Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Balaraj B. Menon *,
Nayan J. Sarma *,
Satish Pasula *,
Stephen J. Deminoff *,
Kristine A. Willis,
Kellie E. Barbara *,
Brenda Andrews, and
George M. Santangelo *,
*Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, MS 39406; and Department of Medical Genetics and Microbiology, University of Toronto, Toronto, ON, Canada M5S 1A8
Communicated by Michael S. Levine, University of California, Berkeley, CA, March 4, 2005
Received for publication January 10, 2005.
The recruitment model for gene activation presumes that DNAis a platform on which the requisite components of the transcriptionalmachinery are assembled. In contrast to this idea, we show herethat Rap1/Gcr1/Gcr2 transcriptional activation in yeast cellsoccurs through a large anchored protein platform, the Nup84nuclear pore subcomplex. Surprisingly, Nup84 and associatedsubcomplex components activate transcription themselves in vivowhen fused to a heterologous DNA-binding domain. The Rap1 coactivatorsGcr1 and Gcr2 form an important bridge between the yeast nuclearpore complex and the transcriptional machinery. Nucleoporinactivation may be a widespread eukaryotic phenomenon, becauseit was first detected as a consequence of oncogenic rearrangementsin acute myeloid leukemia and related syndromes in humans. Thesechromosomal translocations fuse a homeobox DNA-binding domainto the human homolog (hNup98) of a transcriptionally activecomponent of the yeast Nup84 subcomplex. We conclude that Rap1target genes are activated by moving to contact compartmentalizednuclear assemblages, rather than through recruitment of therequisite factors to chromatin by means of diffusion. We termthis previously undescribed mechanism "reverse recruitment"and discuss the possibility that it is a central feature ofeukaryotic gene regulation. Reverse recruitment stipulates thatactivators work by bringing the DNA to an nuclear pore complex-tetheredplatform of assembled transcriptional machine components.
A regression model approach to enable cell morphology correction in high-throughput flow cytometry.
T. A. Knijnenburg, O. Roda, Y. Wan, G. P. Nolan, J. D. Aitchison, and I. Shmulevich (2014)
Mol Syst Biol
|Abstract »|Full Text »|PDF »
The Hog1 Stress-activated Protein Kinase Targets Nucleoporins to Control mRNA Export upon Stress.
S. Regot, E. de Nadal, S. Rodriguez-Navarro, A. Gonzalez-Novo, J. Perez-Fernandez, O. Gadal, G. Seisenbacher, G. Ammerer, and F. Posas (2013)
J. Biol. Chem.
|Abstract »|Full Text »|PDF »
Arabidopsis thaliana VOZ (Vascular plant One-Zinc finger) transcription factors are required for proper regulation of flowering time.