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Am J Physiol Heart Circ Physiol 272 (5): 2095-2106

Copyright © 1997 by the American Physiological Society.

AJP - Heart and Circulatory Physiology, Vol 272, Issue 5 2095-H2106, Copyright © 1997 by American Physiological Society


Oxidants increase intracellular free Zn2+ concentration in rabbit ventricular myocytes

B. Turan, H. Fliss and M. Desilets
Department of Biophysics, Faculty of Medicine, University of Ankara, Turkey.

Oxidative stress may alter cardiac function by affecting intracellular free Zn2+ concentrations ([Zn2+]i). Rabbit ventricular myocytes loaded with fura 2 were used to fluorometrically measure resting [Zn2+]i (0.23 +/- 0.03 nM) and intracellular Ca2+ concentration ([Ca2+]i) (36 +/- 7 nM). Fluorescence quenching by the heavy metal chelator N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine was used to quantitate [Zn2+]i. The thiol-reactive oxidants hypochlorous acid (0.1 mM) and selenite (1 mM) increased [Zn2+]i to 7.7 +/- 1.7 and 6.1 +/- 1.7 nM, respectively, within 5 min. Dithiothreitol (0.5 mM), a disulfide-reducing agent, rapidly restored normal [Zn2+]i. The oxidants did not affect [Ca2+]i. However, depolarization-induced Ca2+ transients and Ca2+ currents were zinc dependent. [Zn2+]i-associated fluorescence was substantial and, if ignored, it led to overestimation of [Ca2+]i by approximately twofold before oxidant treatment and by approximately eightfold after oxidants. The results demonstrate that [Zn2+]i 1) can be greatly increased by thiol-reactive oxidants; 2) may contribute to oxidant-induced alterations of excitation-contraction coupling; and 3) has strong fura 2 fluorescence which, if overlooked, can lead to significant overestimation of [Ca2+]i.

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Thiol-oxidant monochloramine mobilizes intracellular Ca2+ in parietal cells of rabbit gastric glands.
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Zinc dyshomeostasis in rats with aldosteronism. Response to spironolactone.
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The Function of Zinc Metallothionein: A Link between Cellular Zinc and Redox State.
W. Maret (2000)
J. Nutr. 130, 1455S-1458
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Zn2+ Inhibits alpha -Ketoglutarate-stimulated Mitochondrial Respiration and the Isolated alpha -Ketoglutarate Dehydrogenase Complex.
A. M. Brown, B. S. Kristal, M. S. Effron, A. I. Shestopalov, P. A. Ullucci, K.-F. R. Sheu, J. P. Blass, and A. J. L. Cooper (2000)
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Measurement of Intracellular Calcium.
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