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J. Cell Biol. 162 (5): 781-788

Copyright © 2003 by the Rockefeller University Press.

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Local signaling by the EGF receptor

Stephan J. Kempiak1, Shu-Chin Yip2, Jonathan M. Backer2, and Jeffrey E. Segall1

1 Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, New York, NY 10461
2 Department of Molecular Pharmacology, Albert Einstein College of Medicine, New York, NY 10461

Address correspondence to J.E. Segall, Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, 1300 Morris Park Ave., New York, NY 10461. Tel.: (718) 430-4237. Fax: (718) 430-8996. email: segall{at}aecom.yu.edu

Abstract: Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of PI3-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.

Key Words: chemotaxis; receptor; epidermal growth factor; signal transduction; EGF bead

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