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Mol. Biol. Cell 15 (5): 2492-2508

Copyright © 2004 by The American Society for Cell Biology.

The Chemokine Receptor D6 Constitutively Traffics to and from the Cell Surface to Internalize and Degrade Chemokines

Michele Weber *, Emma Blair *, Clare V. Simpson * {dagger}, Maureen O'Hara * {dagger}, Paul E. Blackburn * {dagger}, Antal Rot {ddagger}, Gerard J. Graham * §, and Robert J.B. Nibbs * § ||

* The Cancer Research UK Beatson Laboratories, The Beatson Institute for Cancer Research, Glasgow, United Kingdom G61 1BD
§ The Division of Immunology, Infection, and Inflammation, Glasgow University, Glasgow, United Kingdom G11 6NT
{dagger} Department of Chemistry, Glasgow University, Glasgow, United Kingdom G11 6NT {ddagger} Novartis Forschunginstitut, Vienna, A-1235 Austria

Received for publication September 2, 2003. Revision received February 10, 2004. Accepted for publication February 12, 2004.

Monitoring Editor: Howard Riezman

Abstract: The D6 heptahelical membrane protein, expressed by lymphatic endothelial cells, is able to bind with high affinity to multiple proinflammatory CC chemokines. However, this binding does not allow D6 to couple to the signaling pathways activated by typical chemokine receptors such as CC-chemokine receptor-5 (CCR5). Here, we show that D6, like CCR5, can rapidly internalize chemokines. However, D6-internalized chemokines are more effectively retained intracellularly because they more readily dissociate from the receptor during vesicle acidification. These chemokines are then degraded while the receptor recycles to the cell surface. Interestingly, D6-mediated chemokine internalization occurs without bringing about a reduction in cell surface D6 levels. This is possible because unlike CCR5, D6 is predominantly localized in recycling endosomes capable of trafficking to and from the cell surface in the absence of ligand. When chemokine is present, it can enter the cells associated with D6 already destined for internalization. By this mechanism, D6 can target chemokines for degradation without the necessity for cell signaling, and without desensitizing the cell to subsequent chemokine exposure.

Article published online ahead of print. Mol. Biol. Cell 10.1091/mbc.E03–09–0634. Article and publication date are available at www.molbiolcell.org/cgi/doi/10.1091/mbc.E03–09–0634.

Abbreviations used: 7-TM, seven-transmembrane; CCR, CC-chemokine receptor; CCL, CC-chemokine ligand; CMV, cytomegalovirus; DARC, Duffy antigen receptor for chemokines; GFP, green fluorescent protein; h, human; HEK, human embryonic kidney; HA, hemagglutinin; LEC, lymphatic endothelial cell; m, murine; PBS, phosphate buffered saline; PE, phycoerythrin; PFA, paraformaldehyde; S-PE, Streptavidin-PE; TCA, trichloroacetic acid; TRITC, tetramethylrhodamine isothiocyanate.


Online version of this article contains supporting material. Online version is available at www.molbiolcell.org.

|| Corresponding author. E-mail address: r.nibbs{at}beatson.gla.ac.uk.

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