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Science 296 (5574): 1873-1876

Copyright © 2002 by the American Association for the Advancement of Science

Dynamic Imaging of T Cell-Dendritic Cell Interactions in Lymph Nodes

Sabine Stoll,1 Jérôme Delon,1 Tilmann M. Brotz,2* Ronald N. Germain1dagger

T cell immune responses begin within organized lymphoid tissues. The pace, topology, and outcomes of the cellular interactions that underlie these responses have, so far, been inferred from static imaging of sectioned tissue or from studies of cultured cells. Here we report dynamic visualization of antigen-specific T cells interacting with dendritic cells within intact explanted lymph nodes. We observed immunological synapse formation and prolonged interactions between these two cell types, followed by the activation, dissociation, and rapid migration of T cells away from the antigenic stimulus. This high-resolution spatiotemporal analysis provides insight into the nature of cell interactions critical to early immune responses within lymphoid structures.

1 Lymphocyte Biology Section, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health,
2 Experimental Immunology Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
*   Present address: 270 Littlefield Avenue, South San Francisco, CA 94080, USA.

dagger    To whom correspondence should be addressed. E-mail: rgermain{at}

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J. Immunol. 170, 2862-2870
   Abstract »    Full Text »    PDF »
Autonomous T cell trafficking examined in vivo with intravital two-photon microscopy.
M. J. Miller, S. H. Wei, M. D. Cahalan, and I. Parker (2003)
PNAS 100, 2604-2609
   Abstract »    Full Text »    PDF »
Inducible Costimulatory Molecule-B7-Related Protein 1 Interactions Are Important for the Clonal Expansion and B Cell Helper Functions of Naive, Th1, and Th2 T Cells.
K. M. Smith, J. M. Brewer, P. Webb, A. J. Coyle, C. Gutierrez-Ramos, and P. Garside (2003)
J. Immunol. 170, 2310-2315
   Abstract »    Full Text »    PDF »

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