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Development 138 (9): 1697-1703


Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling

Yan Yan, Natalie Denef, Charm Tang, and Trudi Schüpbach*

Howard Hughes Medical Institute, Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA

* Author for correspondence (schupbac{at}

Accepted for publication 24 January 2011.

Abstract: In a genetic screen we isolated mutations in CG10260, which encodes a phosphatidylinositol 4-kinase (PI4KIIIalpha), and found that PI4KIIIalpha is required for Hippo signaling in Drosophila ovarian follicle cells. PI4KIIIalpha mutations in the posterior follicle cells lead to oocyte polarization defects similar to those caused by mutations in the Hippo signaling pathway. PI4KIIIalpha mutations also cause misexpression of well-established Hippo signaling targets. The Merlin-Expanded-Kibra complex is required at the apical membrane for Hippo activity. In PI4KIIIalpha mutant follicle cells, Merlin fails to localize to the apical domain. Our analysis of PI4KIIIalpha mutants provides a new link in Hippo signal transduction from the cell membrane to its core kinase cascade.

Key Words: Drosophila • Hippo signaling • Merlin • PI4kinase • Oocyte polarity

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