Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Jon M. Jacobs,
Ronald J. Moore,
David G. Camp, II,
Richard D. Smith, and
Richard L. Klemke,
Department of Pathology and Moores Cancer Center, University of California at San Diego, La Jolla, CA 92093; and Biological Sciences Division, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99354
Edited by Peter N. Devreotes, Johns Hopkins University School of Medicine, Baltimore, MD, and approved April 10, 2007
Received for publication February 6, 2007.
Cell movement requires morphological polarization characterizedby formation of a leading pseudopodium (PD) at the front anda trailing rear at the back. However, little is known abouthow protein networks are spatially integrated to regulate thisprocess at the system level. Here, we apply global proteomeprofiling in combination with newly developed quantitative phosphoproteomicsapproaches for comparative analysis of the cell body (CB) andPD proteome of chemotactic cells. The spatial relationship of3,509 proteins and 228 distinct sites of phosphorylation weremapped revealing networks of signaling proteins that partitionto the PD and/or the CB compartments. The major network representedin the PD includes integrin signaling, actin regulatory, andaxon guidance proteins, whereas the CB consists of DNA/RNA metabolism,cell cycle regulation, and structural maintenance. Our findingsprovide insight into the spatial organization of signaling networksthat control cell movement and provide a comprehensive system-wideprofile of proteins and phosphorylation sites that control cellpolarization.
Author contributions: Y.W. and S.-J.D. contributed equally tothis work; Y.W., S.-J.D., W.W., and R.L.K. designed research;Y.W., S.-J.D., and W.W. performed research; Y.W., S.-J.D., F.Y.,D.G.C., R.D.S., and R.L.K. contributed new reagents/analytictools; Y.W., S.-J.D., J.M.J., W.-J.Q., R.J.M., and R.L.K. analyzeddata; and Y.W., S.-J.D., J.M.J., R.D.S., and R.L.K. wrote thepaper.