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Science 296 (5571): 1319-1321

Copyright © 2002 by the American Association for the Advancement of Science

Induction and Suppression of RNA Silencing by an Animal Virus

Hongwei Li, Wan Xiang Li, Shou Wei Ding*

RNA silencing is a sequence-specific RNA degradation mechanism that is operational in plants and animals. Here, we show that flock house virus (FHV) is both an initiator and a target of RNA silencing in Drosophila host cells and that FHV infection requires suppression of RNA silencing by an FHV-encoded protein, B2. These findings establish RNA silencing as an adaptive antiviral defense in animal cells. B2 also inhibits RNA silencing in transgenic plants, providing evidence for a conserved RNA silencing pathway in the plant and animal kingdoms.

Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, CA 92521, USA.
*   To whom correspondence should be addressed. E-mail: shou-wei.ding{at}ucr.edu

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Inhibition of Epstein-Barr Virus (EBV) Reactivation by Short Interfering RNAs Targeting p38 Mitogen-Activated Protein Kinase or c-myc in EBV-Positive Epithelial Cells.
X. Gao, H. Wang, and T. Sairenji (2004)
J. Virol. 78, 11798-11806
   Abstract »    Full Text »    PDF »
Induction of Antiviral Immunity by Double-Stranded RNA in a Marine Invertebrate.
J. Robalino, C. L. Browdy, S. Prior, A. Metz, P. Parnell, P. Gross, and G. Warr (2004)
J. Virol. 78, 10442-10448
   Abstract »    Full Text »    PDF »
Effects of Length and Location on the Cellular Response to Double-Stranded RNA.
Q. Wang and G. G. Carmichael (2004)
Microbiol. Mol. Biol. Rev. 68, 432-452
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Differential Roles of AC2 and AC4 of Cassava Geminiviruses in Mediating Synergism and Suppression of Posttranscriptional Gene Silencing.
R. Vanitharani, P. Chellappan, J. S. Pita, and C. M. Fauquet (2004)
J. Virol. 78, 9487-9498
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Inhibition of Severe Acute Respiratory Syndrome Virus Replication by Small Interfering RNAs in Mammalian Cells.
Z. Wang, L. Ren, X. Zhao, T. Hung, A. Meng, J. Wang, and Y.-G. Chen (2004)
J. Virol. 78, 7523-7527
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Inhibition of influenza virus matrix (M1) protein expression and virus replication by U6 promoter-driven and lentivirus-mediated delivery of siRNA.
E. K.-W. Hui, E. M. Yap, D. S. An, I. S. Y. Chen, and D. P. Nayak (2004)
J. Gen. Virol. 85, 1877-1884
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Nodamura Virus Nonstructural Protein B2 Can Enhance Viral RNA Accumulation in both Mammalian and Insect Cells.
K. L. Johnson, B. D. Price, L. D. Eckerle, and L. A. Ball (2004)
J. Virol. 78, 6698-6704
   Abstract »    Full Text »    PDF »
The Plant Virus Tomato Spotted Wilt Tospovirus Activates the Immune System of Its Main Insect Vector, Frankliniella occidentalis.
R. B. Medeiros, R. d. O. Resende, and A. C. de Avila (2004)
J. Virol. 78, 4976-4982
   Abstract »    Full Text »    PDF »

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