Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Development 132 (2): 247-256

Formation of the retinotectal projection requires Esrom, an ortholog of PAM (protein associated with Myc)

Jasmine D'Souza1, Michael Hendricks1, Sylvie Le Guyader1, Sivan Subburaju1, Barbara Grunewald2, Klaus Scholich3, and Suresh Jesuthasan1,*

1 Developmental Neurobiology Group, Temasek Life Sciences Laboratory, 1 Research Link, Singapore 117604, Rep. of Singapore
2 Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany
3 Pharmazentrum Frankfurt, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt, 60590 Frankfurt, Germany

* Author for correspondence (e-mail: suresh{at}tll.org.sg)

Accepted for publication 29 October 2004.

Abstract: Visual system development is dependent on correct interpretation of cues that direct growth cone migration and axon branching. Mutations in the zebrafish esrom gene disrupt bundling and targeting of retinal axons, and also cause ectopic arborization. By positional cloning, we establish that esrom encodes a very large protein orthologous to PAM (protein associated with Myc)/Highwire/RPM-1. Unlike motoneurons in Drosophila highwire mutants, retinal axons in esrom mutants do not arborize excessively, indicating that Esrom has different functions in the vertebrate visual system. We show here that Esrom has E3 ligase activity and modulates the amount of phosphorylated Tuberin, a tumor suppressor, in growth cones. These data identify a mediator of signal transduction in retinal growth cones, which is required for topographic map formation.

Key Words: Retinotectal projection • Topographic mapping • Fasciculation • Axon branching • Visual system • Zebrafish

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Molecular Control of Axon Branching.
P. M. Bilimoria and A. Bonni (2013)
Neuroscientist 19, 16-24
   Abstract »    Full Text »    PDF »
The E3 Ubiquitin Ligase Protein Associated with Myc (Pam) Regulates Mammalian/Mechanistic Target of Rapamycin Complex 1 (mTORC1) Signaling in Vivo through N- and C-terminal Domains.
S. Han, S. Kim, S. Bahl, L. Li, C. F. Burande, N. Smith, M. James, R. L. Beauchamp, P. Bhide, A. DiAntonio, et al. (2012)
J. Biol. Chem. 287, 30063-30072
   Abstract »    Full Text »    PDF »
PPM-1, a PP2C{alpha}/{beta} phosphatase, Regulates Axon Termination and Synapse Formation in Caenorhabditis elegans.
E. D. Tulgren, S. T. Baker, L. Rapp, A. M. Gurney, and B. Grill (2011)
Genetics 189, 1297-1307
   Abstract »    Full Text »    PDF »
Highwire Regulates Guidance of Sister Axons in the Drosophila Mushroom Body.
J. E. Shin and A. DiAntonio (2011)
J. Neurosci. 31, 17689-17700
   Abstract »    Full Text »    PDF »
The Ubiquitin Ligase MYCBP2 Regulates Transient Receptor Potential Vanilloid Receptor 1 (TRPV1) Internalization through Inhibition of p38 MAPK Signaling.
S. Holland, O. Coste, D. D. Zhang, S. C. Pierre, G. Geisslinger, and K. Scholich (2011)
J. Biol. Chem. 286, 3671-3680
   Abstract »    Full Text »    PDF »
A Ubiquitin E2 Variant Protein Acts in Axon Termination and Synaptogenesis in Caenorhabditis elegans.
G. Trujillo, K. Nakata, D. Yan, I. N. Maruyama, and Y. Jin (2010)
Genetics 186, 135-145
   Abstract »    Full Text »    PDF »
Fbxo45 Forms a Novel Ubiquitin Ligase Complex and Is Required for Neuronal Development.
T. Saiga, T. Fukuda, M. Matsumoto, H. Tada, H. J. Okano, H. Okano, and K. I. Nakayama (2009)
Mol. Cell. Biol. 29, 3529-3543
   Abstract »    Full Text »    PDF »
PHR Regulates Growth Cone Pausing at Intermediate Targets through Microtubule Disassembly.
M. Hendricks and S. Jesuthasan (2009)
J. Neurosci. 29, 6593-6598
   Abstract »    Full Text »    PDF »
Toponomics Analysis of Functional Interactions of the Ubiquitin Ligase PAM (Protein Associated with Myc) during Spinal Nociceptive Processing.
S. Pierre, C. Maeurer, O. Coste, W. Becker, A. Schmidtko, S. Holland, C. Wittpoth, G. Geisslinger, and K. Scholich (2008)
Mol. Cell. Proteomics 7, 2475-2485
   Abstract »    Full Text »    PDF »
A Mechanism Distinct from Highwire for the Drosophila Ubiquitin Conjugase Bendless in Synaptic Growth and Maturation.
S. B. Uthaman, T. A. Godenschwege, and R. K. Murphey (2008)
J. Neurosci. 28, 8615-8623
   Abstract »    Full Text »    PDF »
RPM-1, a Caenorhabditis elegans Protein That Functions in Presynaptic Differentiation, Negatively Regulates Axon Outgrowth by Controlling SAX-3/robo and UNC-5/UNC5 Activity.
H. Li, G. Kulkarni, and W. G. Wadsworth (2008)
J. Neurosci. 28, 3595-3603
   Abstract »    Full Text »    PDF »
The requirement for Phr1 in CNS axon tract formation reveals the corticostriatal boundary as a choice point for cortical axons.
A. J. Bloom, B. R. Miller, J. R. Sanes, and A. DiAntonio (2007)
Genes & Dev. 21, 2593-2606
   Abstract »    Full Text »    PDF »
Highwire Function at the Drosophila Neuromuscular Junction: Spatial, Structural, and Temporal Requirements.
C. Wu, Y. P. Wairkar, C. A. Collins, and A. DiAntonio (2005)
J. Neurosci. 25, 9557-9566
   Abstract »    Full Text »    PDF »
A GFP-based genetic screen reveals mutations that disrupt the architecture of the zebrafish retinotectal projection.
T. Xiao, T. Roeser, W. Staub, and H. Baier (2005)
Development 132, 2955-2967
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882