Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

J. Biol. Chem. 277 (48): 46493-46503

© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

Distinct Signaling Pathways Are Activated in Response to Mechanical Stress Applied Axially and Transversely to Skeletal Muscle Fibers*

Ashok Kumar, Imran Chaudhry, Michael B. Reid, and Aladin M. BoriekDagger

From the Department of Medicine, Baylor College of Medicine, Houston, Texas 77030

In the diaphragm muscle we tested the hypothesis that MAP kinase signaling pathways are activated by mechanical stress and such signaling pathways are dependent on the direction in which mechanical stress is applied. Although equal magnitudes of mechanical stress were applied axially and transversely a greater level of activation of ERK1/2, p38, Raf-1, p90 RSK, Elk-1, and the DNA binding activity of AP-1 transcription factor was produced when the muscle was stretched transversely than when stretched axially. A significant up-regulation in protein tyrosine phosphorylation was observed in axially or transversely loaded diaphragm muscles and the activation of ERK1/2 was completely inhibited by genistein (protein-tyrosine kinase inhibitor). Pretreatment of muscles with wortmannin (phosphoinositide 3-kinase inhibitor), TMB-8 (antagonist of intracellular calcium release), GF109203X (PKC inhibitor), or PD98059 (MEK1/2 inhibitor) blocked the activation of ERK1/2 kinases in response to axial but not to transverse loading. On the other hand, pretreatment of muscles with protein kinase A inhibitors H-7 and KT5720 completely suppressed the activation of ERK1/2 in response to transverse loading only. Taken together with the alterations of MAP kinases and the findings of elevations of downstream transcription targets, our data are consistent with two distinct MAP kinase signal transduction pathways in response to mechanical stress.

* This work was supported by National Institutes of Health Grants RO1-63134 and RO1-46230 (to A. B.) and HL45721 (to M. B. R.).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger To whom correspondence should be addressed: Pulmonary and Critical Care, Suite 520B, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030. Fax: 713-798-3619; E-mail: boriek@bcm.tmc.edu.

Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Sustained postexercise increases in AS160 Thr642 and Ser588 phosphorylation in skeletal muscle without sustained increases in kinase phosphorylation.
G. G. Schweitzer, E. B. Arias, and G. D. Cartee (2012)
J Appl Physiol 113, 1852-1861
   Abstract »    Full Text »    PDF »
Massage Therapy Attenuates Inflammatory Signaling After Exercise-Induced Muscle Damage.
J. D. Crane, D. I. Ogborn, C. Cupido, S. Melov, A. Hubbard, J. M. Bourgeois, and M. A. Tarnopolsky (2012)
Science Translational Medicine 4, 119ra13
   Abstract »    Full Text »    PDF »
MAPKs and NF-{kappa}B differentially regulate cytokine expression in the diaphragm in response to resistive breathing: the role of oxidative stress.
I. Sigala, P. Zacharatos, D. Toumpanakis, T. Michailidou, O. Noussia, S. Theocharis, C. Roussos, A. Papapetropoulos, and T. Vassilakopoulos (2011)
Am J Physiol Regulatory Integrative Comp Physiol 300, R1152-R1162
   Abstract »    Full Text »    PDF »
Induction of Sirt1 by Mechanical Stretch of Skeletal Muscle through the Early Response Factor EGR1 Triggers an Antioxidative Response.
P. S. Pardo, J. S. Mohamed, M. A. Lopez, and A. M. Boriek (2011)
J. Biol. Chem. 286, 2559-2566
   Abstract »    Full Text »    PDF »
Anisotropic regulation of Ankrd2 gene expression in skeletal muscle by mechanical stretch.
J. S. Mohamed, M. A. Lopez, G. A. Cox, and A. M. Boriek (2010)
FASEB J 24, 3330-3340
   Abstract »    Full Text »    PDF »
Stretch augments TGF-{beta}1 expression through RhoA/ROCK1/2, PTK, and PI3K in airway smooth muscle cells.
J. S. Mohamed and A. M. Boriek (2010)
Am J Physiol Lung Cell Mol Physiol 299, L413-L424
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor-related Weak Inducer of Apoptosis Augments Matrix Metalloproteinase 9 (MMP-9) Production in Skeletal Muscle through the Activation of Nuclear Factor-{kappa}B-inducing Kinase and p38 Mitogen-activated Protein Kinase: A POTENTIAL ROLE OF MMP-9 IN MYOPATHY.
H. Li, A. Mittal, P. K. Paul, M. Kumar, D. S. Srivastava, S. C. Tyagi, and A. Kumar (2009)
J. Biol. Chem. 284, 4439-4450
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor-{alpha} Augments Matrix Metalloproteinase-9 Production in Skeletal Muscle Cells through the Activation of Transforming Growth Factor- -activated Kinase 1 (TAK1)-dependent Signaling Pathway.
A. K. Srivastava, X. Qin, N. Wedhas, M. Arnush, T. A. Linkhart, R. B. Chadwick, and A. Kumar (2007)
J. Biol. Chem. 282, 35113-35124
   Abstract »    Full Text »    PDF »
Vascular: Uniaxial stretch-induced regulation of mitogen-activated protein kinase, Akt and p70 S6 kinase in the ageing Fischer 344 x Brown Norway rat aorta.
K. M. Rice, D. H. Desai, D. L. Preston, P. S. Wehner, and E. R. Blough (2007)
Exp Physiol 92, 963-970
   Abstract »    Full Text »    PDF »
Fibroblast Growth Factor Inducible 14 (Fn14) Is Required for the Expression of Myogenic Regulatory Factors and Differentiation of Myoblasts into Myotubes: EVIDENCE FOR TWEAK-INDEPENDENT FUNCTIONS OF Fn14 DURING MYOGENESIS.
C. Dogra, S. L. Hall, N. Wedhas, T. A. Linkhart, and A. Kumar (2007)
J. Biol. Chem. 282, 15000-15010
   Abstract »    Full Text »    PDF »
Regulation of the nuclear export of the transcription factor NFATc1 by protein kinases after slow fibre type electrical stimulation of adult mouse skeletal muscle fibres.
T. Shen, Z. Cseresnyes, Y. Liu, W. R. Randall, and M. F. Schneider (2007)
J. Physiol. 579, 535-551
   Abstract »    Full Text »    PDF »
Tumor Necrosis Factor-like Weak Inducer of Apoptosis Inhibits Skeletal Myogenesis through Sustained Activation of Nuclear Factor-{kappa}B and Degradation of MyoD Protein.
C. Dogra, H. Changotra, S. Mohan, and A. Kumar (2006)
J. Biol. Chem. 281, 10327-10336
   Abstract »    Full Text »    PDF »
Impact of sarcoglycan complex on mechanical signal transduction in murine skeletal muscle.
E. R. Barton (2006)
Am J Physiol Cell Physiol 290, C411-C419
   Abstract »    Full Text »    PDF »
Inhibition of mechanosensitive cation channels inhibits myogenic differentiation by suppressing the expression of myogenic regulatory factors and caspase-3 activity.
N. Wedhas, H. J. Klamut, C. Dogra, A. K. Srivastava, S. Mohan, and A. Kumar (2005)
FASEB J 19, 1986-1997
   Abstract »    Full Text »    PDF »
Activation of p38 MAPK{alpha} by extracellular pressure mediates the stimulation of macrophage phagocytosis by pressure.
H. Shiratsuchi and M. D. Basson (2005)
Am J Physiol Cell Physiol 288, C1083-C1093
   Abstract »    Full Text »    PDF »
{gamma}-Sarcoglycan deficiency increases cell contractility, apoptosis and MAPK pathway activation but does not affect adhesion.
M. A. Griffin, H. Feng, M. Tewari, P. Acosta, M. Kawana, H. L. Sweeney, and D. E. Discher (2005)
J. Cell Sci. 118, 1405-1416
   Abstract »    Full Text »    PDF »
Inhibition of cross-bridge formation has no effect on contraction-associated phosphorylation of p38 MAPK in mouse skeletal muscle.
J. N. Dentel, S. G. Blanchard, D. P. Ankrapp, L. R. McCabe, and R. W. Wiseman (2005)
Am J Physiol Cell Physiol 288, C824-C830
   Abstract »    Full Text »    PDF »
Intracellular signaling specificity in response to uniaxial vs. multiaxial stretch: implications for mechanotransduction.
T. A. Hornberger, D. D. Armstrong, T. J. Koh, T. J. Burkholder, and K. A. Esser (2005)
Am J Physiol Cell Physiol 288, C185-C194
   Abstract »    Full Text »    PDF »
Cyclic mechanical strain inhibits skeletal myogenesis through activation of focal adhesion kinase, Rac-1 GTPase, and NF-{kappa}B transcription factor.
A. KUMAR, R. MURPHY, P. ROBINSON, L. WEI, and A. M. BORIEK (2004)
FASEB J 18, 1524-1535
   Abstract »    Full Text »    PDF »
Shikonins, Phytocompounds from Lithospermum erythrorhizon, Inhibit the Transcriptional Activation of Human Tumor Necrosis Factor {alpha} Promoter in Vivo.
V. Staniforth, S.-Y. Wang, L.-F. Shyur, and N.-S. Yang (2004)
J. Biol. Chem. 279, 5877-5885
   Abstract »    Full Text »    PDF »
Loss of dystrophin causes aberrant mechanotransduction in skeletal muscle fibers.
A. KUMAR, N. KHANDELWAL, R. MALYA, M. B. REID, and A. M. BORIEK (2004)
FASEB J 18, 102-113
   Abstract »    Full Text »    PDF »
Inhibition of MAP/ERK kinase prevents IGF-I-induced hypertrophy in rat muscles.
F. Haddad and G. R. Adams (2004)
J Appl Physiol 96, 203-210
   Abstract »    Full Text »    PDF »
Mechanical stretch activates nuclear factor-kappaB, activator protein-1, and mitogen-activated protein kinases in lung parenchyma: implications in asthma.
A. KUMAR, S. LNU, R. MALYA, D. BARRON, J. MOORE, D. B. CORRY, and A. M. BORIEK (2003)
FASEB J 17, 1800-1811
   Abstract »    Full Text »    PDF »
Genetic Models in Applied Physiology: Selected Contribution: Merosin deficiency leads to alterations in passive and active skeletal muscle mechanics.
S. R. Jannapureddy, N. D. Patel, W. Hwang, and A. M. Boriek (2003)
J Appl Physiol 94, 2524-2533
   Abstract »    Full Text »    PDF »
CCAAT/Enhancer-binding Protein and Activator Protein-1 Transcription Factors Regulate the Expression of Interleukin-8 through the Mitogen-activated Protein Kinase Pathways in Response to Mechanical Stretch of Human Airway Smooth Muscle Cells.
A. Kumar, A. J. Knox, and A. M. Boriek (2003)
J. Biol. Chem. 278, 18868-18876
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882