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Vol. 16, No. 18, pp. 2379-2389, September 15, 2002
RESEARCH PAPER
Negative regulation of STAT92E by an N-terminally truncated STAT protein derived from an alternative promoter site
Melissa A.
Henriksen,1
Aurel
Betz,1
Marc V.
Fuccillo, and
James E.
Darnell Jr.2
Laboratory of Molecular Cell Biology, The Rockefeller University,
New York, New York 10021, USA
Previously unrecognized mRNAs originating from a dual promoter at
the stat92E locus are described. One of these encodes atruncated protein, NSTAT92E, that lacks the N-terminal 133 aminoacids. Antibodies detect both the full-length and truncated moleculesearly in embryogenesis (1-5 h), and mRNA detection by specificRT-PCR
reactions accords with the protein distribution. Giventhat the N
termini of mammalian STATs are known to have positivefunctions in
transcriptional activation, we explored the roleof NSTAT92E early
in embryogenesis. By increasing the NSTAT92E-to-STAT92Eratio in
overexpression and RNAi experiments, we observe phenotypescompatible
with suppression of wild-type STAT92E activity. Wetherefore conclude
that the short form of STAT92E is a naturallyoccurring
dominant-negative product that can be added to the growinglist of
negative regulators of STAT activity.
[Key Words:Drosophila melanogaster; STAT92E; alternative promoters; differential splicing]
Not4 enhances JAK/STAT pathway-dependent gene expression in Drosophila and in human cells.
J. Gronholm, M. Kaustio, H. Myllymaki, J. Kallio, J. Saarikettu, J. Kronhamn, S. Valanne, O. Silvennoinen, and M. Ramet (2012)
FASEB J
26, 1239-1250
|Abstract »|Full Text »|PDF »
Cytokine signaling through the JAK/STAT pathway is required for long-term memory in Drosophila.
T. Copf, V. Goguel, A. Lampin-Saint-Amaux, N. Scaplehorn, and T. Preat (2011)
PNAS
108, 8059-8064
|Abstract »|Full Text »|PDF »
STAT92E is a positive regulator of Drosophila inhibitor of apoptosis 1 (DIAP/1) and protects against radiation-induced apoptosis.
A. Betz, H. D. Ryoo, H. Steller, and J. E. Darnell Jr (2008)
PNAS
105, 13805-13810
|Abstract »|Full Text »|PDF »
C. elegans STAT: evolution of a regulatory switch.
Wnt Activation and Alternative Promoter Repression of LEF1 in Colon Cancer.
T. W.-H. Li, J.-H. T. Ting, N. N. Yokoyama, A. Bernstein, M. van de Wetering, and M. L. Waterman (2006)
Mol. Cell. Biol.
26, 5284-5299
|Abstract »|Full Text »|PDF »
JAK/STAT signalling in Drosophila: insights into conserved regulatory and cellular functions..
Essential Role of STAT3 in Postnatal Survival and Growth Revealed by Mice Lacking STAT3 Serine 727 Phosphorylation.
Y. Shen, K. Schlessinger, X. Zhu, E. Meffre, F. Quimby, D. E. Levy, and J. E. Darnell Jr. (2004)
Mol. Cell. Biol.
24, 407-419
|Abstract »|Full Text »|PDF »
Impact of Alternative Initiation, Splicing, and Termination on the Diversity of the mRNA Transcripts Encoded by the Mouse Transcriptome.
M. Zavolan, S. Kondo, C. Schonbach, J. Adachi, D. A. Hume, RIKEN GER Group, GSL Members, Y. Hayashizaki, and T. Gaasterland (2003)
Genome Res.
13, 1290-1300
|Abstract »|Full Text »|PDF »
Isolation of the Novel Human Guanine Nucleotide Exchange Factor Src Homology 3 Domain-Containing Guanine Nucleotide Exchange Factor (SGEF) and of C-Terminal SGEF, an N-Terminally Truncated Form of SGEF, the Expression of Which Is Regulated by Androgen in Prostate Cancer Cells.
H. Qi, A. Fournier, J. Grenier, C. Fillion, Y. Labrie, and C. Labrie (2003)
Endocrinology
144, 1742-1752
|Abstract »|Full Text »|PDF »
NSTAT92E, that lacks the N-terminal 133 amino acids. Antibodies detect both the full-length and truncated molecules early in embryogenesis (1-5 h), and mRNA detection by specific RT-PCR
reactions accords with the protein distribution. Given that the N
termini of mammalian STATs are known to have positive functions in
transcriptional activation, we explored the role of 
