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J. Physiol. 587 (1): 211-217

Rapid Report

Age-related differences in the dose–response relationship of muscle protein synthesis to resistance exercise in young and old men

Vinod Kumar1, Anna Selby1, Debbie Rankin1, Rekha Patel1, Philip Atherton1, Wulf Hildebrandt1, John Williams2, Kenneth Smith1, Olivier Seynnes3, Natalie Hiscock4, and Michael J. Rennie1

1 University of Nottingham, School of Graduate Entry Medicine and Health, Derby DE22 3DT, UK
2 Anaesthetic Department, Derby Hospitals NHS Foundation Trust, Derby DE22 3DT, UK
3 IRM, Manchester Metropolitan University, Manchester M1 5GD, UK
4 Unilever Discover R & D, Colworth Science Park, Sharnbrook MK44 1LQ, UK

Abstract: We investigated how myofibrillar protein synthesis (MPS) and muscle anabolic signalling were affected by resistance exercise at 20–90% of 1 repetition maximum (1 RM) in two groups (25 each) of post-absorptive, healthy, young (24 ± 6 years) and old (70 ± 5 years) men with identical body mass indices (24 ± 2 kg m–2). We hypothesized that, in response to exercise, anabolic signalling molecule phosphorylation and MPS would be modified in a dose-dependant fashion, but to a lesser extent in older men. Vastus lateralis muscle was sampled before, immediately after, and 1, 2 and 4 h post-exercise. MPS was measured by incorporation of [1,2-13C] leucine (gas chromatography–combustion–mass spectrometry using plasma [1,2-13C]{alpha}-ketoisocaparoate as surrogate precursor); the phosphorylation of p70 ribosomal S6 kinase (p70s6K) and eukaryotic initiation factor 4E binding protein 1 (4EBP1) was measured using Western analysis with anti-phosphoantibodies. In each group, there was a sigmoidal dose–response relationship between MPS at 1–2 h post-exercise and exercise intensity, which was blunted (P < 0.05) in the older men. At all intensities, MPS fell in both groups to near-basal values by 2–4 h post-exercise. The phosphorylation of p70s6K and 4EBP1 at 60–90% 1 RM was blunted in older men. At 1 h post-exercise at 60–90% 1 RM, p70s6K phosphorylation predicted the rate of MPS at 1–2 h post-exercise in the young but not in the old. The results suggest that in the post-absorptive state: (i) MPS is dose dependant on intensity rising to a plateau at 60–90% 1 RM; (ii) older men show anabolic resistance of signalling and MPS to resistance exercise.

Received for publication 7 October 2008. Revision received 10 November 2008. Accepted for publication 10 November 2008.

Corresponding author M. J. Rennie: University of Nottingham, Division of Clinical Physiology, School of Graduate Entry Medicine and Health, City Hospital, Uttoxeter Road, Derby, DE22 3DT, UK.  Email: michael.rennie{at}nottingham.ac.uk

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