Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

PNAS 103 (11): 4210-4215

Copyright © 2006 by the National Academy of Sciences.


BIOLOGICAL SCIENCES / IMMUNOLOGY

Selective potentiation of Stat-dependent gene expression by collaborator of Stat6 (CoaSt6), a transcriptional cofactor

Shreevrat Goenka*, and Mark Boothby*,{dagger},{ddagger}

*Department of Microbiology and Immunology and {dagger}Division of Rheumatology, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN 37232-2363

Edited by Philippa Marrack, National Jewish Medical and Research Center, Denver, CO, and approved January 17, 2006

Received for publication August 11, 2005.

Abstract: The molecular mechanisms by which transcription is selectively activated and precisely controlled by signal transducer and activator of transcription (Stat) factors represent a central issue in cytokine-mediated cellular responses. Stat6 mediates responses to IL-4 and antagonizes Stat1 activated by IFN-{gamma}. We have discovered that Stat6 binds to collaborator of Stat6 (CoaSt6), a protein that lacks conventional coactivator motifs but contains three iterations of a domain found in the variant histone macroH2A. Although macroH2A participates in transcriptional silencing, the macro domains of CoaSt6 increased IL-4-induced gene expression. Moreover, CoaSt6 amplified Stat6-mediated but not IFN-{gamma}-induced gene expression, providing evidence of a selective coregulator of Stat-mediated gene transcription.

Key Words: coregulator • Stat1 • cytokine • macro domain


Author contributions: S.G. and M.B. designed research; S.G. performed research; S.G. contributed new reagents/analytic tools; S.G. and M.B. analyzed data; and S.G. and M.B. wrote the paper.

Conflict of interest statement: No conflicts declared.

This paper was submitted directly (Track II) to the PNAS office.

Data deposition: The sequence reported in this paper has been deposited in the GenBank database (accession no. DQ372930).

{ddagger}To whom correspondence should be addressed. E-mail: mark.boothby{at}vanderbilt.edu

© 2006 by The National Academy of Sciences of the USA


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
B Cell-Intrinsic and -Extrinsic Regulation of Antibody Responses by PARP14, an Intracellular (ADP-Ribosyl)Transferase.
S. H. Cho, A. Raybuck, M. Wei, J. Erickson, K. T. Nam, R. G. Cox, A. Trochtenberg, J. W. Thomas, J. Williams, and M. Boothby (2013)
J. Immunol. 191, 3169-3178
   Abstract »    Full Text »    PDF »
BAL1/ARTD9 represses the anti-proliferative and pro-apoptotic IFN{gamma}-STAT1-IRF1-p53 axis in diffuse large B-cell lymphoma.
R. Camicia, S. B. Bachmann, H. C. Winkler, M. Beer, M. Tinguely, E. Haralambieva, and P. O. Hassa (2013)
J. Cell Sci. 126, 1969-1980
   Abstract »    Full Text »    PDF »
STAT6-Dependent Regulation of Th9 Development.
R. Goswami, R. Jabeen, R. Yagi, D. Pham, J. Zhu, S. Goenka, and M. H. Kaplan (2012)
J. Immunol. 188, 968-975
   Abstract »    Full Text »    PDF »
Glycolytic rate and lymphomagenesis depend on PARP14, an ADP ribosyltransferase of the B aggressive lymphoma (BAL) family.
S. H. Cho, A. K. Ahn, P. Bhargava, C.-H. Lee, C. M. Eischen, O. McGuinness, and M. Boothby (2011)
PNAS 108, 15972-15977
   Abstract »    Full Text »    PDF »
PARP-14 Functions as a Transcriptional Switch for Stat6-dependent Gene Activation.
P. Mehrotra, J. P. Riley, R. Patel, F. Li, L. Voss, and S. Goenka (2011)
J. Biol. Chem. 286, 1767-1776
   Abstract »    Full Text »    PDF »
The Paralogous Genes RADICAL-INDUCED CELL DEATH1 and SIMILAR TO RCD ONE1 Have Partially Redundant Functions during Arabidopsis Development.
S. Teotia and R. S. Lamb (2009)
Plant Physiology 151, 180-198
   Abstract »    Full Text »    PDF »
PARP-14, a member of the B aggressive lymphoma family, transduces survival signals in primary B cells.
S. H. Cho, S. Goenka, T. Henttinen, P. Gudapati, A. Reinikainen, C. M. Eischen, R. Lahesmaa, and M. Boothby (2009)
Blood 113, 2416-2425
   Abstract »    Full Text »    PDF »
The single-macro domain protein LRP16 is an essential cofactor of androgen receptor.
J Yang, Y-L Zhao, Z-Q Wu, Y-L Si, Y-G Meng, X-B Fu, Y-M Mu, and W-D Han (2009)
Endocr. Relat. Cancer 16, 139-153
   Abstract »    Full Text »    PDF »
The Poly(ADP-ribose) Polymerase PARP-1 Is Required for Oxidative Stress-induced TRPM2 Activation in Lymphocytes.
B. Buelow, Y. Song, and A. M. Scharenberg (2008)
J. Biol. Chem. 283, 24571-24583
   Abstract »    Full Text »    PDF »
Crif1 is a novel transcriptional coactivator of STAT3.
M.-c. Kwon, B.-K. Koo, J.-S. Moon, Y.-Y. Kim, K. C. Park, N.-S. Kim, M. Y. Kwon, M.-P. Kong, K.-J. Yoon, S.-K. Im, et al. (2008)
EMBO J. 27, 642-653
   Abstract »    Full Text »    PDF »
Regulation of Phosphoglucose Isomerase/Autocrine Motility Factor Activities by the Poly(ADP-Ribose) Polymerase Family-14.
T. Yanagawa, T. Funasaka, S. Tsutsumi, H. Hu, H. Watanabe, and A. Raz (2007)
Cancer Res. 67, 8682-8689
   Abstract »    Full Text »    PDF »
Collaborator of Stat6 (CoaSt6)-associated Poly(ADP-ribose) Polymerase Activity Modulates Stat6-dependent Gene Transcription.
S. Goenka, S. H. Cho, and M. Boothby (2007)
J. Biol. Chem. 282, 18732-18739
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882