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Generation of Ski-knockdown mice by expressing a long double-strand RNA from an RNA polymerase II promoter
Toshie Shinagawa, and
Laboratory of Molecular Genetics, RIKEN Tsukuba Institute, Tsukuba,
Ibaraki 305-0074, Japan
We have developed a new vector, named pDECAP, to express longdouble-strand
RNA (ds-RNA) from an RNA polymerase II (Pol II)promoter. Because the
transcripts from pDECAP lack both the5'-cap structure and the
3'-poly(A) tail that facilitate ds-RNAexport to the cytoplasm, long
ds-RNA from pDECAP does not inducethe interferon response. Transgenic mice
embryos expressinglong ds-RNA for the transcriptional corepressor Ski from
thisvector exhibited phenotypes that were remarkably similar tothose of
Ski-deficient embryos, including defects of neuraltube closure and
eye formation. Thus, this vector providesa new tool to efficiently generate
tissue-specific knockdownmice for studying gene function in whole animal
Key Words: RNA interference double-strand RNA transgenic mice pol II promoter Ski corepressor
Received for publication January 8, 2003.
Accepted for publication April 9, 2003.