Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

PNAS 108 (35): 14664-14669

Copyright © 2011 by the National Academy of Sciences.


BIOLOGICAL SCIENCES / PHYSIOLOGY

Control of body size by oxygen supply reveals size-dependent and size-independent mechanisms of molting and metamorphosis

Viviane Callier H. Frederik Nijhout1

Department of Biology, Duke University, Durham, NC 27708

Edited* by Mary Jane West-Eberhard, Smithsonian Tropical Research Institute, Ciudad Universitaria, Costa Rica, and approved July 28, 2011 (received for review April 27, 2011)

Abstract: Body size profoundly affects many aspects of animal biology, including metamorphosis, allometry, size-dependent alternative pathways of gene expression, and the social and ecological roles of individuals. However, regulation of body size is one of the fundamental unsolved problems in developmental biology. The control of body size requires a mechanism that assesses size and stops growth within a characteristic range of sizes. Under normal growth conditions in Manduca sexta, the endocrine cascade that causes the brain to initiate metamorphosis starts when the larva reaches a critical weight. Metamorphosis is initiated by a size-sensing mechanism, but the nature of this mechanism has remained elusive. Here we show that this size-sensing mechanism depends on the limited ability of a fixed tracheal system to sustain the oxygen supply to a growing individual. As body mass increases, the demand for oxygen also increases, but the fixed tracheal system does not allow a corresponding increase in oxygen supply. We show that interinstar molting has the same size-related oxygen-dependent mechanism of regulation as metamorphosis. We show that low oxygen tension induces molting at smaller body size, consistent with the hypothesis that under normal growth conditions, body size is regulated by a mechanism that senses oxygen limitation. We also found that under poor growth conditions, larvae may never attain the critical weight but eventually molt regardless. We show that under these conditions, larvae do not use the critical weight mechanism, but instead use a size-independent mechanism that is independent of the brain.


Freely available online through the PNAS open access option.

Author contributions: V.C. and H.F.N. designed research; V.C. performed research; V.C. and H.F.N. analyzed data; and V.C. and H.F.N. wrote the paper.

The authors declare no conflict of interest.

*This Direct Submission article had a prearranged editor.

1To whom correspondence should be addressed. E-mail: hfn{at}duke.edu.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mass and volume growth of an insect tracheal system within a single instar.
B. R. Helm and G. Davidowitz (2013)
J. Exp. Biol. 216, 4703-4711
   Abstract »    Full Text »    PDF »
The role of reduced oxygen in the developmental physiology of growth and metamorphosis initiation in Drosophila melanogaster.
V. Callier, A. W. Shingleton, C. S. Brent, S. M. Ghosh, J. Kim, and J. F. Harrison (2013)
J. Exp. Biol. 216, 4334-4340
   Abstract »    Full Text »    PDF »
The Temperature Size Rule in Arthropods: Independent of Macro-Environmental Variables but Size Dependent.
C. J. Klok and J. F. Harrison (2013)
Integr. Comp. Biol. 53, 557-570
   Abstract »    Full Text »    PDF »
A molt timer is involved in the metamorphic molt in Manduca sexta larvae.
Y. Suzuki, T. Koyama, K. Hiruma, L. M. Riddiford, and J. W. Truman (2013)
PNAS 110, 12518-12525
   Abstract »    Full Text »    PDF »
Hypoxia-induced compression in the tracheal system of the tobacco hornworm caterpillar, Manduca sexta.
K. J. Greenlee, J. J. Socha, H. B. Eubanks, P. Pedersen, W.-K. Lee, and S. D. Kirkton (2013)
J. Exp. Biol. 216, 2293-2301
   Abstract »    Full Text »    PDF »
Temperature-size rule is mediated by thermal plasticity of critical size in Drosophila melanogaster.
S. M. Ghosh, N. D. Testa, and A. W. Shingleton (2013)
Proc R Soc B 280, 20130174
   Abstract »    Full Text »    PDF »
Body size is not critical for critical PO2 in scarabaeid and tenebrionid beetles.
H. M. Lease, C. J. Klok, A. Kaiser, and J. F. Harrison (2012)
J. Exp. Biol. 215, 2524-2533
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882