Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

J. Biol. Chem. 277 (52): 50226-50229

© 2002 by The American Society for Biochemistry and Molecular Biology, Inc.

ACCELERATED PUBLICATION
Dampening of Cytosolic Ca2+ Oscillations on Propagation to Nucleus*

Pablo ChameroDagger , Carlos Villalobos§, María Teresa Alonso, and Javier García-Sancho

From the Instituto de Biología y Genética Molecular (IBGM), Universidad de Valladolid and Consejo Superior de Investigaciones Científicas (CSIC), Departamento de Fisiología y Bioquímica, Facultad de Medicina, E-47005 Valladolid, Spain

Ca2+ signals may regulate gene expression. The increase of the cytosolic Ca2+ concentration ([Ca2+]c) promotes activation and/or nuclear import of some transcription factors, but others require the increase of the nuclear Ca2+ concentration ([Ca2+]N) for activation. Whether the nuclear envelope may act as a diffusion barrier for propagation of [Ca2+]c signals remains controversial. We have studied the spreading of Ca2+ from the cytosol to the nucleus by comparing the cytosolic and the nuclear Ca2+ signals reported by targeted aequorins in adrenal chromaffin, PC12, and GH3 pituitary cells. Strong stimulation of either Ca2+ entry (by depolarization with high K+ or acethylcholine) or Ca2+ release from the intracellular Ca2+ stores (by stimulation with caffeine, UTP, bradykinin, or thyrotropin-releasing hormone (TRH)) produced similar Ca2+ signals in cytosol and nucleus. In contrast, both spontaneous and TRH-stimulated oscillations of cytosolic Ca2+ in single GH3 cells were considerably dampened during propagation to the nucleus. These results are consistent with the existence of a kinetic barrier that filters high frequency physiological [Ca2+]c oscillations without disturbing sustained [Ca2+]c increases. Thus, encoding of the Ca2+ signal may allow differential control of Ca2+-dependent mechanisms located at either the cytosol or the nucleus.


* This work was supported by a grant from the Spanish Ministerio de Ciencia y Tecnología (MCyT; BFI2001-2073).The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Dagger Holds a predoctoral fellowship from the Basque Government.

§ Fellow of the Ramón y Cajal Program of MCyT.

To whom correspondence should be addressed: IBGM, Dept. Fisiología, Facultad de Medicina, E-47005 Valladolid, Spain. Tel.: 34-983-423085; Fax: 34-983-423588; E-mail: jgsancho@ibgm.uva.es.


Copyright © 2002 by The American Society for Biochemistry and Molecular Biology, Inc.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Calpain-1 Cleaves Rad21 To Promote Sister Chromatid Separation.
A. K. Panigrahi, N. Zhang, Q. Mao, and D. Pati (2011)
Mol. Cell. Biol. 31, 4335-4347
   Abstract »    Full Text »    PDF »
An update on nuclear calcium signalling.
M. D. Bootman, C. Fearnley, I. Smyrnias, F. MacDonald, and H. L. Roderick (2009)
J. Cell Sci. 122, 2337-2350
   Abstract »    Full Text »    PDF »
Regulation of Nuclear Ca2+ Signaling by Translocation of the Ca2+ Messenger Synthesizing Enzyme ADP-ribosyl Cyclase during Neuronal Depolarization.
S. Bezin, G. Charpentier, H. C. Lee, G. Baux, P. Fossier, and J.-M. Cancela (2008)
J. Biol. Chem. 283, 27859-27870
   Abstract »    Full Text »    PDF »
Calcium signaling and exocytosis in adrenal chromaffin cells..
A. G. Garcia, A. M. Garcia-De-Diego, L. Gandia, R. Borges, and J. Garcia-Sancho (2006)
Physiol Rev 86, 1093-1131
   Abstract »    Full Text »    PDF »
Modulation of [Ca2+]i Signaling Dynamics and Metabolism by Perinuclear Mitochondria in Mouse Parotid Acinar Cells.
J. I. E. Bruce, D. R. Giovannucci, G. Blinder, T. J. Shuttleworth, and D. I. Yule (2004)
J. Biol. Chem. 279, 12909-12917
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882