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PLANT CELL 18 (8): 1975-1990

Copyright © 2006 by the American Society of Plant Physiologists.

CONSTITUTIVELY PHOTOMORPHOGENIC1 Is Required for the UV-B Response in Arabidopsis[W]

Attila Oravecza,b,1, Alexander Baumanna,1, Zoltán Mátéb, Agnieszka Brzezinskaa, Jean Molinierc, Edward J. Oakeleyc, Éva Ádámd, Eberhard Schäfera, Ferenc Nagyb,d, and Roman Ulma,2

a Institute of Biology II/Botany, University of Freiburg, D-79104 Freiburg, Germany
b Institute of Plant Biology, Agricultural Biotechnological Center, Szent-Györgyi A 4, H-2101 Gödöllo, Hungary
c Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland
d Institute of Plant Biology, Biological Research Center, H-6726 Szeged, Hungary

2 To whom correspondence should be addressed. E-mail roman.ulm{at}; fax 49-761-203-2612.

Abstract: CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1) is a negative regulator of photomorphogenesis in Arabidopsis thaliana. COP1 functions as an E3 ubiquitin ligase, targeting select proteins for proteasomal degradation in plants as well as in mammals. Among its substrates is the basic domain/leucine zipper (bZIP) transcription factor ELONGATED HYPOCOTYL5 (HY5), one of the key regulators of photomorphogenesis under all light qualities, including UV-B responses required for tolerance to this environmental threat. Here, we report that, in contrast with the situation in visible light, COP1 is a critical positive regulator of responses to low levels of UV-B. We show that in the cop1-4 mutant, flavonoid accumulation and genome-wide expression changes in response to UV-B are blocked to a large extent. COP1 is required for HY5 gene activation, and both COP1 and HY5 proteins accumulate in the nucleus under supplementary UV-B. SUPPRESSOR OF PHYTOCHROME A-105 family proteins (SPA1 to SPA4) that are required for COP1 function in dark and visible light are not essential in the response to UV-B. We conclude that COP1 performs a specific and novel role in the plants' photomorphogenic response to UV-B, coordinating HY5-dependent and -independent pathways, which eventually results in UV-B tolerance.

Ultraviolet-B-Induced Stomatal Closure in Arabidopsis Is Regulated by the UV RESISTANCE LOCUS8 Photoreceptor in a Nitric Oxide-Dependent Mechanism.
V. Tossi, L. Lamattina, G. I. Jenkins, and R. O. Cassia (2014)
Plant Physiology 164, 2220-2230
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Rapid Decline in Nuclear COSTITUTIVE PHOTOMORPHOGENESIS1 Abundance Anticipates the Stabilization of Its Target ELONGATED HYPOCOTYL5 in the Light.
M. Pacin, M. Legris, and J. J. Casal (2014)
Plant Physiology 164, 1134-1138
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Role of Arabidopsis UV RESISTANCE LOCUS 8 in Plant Growth Reduction under Osmotic Stress and Low Levels of UV-B.
R. Fasano, N. Gonzalez, A. Tosco, F. Dal Piaz, T. Docimo, R. Serrano, S. Grillo, A. Leone, and D. Inze (2014)
Mol Plant
   Abstract »    Full Text »    PDF »
The UV-B Photoreceptor UVR8: From Structure to Physiology.
G. I. Jenkins (2014)
PLANT CELL 26, 21-37
   Abstract »    Full Text »    PDF »
Constitutively active UVR8 photoreceptor variant in Arabidopsis.
M. Heijde, M. Binkert, R. Yin, F. Ares-Orpel, L. Rizzini, E. Van De Slijke, G. Persiau, J. Nolf, K. Gevaert, G. De Jaeger, et al. (2013)
PNAS 110, 20326-20331
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Z-Box Binding Transcription Factors (ZBFs): A New Class of Transcription Factors in Arabidopsis Seedling Development.
S. N. Gangappa, A. K. Srivastava, J. P. Maurya, H. Ram, and S. Chattopadhyay (2013)
Mol Plant 6, 1758-1768
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M. Jamshidiha, H. Ishida, C. Sutherland, J. L. Gifford, M. P. Walsh, and H. J. Vogel (2013)
J. Biol. Chem. 288, 32036-32049
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Conversion from CUL4-based COP1-SPA E3 apparatus to UVR8-COP1-SPA complexes underlies a distinct biochemical function of COP1 under UV-B.
X. Huang, X. Ouyang, P. Yang, O. S. Lau, L. Chen, N. Wei, and X. W. Deng (2013)
PNAS 110, 16669-16674
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Repression of Growth Regulating Factors by the MicroRNA396 Inhibits Cell Proliferation by UV-B Radiation in Arabidopsis Leaves.
R. Casadevall, R. E. Rodriguez, J. M. Debernardi, J. F. Palatnik, and P. Casati (2013)
PLANT CELL 25, 3570-3583
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Heat Shock-Induced Fluctuations in Clock and Light Signaling Enhance Phytochrome B-Mediated Arabidopsis Deetiolation.
E. Karayekov, R. Sellaro, M. Legris, M. J. Yanovsky, and J. J. Casal (2013)
PLANT CELL 25, 2892-2906
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Photomorphogenesis, B-Box Transcription Factors, and the Legacy of Magnus Holm.
C. Bowler, J. Botto, and X.-W. Deng (2013)
PLANT CELL 25, 1192-1195
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Multiple Roles for UV RESISTANCE LOCUS8 in Regulating Gene Expression and Metabolite Accumulation in Arabidopsis under Solar Ultraviolet Radiation.
L. O. Morales, M. Brosche, J. Vainonen, G. I. Jenkins, J. J. Wargent, N. Sipari, A. Strid, A. V. Lindfors, R. Tegelberg, and P. J. Aphalo (2013)
Plant Physiology 161, 744-759
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Reversion of the Arabidopsis UV-B photoreceptor UVR8 to the homodimeric ground state.
M. Heijde and R. Ulm (2013)
PNAS 110, 1113-1118
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Rapid Reversion from Monomer to Dimer Regenerates the Ultraviolet-B Photoreceptor UV RESISTANCE LOCUS8 in Intact Arabidopsis Plants.
M. Heilmann and G. I. Jenkins (2013)
Plant Physiology 161, 547-555
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Arabidopsis FHY3 and HY5 Positively Mediate Induction of COP1 Transcription in Response to Photomorphogenic UV-B Light.
X. Huang, X. Ouyang, P. Yang, O. S. Lau, G. Li, J. Li, H. Chen, and X. W. Deng (2012)
PLANT CELL 24, 4590-4606
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C-terminal region of the UV-B photoreceptor UVR8 initiates signaling through interaction with the COP1 protein.
C. Cloix, E. Kaiserli, M. Heilmann, K. J. Baxter, B. A. Brown, A. O'Hara, B. O. Smith, J. M. Christie, and G. I. Jenkins (2012)
PNAS 109, 16366-16370
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In Vivo Function of Tryptophans in the Arabidopsis UV-B Photoreceptor UVR8.
A. O'Hara and G. I. Jenkins (2012)
PLANT CELL 24, 3755-3766
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The COP9 Signalosome: Its Regulation of Cullin-Based E3 Ubiquitin Ligases and Role in Photomorphogenesis.
C. D. Nezames and X. W. Deng (2012)
Plant Physiology 160, 38-46
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Canopy Light and Plant Health.
C. L. Ballare, C. A. Mazza, A. T. Austin, and R. Pierik (2012)
Plant Physiology 160, 145-155
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Mutation of Arabidopsis HY1 causes UV-C hypersensitivity by impairing carotenoid and flavonoid biosynthesis and the down-regulation of antioxidant defence.
Y. Xie, D. Xu, W. Cui, and W. Shen (2012)
J. Exp. Bot. 63, 3869-3883
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UVR8 Mediates UV-B-Induced Arabidopsis Defense Responses against Botrytis cinerea by Controlling Sinapate Accumulation.
P. V. Demkura and C. L. Ballare (2012)
Mol Plant 5, 642-652
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Plant UVR8 Photoreceptor Senses UV-B by Tryptophan-Mediated Disruption of Cross-Dimer Salt Bridges.
J. M. Christie, A. S. Arvai, K. J. Baxter, M. Heilmann, A. J. Pratt, A. O'Hara, S. M. Kelly, M. Hothorn, B. O. Smith, K. Hitomi, et al. (2012)
Science 335, 1492-1496
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Regulation of Root Greening by Light and Auxin/Cytokinin Signaling in Arabidopsis.
K. Kobayashi, S. Baba, T. Obayashi, M. Sato, K. Toyooka, M. Keranen, E.-M. Aro, H. Fukaki, H. Ohta, K. Sugimoto, et al. (2012)
PLANT CELL 24, 1081-1095
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Integration of low temperature and light signaling during cold acclimation response in Arabidopsis.
R. Catala, J. Medina, and J. Salinas (2011)
PNAS 108, 16475-16480
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The GreenCut2 Resource, a Phylogenomically Derived Inventory of Proteins Specific to the Plant Lineage.
S. J. Karpowicz, S. E. Prochnik, A. R. Grossman, and S. S. Merchant (2011)
J. Biol. Chem. 286, 21427-21439
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Perception of UV-B by the Arabidopsis UVR8 Protein.
L. Rizzini, J.-J. Favory, C. Cloix, D. Faggionato, A. O'Hara, E. Kaiserli, R. Baumeister, E. Schafer, F. Nagy, G. I. Jenkins, et al. (2011)
Science 332, 103-106
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Genome-Wide Analysis of Light-Dependent Transcript Accumulation Patterns during Early Stages of Arabidopsis Seedling Deetiolation.
F. Peschke and T. Kretsch (2011)
Plant Physiology 155, 1353-1366
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EFO1 and EFO2, encoding putative WD-domain proteins, have overlapping and distinct roles in the regulation of vegetative development and flowering of Arabidopsis.
W. Wang, D. Yang, and K. A. Feldmann (2011)
J. Exp. Bot. 62, 1077-1088
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Negative feedback regulation of UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis.
H. Gruber, M. Heijde, W. Heller, A. Albert, H. K. Seidlitz, and R. Ulm (2010)
PNAS 107, 20132-20137
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Plant L10 Ribosomal Proteins Have Different Roles during Development and Translation under Ultraviolet-B Stress.
M. L. F. Ferreyra, A. Pezza, J. Biarc, A. L. Burlingame, and P. Casati (2010)
Plant Physiology 153, 1878-1894
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Arabidopsis Cockayne Syndrome A-Like Proteins 1A and 1B Form a Complex with CULLIN4 and Damage DNA Binding Protein 1A and Regulate the Response to UV Irradiation.
C. Zhang, H. Guo, J. Zhang, G. Guo, K. S. Schumaker, and Y. Guo (2010)
PLANT CELL 22, 2353-2369
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The Molecular and Physiological Responses of Physcomitrella patens to Ultraviolet-B Radiation.
L. Wolf, L. Rizzini, R. Stracke, R. Ulm, and S. A. Rensing (2010)
Plant Physiology 153, 1123-1134
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Karrikins enhance light responses during germination and seedling development in Arabidopsis thaliana.
D. C. Nelson, G. R. Flematti, J.-A. Riseborough, E. L. Ghisalberti, K. W. Dixon, and S. M. Smith (2010)
PNAS 107, 7095-7100
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ROOT UV-B SENSITIVE2 Acts with ROOT UV-B SENSITIVE1 in a Root Ultraviolet B-Sensing Pathway.
C. D. Leasure, H. Tong, G. Yuen, X. Hou, X. Sun, and Z.-H. He (2009)
Plant Physiology 150, 1902-1915
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Interaction of COP1 and UVR8 regulates UV-B-induced photomorphogenesis and stress acclimation in Arabidopsis.
J.-J. Favory, A. Stec, H. Gruber, L. Rizzini, A. Oravecz, M. Funk, A. Albert, C. Cloix, G. I. Jenkins, E. J. Oakeley, et al. (2009)
EMBO J. 28, 591-601
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Role of root UV-B sensing in Arabidopsis early seedling development.
H. Tong, C. D. Leasure, X. Hou, G. Yuen, W. Briggs, and Z.-H. He (2008)
PNAS 105, 21039-21044
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Hormone- and light-regulated nucleocytoplasmic transport in plants: current status.
Y. Lee, H.-S. Lee, J.-S. Lee, S.-K. Kim, and S.-H. Kim (2008)
J. Exp. Bot. 59, 3229-3245
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Biochemical Characterization of Arabidopsis Complexes Containing CONSTITUTIVELY PHOTOMORPHOGENIC1 and SUPPRESSOR OF PHYA Proteins in Light Control of Plant Development.
D. Zhu, A. Maier, J.-H. Lee, S. Laubinger, Y. Saijo, H. Wang, L.-J. Qu, U. Hoecker, and X. W. Deng (2008)
PLANT CELL 20, 2307-2323
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UV-B Signaling Pathways with Different Fluence-Rate Response Profiles Are Distinguished in Mature Arabidopsis Leaf Tissue by Requirement for UVR8, HY5, and HYH.
B. A. Brown and G. I. Jenkins (2008)
Plant Physiology 146, 576-588
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Interaction of the Arabidopsis UV-B-Specific Signaling Component UVR8 with Chromatin.
C. Cloix and G. I. Jenkins (2008)
Mol Plant 1, 118-128
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UV-B Promotes Rapid Nuclear Translocation of the Arabidopsis UV-B Specific Signaling Component UVR8 and Activates Its Function in the Nucleus.
E. Kaiserli and G. I. Jenkins (2007)
PLANT CELL 19, 2662-2673
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Genome-Wide Gene Expression Analysis Reveals a Critical Role for CRYPTOCHROME1 in the Response of Arabidopsis to High Irradiance.
T. Kleine, P. Kindgren, C. Benedict, L. Hendrickson, and A. Strand (2007)
Plant Physiology 144, 1391-1406
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PlanTAPDB, a Phylogeny-Based Resource of Plant Transcription-Associated Proteins.
S. Richardt, D. Lang, R. Reski, W. Frank, and S. A. Rensing (2007)
Plant Physiology 143, 1452-1466
   Abstract »    Full Text »    PDF »

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