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J. Biol. Chem. 280 (2): 1438-1447

© 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

Phospholipase Cβ2 Binds to and Inhibits Phospholipase C{delta}1*Formula

Yuanjian Guo, Mario Rebecchi, , and Suzanne Scarlata{ddagger}

Department of Physiology and Biophysics and the Department of Anesthesiology, State University of New York, Stony Brook, New York 11794-8661

Abstract: Phospholipase Cβ (PLCβ) isoforms, which are under the control of Gαq and Gβ{gamma} subunits, generate Ca2+ signals induced by a broad array of extracellular agonists, whereas PLC{delta} isoforms depend on a rise in cytosolic Ca2+ for their activation. Here we find that PLCβ2 binds strongly to PLC{delta}1 and inhibits its catalytic activity in vitro and in living cells. In vitro, this PLC complex can be disrupted by increasing concentrations of free Gβ{gamma} subunits. Such competition has consequences for signaling, because in HEK293 cells PLCβ2 suppresses elevated basal [Ca2+] and inositol phosphates levels and the sustained agonist-induced elevation of Ca2+ levels caused by PLC{delta}1. Also, expression of both PLCs results in a synergistic release of [Ca2+] upon stimulation in A10 cells. These results support a model in which PLCβ2 suppresses the basal catalytic activity of PLC{delta}1, which is relieved by binding of Gβ{gamma} subunits to PLCβ2 allowing for amplified calcium signals.

Received for publication July 7, 2004. Revision received October 25, 2004.

{ddagger} To whom correspondence to should be addressed. Tel.: 631-444-3071; Fax: 631-444-3432; E-mail: Suzanne.Scarlata{at}

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