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Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles
Christopher J. Roberts,1*
Bryce Nelson,2*
Matthew J. Marton,1
Roland Stoughton,1
Michael R. Meyer,1
Holly A. Bennett,1
Yudong
D. He,1
Hongyue Dai,1
Wynn L. Walker,1
Timothy R. Hughes,1
Mike Tyers,3
Charles Boone,2
Stephen H. Friend1
Genome-wide transcript profiling was used to monitor signal
transduction during yeast pheromone response. Genetic manipulationsallowed analysis of changes in gene expression underlying pheromonesignaling, cell cycle control, and polarized morphogenesis. Atwo-dimensional hierarchical clustered matrix, covering 383 ofthe most
highly regulated genes, was constructed from 46 diverseexperimental
conditions. Diagnostic subsets of coexpressed genesreflected signaling
activity, cross talk, and overlap of multiplemitogen-activated protein
kinase (MAPK) pathways. Analysis ofthe profiles specified by two
different MAPKs--Fus3p and Kss1p--revealedfunctional overlap of the
filamentous growth and mating responses.Global transcript analysis
reflects biological responses associatedwith the activation and
perturbation of signal transduction pathways.
1 Rosetta Inpharmatics, 12040 115th Avenue
Northeast, Kirkland, WA 98034, USA.
2 Biology
Department, Queen's University, Kingston, Ontario K7L 3N6, Canada.
3 Programme in Molecular Biology and Cancer, Samuel
Lunenfeld Research Institute, Room 1080, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario M5G 1X5, Canada.
*
These authors contributed equally to this work.
To whom correspondence should be addressed.
E-mail: boonec{at}biology.queensu.ca and sfriend{at}rii.com
The editors suggest the following Related Resources on Science sites:
In Science Signaling
EDITORS' CHOICE
(8 February 2000) Sci. STKE2000 (18), tw9.
[DOI: 10.1126/stke.2000.18.tw9] |Abstract »|Full Text »
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