Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Plant Physiology 136 (3): 3628-3638

Copyright © 2004 by the American Society of Plant Physiologists.


CELL BIOLOGY AND SIGNAL TRANSDUCTION

Downstream Divergence of the Ethylene Signaling Pathway for Harpin-Stimulated Arabidopsis Growth and Insect Defense1

Hong-Ping Dong2,3, Jianling Peng2,4, Zhilong Bao, Xiangdong Meng, Jean M. Bonasera, Guangyong Chen, Steven V. Beer, and Hansong Dong*

Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, China (H.-P.D., J.P., Z.B., G.C., H.D.); and Department of Plant Pathology, Cornell University, Ithaca, New York 14853 (X.M., J.M.B., S.V.B.)

Abstract: Ethylene (ET) signal transduction may regulate plant growth and defense, depending on which components are recruited into the pathway in response to different stimuli. We report here that the ET pathway controls both insect resistance (IR) and plant growth enhancement (PGE) in Arabidopsis (Arabidopsis thaliana) plants responding to harpin, a protein produced by a plant pathogenic bacterium. PGE may result from spraying plant tops with harpin or by soaking seeds in harpin solution; the latter especially enhances root growth. Plants treated similarly develop resistance to the green peach aphid (Myzus persicae). The salicylic acid pathway, although activated by harpin, does not lead to PGE and IR. By contrast, PGE and IR are induced in both wild-type plants and genotypes that have defects in salicylic acid signaling. In response to harpin, levels of jasmonic acid (JA) decrease, and the COI1 gene, which is indispensable for JA signal transduction, is not expressed in wild-type plants. However, PGE and IR are stimulated in the JA-resistant mutant jar1-1. In the wild type, PGE and IR develop coincidently with increases in ET levels and the expression of several genes essential for ET signaling. The ET receptor gene ETR1 is required because both phenotypes are arrested in the etr1-1 mutant. Consistently, inhibition of ET perception nullifies the induction of both PGE and IR. The signal transducer EIN2 is required for IR, and EIN5 is required for PGE because IR and PGE are impaired correspondingly in the ein2-1 and ein5-1 mutants. Therefore, harpin activates ET signaling while conscribing EIN2 and EIN5 to confer IR and PGE, respectively.


1 This work was supported by the U.S. Department of Agriculture (grants to S.V.B.) and a royalty income fund (to S.V.B.), the China National Natural Science Foundation (grant no. 30370969), the Ministry of Education of China (Century-Across Talent award no. 2002–48), and the China National 863 Plan (award no. 2002AA245011 to H.D.).

2 These authors contributed equally to the paper.

3 Present address: College of Life Sciences, Nanjing Normal University, 122 Ninghai Road, Nanjing 210097, China.

4 Present address: Institute of Genetics and Developmental Biology, Chinese Academy of Science, 1 Anwai-Datun Road, Beijing 100101, China.

Article, publication date, and citation information can be found at www.plantphysiol.org/cgi/doi/10.1104/pp.104.048900.

* Corresponding author; e-mail hsdong{at}njau.edu.cn; fax 86–(25)–84395246.

Received for publication June 30, 2004. Revision received September 11, 2004. Accepted for publication September 20, 2004.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Transgenic expression of a functional fragment of harpin protein Hpa1 in wheat induces the phloem-based defence against English grain aphid.
M. Fu, M. Xu, T. Zhou, D. Wang, S. Tian, L. Han, H. Dong, and C. Zhang (2014)
J. Exp. Bot. 65, 1439-1453
   Abstract »    Full Text »    PDF »
Tobacco TTG2 suppresses resistance to pathogens by sequestering NPR1 from the nucleus.
B. Li, R. Gao, R. Cui, B. Lu, X. Li, Y. Zhao, Z. You, S. Tian, and H. Dong (2012)
J. Cell Sci. 125, 4913-4922
   Abstract »    Full Text »    PDF »
Overexpression of a Harpin-encoding gene hrf1 in rice enhances drought tolerance.
L. Zhang, S. Xiao, W. Li, W. Feng, J. Li, Z. Wu, X. Gao, F. Liu, and M. Shao (2011)
J. Exp. Bot. 62, 4229-4238
   Abstract »    Full Text »    PDF »
Transcriptome analysis of Hpa1Xoo transformed cotton revealed constitutive expression of genes in multiple signalling pathways related to disease resistance.
W. Miao, X. Wang, C. Song, Y. Wang, Y. Ren, and J. Wang (2010)
J. Exp. Bot. 61, 4263-4275
   Abstract »    Full Text »    PDF »
Nicotiana tabacum TTG1 contributes to ParA1-induced signalling and cell death in leaf trichomes.
Y. Wang, R. Liu, L. Chen, Y. Wang, Y. Liang, X. Wu, B. Li, J. Wu, Y. Liang, X. Wang, et al. (2009)
J. Cell Sci. 122, 2673-2685
   Abstract »    Full Text »    PDF »
Tomato Protein Kinase 1b Mediates Signaling of Plant Responses to Necrotrophic Fungi and Insect Herbivory.
S. AbuQamar, M.-F. Chai, H. Luo, F. Song, and T. Mengiste (2008)
PLANT CELL 20, 1964-1983
   Abstract »    Full Text »    PDF »
AtHIPM, an Ortholog of the Apple HrpN-Interacting Protein, Is a Negative Regulator of Plant Growth and Mediates the Growth-Enhancing Effect of HrpN in Arabidopsis.
C.-S. Oh and S. V. Beer (2007)
Plant Physiology 145, 426-436
   Abstract »    Full Text »    PDF »
The Exoribonuclease XRN4 Is a Component of the Ethylene Response Pathway in Arabidopsis.
T. Potuschak, A. Vansiri, B. M. Binder, E. Lechner, R. D. Vierstra, and P. Genschik (2006)
PLANT CELL 18, 3047-3057
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882