Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Development 130 (23): 5779-5789

Caspase-independent cell engulfment mirrors cell death pattern in Drosophila embryos

Jaime Mergliano, and Jonathan S. Minden*

Department of Biological Sciences and Science and Technology Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA

* Author of correspondence (e-mail: minden{at}cmu.edu)

Accepted for publication 20 August 2003.

Abstract: Programmed cell death plays an essential role during Drosophila embryonic development. A stereotypic series of cellular changes occur during apoptosis, most of which are initiated by a caspase cascade that is triggered by a trio of proteins, RPR, HID and GRIM. The final step in apoptosis is engulfment of the cell corpse. To monitor cell engulfment in vivo, we developed a fluorogenic ß-galactosidase substrate that is cleaved by an endogenous, lysosomal ß-galactosidase activity. The pattern of cell engulfment in wild-type embryos correlated well with the known pattern of apoptosis. Surprisingly, the pattern of cell engulfment persisted in apoptosis-deficient embryos. We provide evidence for a caspase-independent engulfment process that affects the majority of cells expected to die in developing Drosophila embryos.

Key Words: Drosophila • Embryo • Engulfment • Apoptosis • H99 • p35 • Phagocytosis


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The columnar gene vnd is required for tritocerebral neuromere formation during embryonic brain development of Drosophila.
S. G. Sprecher, R. Urbach, G. M. Technau, F. M. Rijli, H. Reichert, and F. Hirth (2006)
Development 133, 4331-4339
   Abstract »    Full Text »    PDF »
Cellular mechanisms of dendrite pruning in Drosophila: insights from in vivo time-lapse of remodeling dendritic arborizing sensory neurons.
D. W. Williams and J. W. Truman (2005)
Development 132, 3631-3642
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882