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Dynamic Imaging of T Cell-Dendritic Cell Interactions in Lymph Nodes
Sabine Stoll,1Jérôme Delon,1Tilmann M. Brotz,2*Ronald N. Germain1
T cell immune responses begin within organized lymphoid tissues.
The pace, topology, and outcomes of the cellular interactionsthat
underlie these responses have, so far, been inferred fromstatic
imaging of sectioned tissue or from studies of culturedcells. Here we
report dynamic visualization of antigen-specificT cells interacting
with dendritic cells within intact explantedlymph nodes. We observed
immunological synapse formation and prolongedinteractions between
these two cell types, followed by the activation,dissociation, and
rapid migration of T cells away from the antigenicstimulus. This
high-resolution spatiotemporal analysis providesinsight into the
nature of cell interactions critical to earlyimmune responses within
lymphoid structures.
1 Lymphocyte Biology Section, Laboratory of
Immunology, National Institute of Allergy and Infectious Diseases,
National Institutes of Health,
2 Experimental
Immunology Branch, National Cancer Institute, National Institutes of
Health, Bethesda, MD 20892, USA.
*
Present address: 270 Littlefield Avenue, South San Francisco, CA
94080, USA.
To whom correspondence should be addressed. E-mail:
rgermain{at}nih.gov
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