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Genes & Dev. 20 (16): 2202-2207

Copyright © 2006 by Cold Spring Harbor Laboratory Press.

RESEARCH COMMUNICATION

Extensive post-transcriptional regulation of microRNAs and its implications for cancer

J. Michael Thomson1, Martin Newman1, Joel S. Parker4, Elizabeth M. Morin-Kensicki1, Tricia Wright2, and Scott M. Hammond1,3,5

1 Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA;
2 Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA;
3 Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, North Carolina 27599, USA;
4 Constella Group, Durham, North Carolina 27713, USA

Abstract: MicroRNAs (miRNAs) are short, noncoding RNAs that post-transcriptionally regulate gene expression. While hundreds of mammalian miRNA genes have been identified, little is known about the pathways that regulate the production of active miRNA species. Here we show that a large fraction of miRNA genes are regulated post-transcriptionally. During early mouse development, many miRNA primary transcripts, including the Let-7 family, are present at high levels but are not processed by the enzyme Drosha. An analysis of gene expression in primary tumors indicates that the widespread down-regulation of miRNAs observed in cancer is due to a failure at the Drosha processing step. These data uncover a novel regulatory step in miRNA function and provide a mechanism for miRNA down-regulation in cancer.

Key Words: miRNA • microRNA • let-7 • RISC • Drosha • cancer

Received for publication April 27, 2006. Accepted for publication June 14, 2006.

5 Corresponding author.

E-MAIL hammond{at}med.unc.edu; FAX (919) 966-1856.

Supplemental material is available at http://www.genesdev.org.

Article published online ahead of print. Article and publication date are online at http://www.genesdev.org/cgi/doi/10.1101/gad.1444406.

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. M. Pare, N. Tahbaz, J. Lopez-Orozco, P. LaPointe, P. Lasko, and T. C. Hobman (2009)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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N. VALERI, C. M. CROCE, and M. FABBRI (2009)
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   Abstract »    Full Text »    PDF »
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S. Sakamoto, K. Aoki, T. Higuchi, H. Todaka, K. Morisawa, N. Tamaki, E. Hatano, A. Fukushima, T. Taniguchi, and Y. Agata (2009)
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   Abstract »    Full Text »    PDF »
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R. Triboulet, H.-M. Chang, R. J. LaPierre, and R. I. Gregory (2009)
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   Abstract »    Full Text »    PDF »
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N. S. Wickramasinghe, T. T. Manavalan, S. M. Dougherty, K. A. Riggs, Y. Li, and C. M. Klinge (2009)
Nucleic Acids Res. 37, 2584-2595
   Abstract »    Full Text »    PDF »
Cell-cell contact globally activates microRNA biogenesis.
H.-W. Hwang, E. A. Wentzel, and J. T. Mendell (2009)
PNAS 106, 7016-7021
   Abstract »    Full Text »    PDF »
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D. Lebanony, H. Benjamin, S. Gilad, M. Ezagouri, A. Dov, K. Ashkenazi, N. Gefen, S. Izraeli, G. Rechavi, H. Pass, et al. (2009)
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   Abstract »    Full Text »    PDF »
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S. Rane, M. He, D. Sayed, H. Vashistha, A. Malhotra, J. Sadoshima, D. E. Vatner, S. F. Vatner, and M. Abdellatif (2009)
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   Abstract »    Full Text »    PDF »
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H. M. Kurth and K. Mochizuki (2009)
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   Abstract »    Full Text »    PDF »
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T. Uziel, F. V. Karginov, S. Xie, J. S. Parker, Y.-D. Wang, A. Gajjar, L. He, D. Ellison, R. J. Gilbertson, G. Hannon, et al. (2009)
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   Abstract »    Full Text »    PDF »
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T. Katoh, Y. Sakaguchi, K. Miyauchi, T. Suzuki, S.-i. Kashiwabara, T. Baba, and T. Suzuki (2009)
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   Abstract »    Full Text »    PDF »
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N. Yang, S. Kaur, S. Volinia, J. Greshock, H. Lassus, K. Hasegawa, S. Liang, A. Leminen, S. Deng, L. Smith, et al. (2008)
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   Abstract »    Full Text »    PDF »
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Y. Horikawa, C. G. Wood, H. Yang, H. Zhao, Y. Ye, J. Gu, J. Lin, T. Habuchi, and X. Wu (2008)
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   Abstract »    Full Text »    PDF »
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N. J. Martinez, M. C. Ow, J. S. Reece-Hoyes, M. I. Barrasa, V. R. Ambros, and A. J.M. Walhout (2008)
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   Abstract »    Full Text »    PDF »
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S. Tokumaru, M. Suzuki, H. Yamada, M. Nagino, and T. Takahashi (2008)
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   Abstract »    Full Text »    PDF »
Primary microRNA transcript retention at sites of transcription leads to enhanced microRNA production.
J. M. Pawlicki and J. A. Steitz (2008)
J. Cell Biol. 182, 61-76
   Abstract »    Full Text »    PDF »
An Insect Virus-Encoded MicroRNA Regulates Viral Replication.
M. Hussain, R. J. Taft, and S. Asgari (2008)
J. Virol. 82, 9164-9170
   Abstract »    Full Text »    PDF »
The FLYWCH transcription factors FLH-1, FLH-2, and FLH-3 repress embryonic expression of microRNA genes in C. elegans.
M. C. Ow, N. J. Martinez, P. H. Olsen, H. S. Silverman, M. I. Barrasa, B. Conradt, A. J.M. Walhout, and V. Ambros (2008)
Genes & Dev. 22, 2520-2534
   Abstract »    Full Text »    PDF »
Frequency and fate of microRNA editing in human brain.
Y. Kawahara, M. Megraw, E. Kreider, H. Iizasa, L. Valente, A. G. Hatzigeorgiou, and K. Nishikura (2008)
Nucleic Acids Res. 36, 5270-5280
   Abstract »    Full Text »    PDF »
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C. Urbich, A. Kuehbacher, and S. Dimmeler (2008)
Cardiovasc Res 79, 581-588
   Abstract »    Full Text »    PDF »
Lin-28 interaction with the Let-7 precursor loop mediates regulated microRNA processing.
M. A. Newman, J. M. Thomson, and S. M. Hammond (2008)
RNA 14, 1539-1549
   Abstract »    Full Text »    PDF »
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S. Ambs, R. L. Prueitt, M. Yi, R. S. Hudson, T. M. Howe, F. Petrocca, T. A. Wallace, C.-G. Liu, S. Volinia, G. A. Calin, et al. (2008)
Cancer Res. 68, 6162-6170
   Abstract »    Full Text »    PDF »
Diagnostic and Prognostic MicroRNAs in Stage II Colon Cancer.
T. Schepeler, J. T. Reinert, M. S. Ostenfeld, L. L. Christensen, A. N. Silahtaroglu, L. Dyrskjot, C. Wiuf, F. J. Sorensen, M. Kruhoffer, S. Laurberg, et al. (2008)
Cancer Res. 68, 6416-6424
   Abstract »    Full Text »    PDF »
Determinants of MicroRNA Processing Inhibition by the Developmentally Regulated RNA-binding Protein Lin28.
E. Piskounova, S. R. Viswanathan, M. Janas, R. J. LaPierre, G. Q. Daley, P. Sliz, and R. I. Gregory (2008)
J. Biol. Chem. 283, 21310-21314
   Abstract »    Full Text »    PDF »
Inducible expression of microRNA-194 is regulated by HNF-1{alpha} during intestinal epithelial cell differentiation.
K. Hino, K. Tsuchiya, T. Fukao, K. Kiga, R. Okamoto, T. Kanai, and M. Watanabe (2008)
RNA 14, 1433-1442
   Abstract »    Full Text »    PDF »
Genomic and epigenetic alterations deregulate microRNA expression in human epithelial ovarian cancer.
L. Zhang, S. Volinia, T. Bonome, G. A. Calin, J. Greshock, N. Yang, C.-G. Liu, A. Giannakakis, P. Alexiou, K. Hasegawa, et al. (2008)
PNAS 105, 7004-7009
   Abstract »    Full Text »    PDF »
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Y. Lu, T. Okubo, E. Rawlins, and B. L. M. Hogan (2008)
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   Abstract »    Full Text »    PDF »
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A. G. Bang and M. K. Carpenter (2008)
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   Abstract »    Full Text »    PDF »
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S. R. Viswanathan, G. Q. Daley, and R. I. Gregory (2008)
Science 320, 97-100
   Abstract »    Full Text »    PDF »
Exploration of Tumor-Suppressive MicroRNAs Silenced by DNA Hypermethylation in Oral Cancer.
K.-i. Kozaki, I. Imoto, S. Mogi, K. Omura, and J. Inazawa (2008)
Cancer Res. 68, 2094-2105
   Abstract »    Full Text »    PDF »
Gene Regulation by Transcription Factors and MicroRNAs.
O. Hobert (2008)
Science 319, 1785-1786
   Abstract »    Full Text »    PDF »
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L. T. Bemis, R. Chen, C. M. Amato, E. H. Classen, S. E. Robinson, D. G. Coffey, P. F. Erickson, Y. G. Shellman, and W. A. Robinson (2008)
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   Abstract »    Full Text »    PDF »
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J. Y. Lee, S. Kim, D. W. Hwang, J. M. Jeong, J.-K. Chung, M. C. Lee, and D. S. Lee (2008)
J. Nucl. Med. 49, 285-294
   Abstract »    Full Text »    PDF »
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E. J. Lee, M. Baek, Y. Gusev, D. J. Brackett, G. J. Nuovo, and T. D. Schmittgen (2008)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
MicroRNAs as Potential Agents to Alter Resistance to Cytotoxic Anticancer Therapy.
J. B. Weidhaas, I. Babar, S. M. Nallur, P. Trang, S. Roush, M. Boehm, E. Gillespie, and F. J. Slack (2007)
Cancer Res. 67, 11111-11116
   Abstract »    Full Text »    PDF »
A simple array platform for microRNA analysis and its application in mouse tissues.
X. Tang, J. Gal, X. Zhuang, W. Wang, H. Zhu, and G. Tang (2007)
RNA 13, 1803-1822
   Abstract »    Full Text »    PDF »
MicroRNA-34b and MicroRNA-34c Are Targets of p53 and Cooperate in Control of Cell Proliferation and Adhesion-Independent Growth.
D. C. Corney, A. Flesken-Nikitin, A. K. Godwin, W. Wang, and A. Yu. Nikitin (2007)
Cancer Res. 67, 8433-8438
   Abstract »    Full Text »    PDF »
GeneHub-GEPIS: digital expression profiling for normal and cancer tissues based on an integrated gene database.
Y. Zhang, S.-M. Luoh, L. S. Hon, R. Baertsch, W. I. Wood, and Z. Zhang (2007)
Nucleic Acids Res. 35, W152-W158
   Abstract »    Full Text »    PDF »
MicroRNA Expression Profiling in Prostate Cancer.
K. P. Porkka, M. J. Pfeiffer, K. K. Waltering, R. L. Vessella, T. L.J. Tammela, and T. Visakorpi (2007)
Cancer Res. 67, 6130-6135
   Abstract »    Full Text »    PDF »
Oncogenic All1 fusion proteins target Drosha-mediated microRNA processing.
T. Nakamura, E. Canaani, and C. M. Croce (2007)
PNAS 104, 10980-10985
   Abstract »    Full Text »    PDF »
Oncogenic Potential of the miR-106-363 Cluster and Its Implication in Human T-Cell Leukemia.
S. Landais, S. Landry, P. Legault, and E. Rassart (2007)
Cancer Res. 67, 5699-5707
   Abstract »    Full Text »    PDF »

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