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Development 133 (15): 2827-2833

A translation-independent role of oskar RNA in early Drosophila oogenesis

Andreas Jenny1,*,{dagger}, Olivier Hachet1,{dagger},{ddagger}, Péter Závorszky1,2, Anna Cyrklaff1, Matthew D. J. Weston3,§, Daniel St Johnston3, Miklós Erdélyi2, and Anne Ephrussi1

1 European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany.
2 Biological Research Center of the Hungarian Academy of Sciences, Institute of Genetics, H-6701 Szeged, POB 521, Temesvari krt. 62, Hungary.
3 The Wellcome Trust/Cancer Research UK Gurdon Institute & the Department of Genetics, University of Cambridge, Tennis Court Rd, Cambridge CB2 1QN, UK.

Authors for correspondence (e-mail: ephrussi{at}embl.de; erdelyim{at}brc.hu)

Accepted for publication 23 May 2006.

Abstract: The Drosophila maternal effect gene oskar encodes the posterior determinant responsible for the formation of the posterior pole plasm in the egg, and thus of the abdomen and germline of the future fly. Previously identified oskar mutants give rise to offspring that lack both abdominal segments and a germline, thus defining the `posterior group phenotype'. Common to these classical oskar alleles is that they all produce significant amounts of oskar mRNA. By contrast, two new oskar mutants in which oskar RNA levels are strongly reduced or undetectable are sterile, because of an early arrest of oogenesis. This egg-less phenotype is complemented by oskar nonsense mutant alleles, as well as by oskar transgenes, the protein-coding capacities of which have been annulled. Moreover, we show that expression of the oskar 3' untranslated region (3'UTR) is sufficient to rescue the egg-less defect of the RNA null mutant. Our analysis thus reveals an unexpected role for oskar RNA during early oogenesis, independent of Oskar protein. These findings indicate that oskar RNA acts as a scaffold or regulatory RNA essential for development of the oocyte.

Key Words: oskar • Non-coding RNA • Polarity • Oogenesis • Drosophila


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