Thiol-oxidant monochloramine mobilizes intracellular Ca2+ in parietal cells of rabbit gastric glands.
B. M. Walsh, H. B. Naik, J. M. Dubach, M. Beshire, A. M. Wieland, and D. I. Soybel (2007)
Am J Physiol Cell Physiol 293, C1687-C1697
   Abstract »    Full Text »    PDF »
Zinc dyshomeostasis in rats with aldosteronism. Response to spironolactone.
M. Thomas, A. Vidal, S. K. Bhattacharya, R. A. Ahokas, Y. Sun, I. C. Gerling, and K. T. Weber (2007)
Am J Physiol Heart Circ Physiol 293, H2361-H2366
   Abstract »    Full Text »    PDF »
Selenium prevents diabetes-induced alterations in [Zn2+]i and metallothionein level of rat heart via restoration of cell redox cycle.
M. Ayaz and B. Turan (2006)
Am J Physiol Heart Circ Physiol 290, H1071-H1080
   Abstract »    Full Text »    PDF »
The Function of Zinc Metallothionein: A Link between Cellular Zinc and Redox State.
W. Maret (2000)
J. Nutr. 130, 1455S-1458
   Abstract »    Full Text »
Zn2+ Inhibits alpha -Ketoglutarate-stimulated Mitochondrial Respiration and the Isolated alpha -Ketoglutarate Dehydrogenase Complex.
A. M. Brown, B. S. Kristal, M. S. Effron, A. I. Shestopalov, P. A. Ullucci, K.-F. R. Sheu, J. P. Blass, and A. J. L. Cooper (2000)
J. Biol. Chem. 275, 13441-13447
   Abstract »    Full Text »    PDF »
Measurement of Intracellular Calcium.
A. Takahashi, P. Camacho, J. D. Lechleiter, and B. Herman (1999)
Physiol Rev 79, 1089-1125
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882