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PNAS 106 (1): 280-285

Copyright © 2009 by the National Academy of Sciences.


BIOLOGICAL SCIENCES / MICROBIOLOGY

Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis

Daniel Lópeza, Michael A. Fischbachb, Frances Chuc, Richard Losickc,1, and Roberto Koltera,1

aDepartment of Microbiology and Molecular Genetics and bDepartment of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115; and cDepartment of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138

Contributed by Richard Losick, November 4, 2008

Received for publication September 22, 2008.

Abstract: We report a previously undescribed quorum-sensing mechanism for triggering multicellularity in Bacillus subtilis. B. subtilis forms communities of cells known as biofilms in response to an unknown signal. We discovered that biofilm formation is stimulated by a variety of small molecules produced by bacteria—including the B. subtilis nonribosomal peptide surfactin—that share the ability to induce potassium leakage. Natural products that do not cause potassium leakage failed to induce multicellularity. Small-molecule-induced multicellularity was prevented by the addition of potassium, but not sodium or lithium. Evidence is presented that potassium leakage stimulates the activity of a membrane protein kinase, KinC, which governs the expression of genes involved in biofilm formation. We propose that KinC responds to lowered intracellular potassium concentration and that this is a quorum-sensing mechanism that enables B. subtilis to respond to related and unrelated bacteria.

Key Words: biofilm • quorum sensing


Author contributions: D.L., M.A.F., R.L., and R.K. designed research; D.L. and F.C. performed research; D.L., M.A.F., F.C., R.L., and R.K. analyzed data; and D.L., M.A.F., R.L., and R.K. wrote the paper.

The authors declare no conflict of interest.

This article contains supporting information online at www.pnas.org/cgi/content/full/0810940106/DCSupplemental.

* It is important to note that NCIB3610 is a wild strain that produces surfactin. The commonly used laboratory strain 168 does not produce surfactin because of a mutation in the sfp gene.

1To whom correspondence may be addressed. E-mail: losick{at}mcb.harvard.edu or rkolter{at}hms.harvard.edu

© 2009 by The National Academy of Sciences of the USA


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