Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Development 135 (4): 627-636

Logic of Wg and Dpp induction of distal and medial fates in the Drosophila leg

Carlos Estella, and Richard S. Mann*

Department of Biochemistry and Molecular Biophysics, Columbia University, 701 West 168th Street, HHSC 1104, New York, NY 10032, USA.

* Author for correspondence (e-mail: rsm10{at}columbia.edu)

Accepted for publication 29 November 2007.

Abstract: Drosophila leg development requires the cooperation of two secreted signals, Decapentaplegic (Dpp) and Wingless (Wg), to form the proximodistal (PD) axis. Wg and Dpp are also required to pattern the dorsoventral (DV) axis of the leg. Here, we show that Distalless (Dll) and dachshund (dac), genes expressed at different positions along the PD axis, are activated by Wg signaling and repressed by Brinker (Brk), a transcriptional repressor in the Dpp pathway. The levels of both Brk and Wg determine which of these PD genes is activated. Surprisingly, Brk does not play a role in DV axis specification in the leg, suggesting that Dpp uses two distinct mechanisms for generating the PD and DV axes. Based on these results, we present a model for how Dpp and Wg, which are present as dorsal and ventral gradients, respectively, induce nearly circular domains of gene expression along the PD axis.

Key Words: Brinker • Decapentaplegic • Wingless • Leg development • Morphogens • Proximodistal axis


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The miR-310/13 cluster antagonizes {beta}-catenin function in the regulation of germ and somatic cell differentiation in the Drosophila testis.
R. Pancratov, F. Peng, P. Smibert, J.-S. Yang, E. R. Olson, C. Guha-Gilford, A. J. Kapoor, F.-X. Liang, E. C. Lai, M. S. Flaherty, et al. (2013)
Development 140, 2904-2916
   Abstract »    Full Text »    PDF »
Transcription factor choice in the Hippo signaling pathway: homothorax and yorkie regulation of the microRNA bantam in the progenitor domain of the Drosophila eye imaginal disc.
H. W. Peng, M. Slattery, and R. S. Mann (2009)
Genes & Dev. 23, 2307-2319
   Abstract »    Full Text »    PDF »
The Tbx20 homologs midline and H15 specify ventral fate in the Drosophila melanogaster leg.
P. C. Svendsen, A. Formaz-Preston, S. M. Leal, and W. J. Brook (2009)
Development 136, 2689-2693
   Abstract »    Full Text »    PDF »
Lineage and Birth Date Specify Motor Neuron Targeting and Dendritic Architecture in Adult Drosophila.
M. Baek and R. S. Mann (2009)
J. Neurosci. 29, 6904-6916
   Abstract »    Full Text »    PDF »
The origins of the Drosophila leg revealed by the cis-regulatory architecture of the Distalless gene.
D. J. McKay, C. Estella, and R. S. Mann (2009)
Development 136, 61-71
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882