Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 296 (5576): 2232-2235

Copyright © 2002 by the American Association for the Advancement of Science

Disassembly of Transcriptional Regulatory Complexes by Molecular Chaperones

Brian C. Freeman,1 Keith R. Yamamoto2*

Many biological processes are initiated by cooperative assembly of large multicomponent complexes; however, mechanisms for modulating or terminating the actions of these complexes are not well understood. For example, hormone-bound intracellular receptors (IRs) nucleate formation of transcriptional regulatory complexes whose actions cease promptly upon hormone withdrawal. Here, we show that the p23 molecular chaperone localizes in vivo to genomic response elements in a hormone-dependent manner, disrupting receptor-mediated transcriptional activation in vivo and in vitro; Hsp90 weakly displayed similar activities. Indeed, p23 and Hsp90 also disrupted the activities of some non-IR-containing transcriptional regulatory complexes. We suggest that molecular chaperones promote disassembly of transcriptional regulatory complexes, thus enabling regulatory machineries to detect and respond to signaling changes.

1 Department of Cell and Structural Biology, University of Illinois, Urbana-Champaign, 601 South Goodwin Avenue, Urbana, IL 61801, USA.
2 Department of Cellular and Molecular Pharmacology, University of California, San Francisco, 513 Parnassus, San Francisco, CA 94143-0450, USA.
*   To whom correspondence should be addressed. E-mail: yamamoto{at}cgl.ucsf.edu



THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Protein aggregation can inhibit clathrin-mediated endocytosis by chaperone competition.
A. Yu, Y. Shibata, B. Shah, B. Calamini, D. C. Lo, and R. I. Morimoto (2014)
PNAS 111, E1481-E1490
   Abstract »    Full Text »    PDF »
Proteomic profiling and pathway analysis of the response of rat renal proximal convoluted tubules to metabolic acidosis.
K. L. Schauer, D. M. Freund, J. E. Prenni, and N. P. Curthoys (2013)
Am J Physiol Renal Physiol 305, F628-F640
   Abstract »    Full Text »    PDF »
Gedunin Inactivates the Co-chaperone p23 Protein Causing Cancer Cell Death by Apoptosis.
C. A. Patwardhan, A. Fauq, L. B. Peterson, C. Miller, B. S. J. Blagg, and A. Chadli (2013)
J. Biol. Chem. 288, 7313-7325
   Abstract »    Full Text »    PDF »
HSP-Molecular Chaperones in Cancer Biogenesis and Tumor Therapy: An Overview.
F. RAPPA, F. FARINA, G. ZUMMO, S. DAVID, C. CAMPANELLA, F. CARINI, G. TOMASELLO, P. DAMIANI, F. CAPPELLO, E. C. DE MACARIO, et al. (2012)
Anticancer Res 32, 5139-5150
   Abstract »    Full Text »    PDF »
Heat shock protein 90{alpha} (HSP90{alpha}), a substrate and chaperone of DNA-PK necessary for the apoptotic response.
S. Solier, K. W. Kohn, B. Scroggins, W. Xu, J. Trepel, L. Neckers, and Y. Pommier (2012)
PNAS 109, 12866-12872
   Abstract »    Full Text »    PDF »
Chaperoning of Mutant p53 Protein by Wild-type p53 Protein Causes Hypoxic Tumor Regression.
R. Gogna, E. Madan, P. Kuppusamy, and U. Pati (2012)
J. Biol. Chem. 287, 2907-2914
   Abstract »    Full Text »    PDF »
The myosin-binding UCS domain but not the Hsp90-binding TPR domain of the UNC-45 chaperone is essential for function in Caenorhabditis elegans.
W. Ni, A. H. Hutagalung, S. Li, and H. F. Epstein (2011)
J. Cell Sci. 124, 3164-3173
   Abstract »    Full Text »    PDF »
Control of the function of the transcription and repair factor TFIIH by the action of the cochaperone Ydj1.
M. Moriel-Carretero, C. Tous, and A. Aguilera (2011)
PNAS 108, 15300-15305
   Abstract »    Full Text »    PDF »
Coactivation of GR and NFKB alters the repertoire of their binding sites and target genes.
N. A. S. Rao, M. T. McCalman, P. Moulos, K.-J. Francoijs, A. Chatziioannou, F. N. Kolisis, M. N. Alexis, D. J. Mitsiou, and H. G. Stunnenberg (2011)
Genome Res. 21, 1404-1416
   Abstract »    Full Text »    PDF »
The Stress of Protein Misfolding: From Single Cells to Multicellular Organisms.
T. Gidalevitz, V. Prahlad, and R. I. Morimoto (2011)
Cold Spring Harb Perspect Biol 3, a009704
   Abstract »    Full Text »    PDF »
High Levels of Hsp90 Cochaperone p23 Promote Tumor Progression and Poor Prognosis in Breast Cancer by Increasing Lymph Node Metastases and Drug Resistance.
N. E. Simpson, W. M. Lambert, R. Watkins, S. Giashuddin, S. J. Huang, E. Oxelmark, R. Arju, T. Hochman, J. D. Goldberg, R. J. Schneider, et al. (2010)
Cancer Res. 70, 8446-8456
   Abstract »    Full Text »    PDF »
Global Targeting of Subcellular Heat Shock Protein-90 Networks for Therapy of Glioblastoma.
M. D. Siegelin, J. Plescia, C. M. Raskett, C. A. Gilbert, A. H. Ross, and D. C. Altieri (2010)
Mol. Cancer Ther. 9, 1638-1646
   Abstract »    Full Text »    PDF »
Hsp90 Directly Modulates the Spatial Distribution of AF9/MLLT3 and Affects Target Gene Expression.
J. J. Lin and C. S. Hemenway (2010)
J. Biol. Chem. 285, 11966-11973
   Abstract »    Full Text »    PDF »
FKBP51 Promotes Assembly of the Hsp90 Chaperone Complex and Regulates Androgen Receptor Signaling in Prostate Cancer Cells.
L. Ni, C.-S. Yang, D. Gioeli, H. Frierson, D. O. Toft, and B. M. Paschal (2010)
Mol. Cell. Biol. 30, 1243-1253
   Abstract »    Full Text »    PDF »
Cochaperone Activity of Human Butyrate-Induced Transcript 1 Facilitates Hepatitis C Virus Replication through an Hsp90-Dependent Pathway.
S. Taguwa, H. Kambara, H. Omori, H. Tani, T. Abe, Y. Mori, T. Suzuki, T. Yoshimori, K. Moriishi, and Y. Matsuura (2009)
J. Virol. 83, 10427-10436
   Abstract »    Full Text »    PDF »
Activation of Gene Transcription by Heat Shock Protein 27 May Contribute to Its Neuronal Protection.
M. J. Friedman, S. Li, and X.-J. Li (2009)
J. Biol. Chem. 284, 27944-27951
   Abstract »    Full Text »    PDF »
Cooperative Regulation of the Interferon Regulatory Factor-1 Tumor Suppressor Protein by Core Components of the Molecular Chaperone Machinery.
V. Narayan, M. Eckert, A. Zylicz, M. Zylicz, and K. L. Ball (2009)
J. Biol. Chem. 284, 25889-25899
   Abstract »    Full Text »    PDF »
Direct Interdomain Interactions Can Mediate Allosterism in the Thyroid Receptor.
B.-D. K. Putcha and E. J. Fernandez (2009)
J. Biol. Chem. 284, 22517-22524
   Abstract »    Full Text »    PDF »
Hsp90{beta} and p130cas: novel regulatory factors of MMP-13 expression in human osteoarthritic chondrocytes.
Z Fan, G Tardif, D Hum, N Duval, J-P Pelletier, and J Martel-Pelletier (2009)
Ann Rheum Dis 68, 976-982
   Abstract »    Full Text »    PDF »
The human CDK8 subcomplex is a molecular switch that controls Mediator coactivator function.
M. T. Knuesel, K. D. Meyer, C. Bernecky, and D. J. Taatjes (2009)
Genes & Dev. 23, 439-451
   Abstract »    Full Text »    PDF »
Trithorax requires Hsp90 for maintenance of active chromatin at sites of gene expression.
M. Tariq, U. Nussbaumer, Y. Chen, C. Beisel, and R. Paro (2009)
PNAS 106, 1157-1162
   Abstract »    Full Text »    PDF »
From hatching to dispatching: the multiple cellular roles of the Hsp70 molecular chaperone machinery.
E. Meimaridou, S. B Gooljar, and J P. Chapple (2009)
J. Mol. Endocrinol. 42, 1-9
   Abstract »    Full Text »    PDF »
Regulation of TATA-binding protein dynamics in living yeast cells.
R. O. Sprouse, T. S. Karpova, F. Mueller, A. Dasgupta, J. G. McNally, and D. T. Auble (2008)
PNAS 105, 13304-13308
   Abstract »    Full Text »    PDF »
Intra- and Intermonomer Interactions Are Required to Synergistically Facilitate ATP Hydrolysis in Hsp90.
C. N. Cunningham, K. A. Krukenberg, and D. A. Agard (2008)
J. Biol. Chem. 283, 21170-21178
   Abstract »    Full Text »    PDF »
Novel Steroid Receptor Phyto-Modulator Compound A Inhibits Growth and Survival of Prostate Cancer Cells.
A. Yemelyanov, J. Czwornog, L. Gera, S. Joshi, R. T. Chatterton Jr., and I. Budunova (2008)
Cancer Res. 68, 4763-4773
   Abstract »    Full Text »    PDF »
p23/Sba1p Protects against Hsp90 Inhibitors Independently of Its Intrinsic Chaperone Activity.
F. Forafonov, O. A. Toogun, I. Grad, E. Suslova, B. C. Freeman, and D. Picard (2008)
Mol. Cell. Biol. 28, 3446-3456
   Abstract »    Full Text »    PDF »
HSP90/70 chaperones are required for rapid nucleosome removal upon induction of the GAL genes of yeast.
M. Floer, G. O. Bryant, and M. Ptashne (2008)
PNAS 105, 2975-2980
   Abstract »    Full Text »    PDF »
The Heat Shock Protein 70 Cochaperone YDJ1 Is Required for Efficient Membrane-Specific Flock House Virus RNA Replication Complex Assembly and Function in Saccharomyces cerevisiae.
S. A. Weeks and D. J. Miller (2008)
J. Virol. 82, 2004-2012
   Abstract »    Full Text »    PDF »
Molecular chaperone Hsp90 stabilizes Pih1/Nop17 to maintain R2TP complex activity that regulates snoRNA accumulation.
R. Zhao, Y. Kakihara, A. Gribun, J. Huen, G. Yang, M. Khanna, M. Costanzo, R. L. Brost, C. Boone, T. R. Hughes, et al. (2008)
J. Cell Biol. 180, 563-578
   Abstract »    Full Text »    PDF »
Dynamics of the Hypoxia-inducible Factor-1-Vascular Endothelial Growth Factor Promoter Complex.
P. Yu and T. Kodadek (2007)
J. Biol. Chem. 282, 35035-35045
   Abstract »    Full Text »    PDF »
UXT is a novel and essential cofactor in the NF-{kappa}B transcriptional enhanceosome.
S. Sun, Y. Tang, X. Lou, L. Zhu, K. Yang, B. Zhang, H. Shi, and C. Wang (2007)
J. Cell Biol. 178, 231-244
   Abstract »    Full Text »    PDF »
cPGES/p23 Is Required for Glucocorticoid Receptor Function and Embryonic Growth but Not Prostaglandin E2 Synthesis.
A. K. Lovgren, M. Kovarova, and B. H. Koller (2007)
Mol. Cell. Biol. 27, 4416-4430
   Abstract »    Full Text »    PDF »
The p23 molecular chaperone promotes functional telomerase complexes through DNA dissociation.
O. A. Toogun, W. Zeiger, and B. C. Freeman (2007)
PNAS 104, 5765-5770
   Abstract »    Full Text »    PDF »
The Ligand Binding Domain Controls Glucocorticoid Receptor Dynamics Independent of Ligand Release.
S. H. Meijsing, C. Elbi, H. F. Luecke, G. L. Hager, and K. R. Yamamoto (2007)
Mol. Cell. Biol. 27, 2442-2451
   Abstract »    Full Text »    PDF »
Ligand-Specific Dynamics of the Androgen Receptor at Its Response Element in Living Cells.
T. I. Klokk, P. Kurys, C. Elbi, A. K. Nagaich, A. Hendarwanto, T. Slagsvold, C.-Y. Chang, G. L. Hager, and F. Saatcioglu (2007)
Mol. Cell. Biol. 27, 1823-1843
   Abstract »    Full Text »    PDF »
The Activity and Stability of the Transcriptional Coactivator p/CIP/SRC-3 Are Regulated by CARM1-Dependent Methylation.
H. Naeem, D. Cheng, Q. Zhao, C. Underhill, M. Tini, M. T. Bedford, and J. Torchia (2007)
Mol. Cell. Biol. 27, 120-134
   Abstract »    Full Text »    PDF »
The role of the proteasomal ATPases and activator monoubiquitylation in regulating Gal4 binding to promoters.
A. Ferdous, D. Sikder, T. Gillette, K. Nalley, T. Kodadek, and S. A. Johnston (2007)
Genes & Dev. 21, 112-123
   Abstract »    Full Text »    PDF »
Unliganded and hormone-bound glucocorticoid receptors interact with distinct hydrophobic sites in the Hsp90 C-terminal domain.
L. Fang, D. Ricketson, L. Getubig, and B. Darimont (2006)
PNAS 103, 18487-18492
   Abstract »    Full Text »    PDF »
Overview of Nomenclature of Nuclear Receptors.
P. Germain, B. Staels, C. Dacquet, M. Spedding, and V. Laudet (2006)
Pharmacol. Rev. 58, 685-704
   Abstract »    Full Text »    PDF »
The Hsp90 Cochaperone p23 Is Essential for Perinatal Survival.
I. Grad, T. A. McKee, S. M. Ludwig, G. W. Hoyle, P. Ruiz, W. Wurst, T. Floss, C. A. Miller III, and D. Picard (2006)
Mol. Cell. Biol. 26, 8976-8983
   Abstract »    Full Text »    PDF »
Signaling within a Coactivator Complex: Methylation of SRC-3/AIB1 Is a Molecular Switch for Complex Disassembly.
Q. Feng, P. Yi, J. Wong, and B. W. O'Malley (2006)
Mol. Cell. Biol. 26, 7846-7857
   Abstract »    Full Text »    PDF »
The Cochaperone p23 Differentially Regulates Estrogen Receptor Target Genes and Promotes Tumor Cell Adhesion and Invasion.
E. Oxelmark, J. M. Roth, P. C. Brooks, S. E. Braunstein, R. J. Schneider, and M. J. Garabedian (2006)
Mol. Cell. Biol. 26, 5205-5213
   Abstract »    Full Text »    PDF »
Genes invoked in the ovarian transition to menopause.
A. Zimon, A. Erat, T. V. Wald, B. Bissell, A. Koulova, C. H. Choi, D. Bachvarov, R. H. Reindollar, and A. Usheva (2006)
Nucleic Acids Res. 34, 3279-3287
   Abstract »    Full Text »    PDF »
GCUNC-45 Is a Novel Regulator for the Progesterone Receptor/hsp90 Chaperoning Pathway.
A. Chadli, J. D. Graham, M. G. Abel, T. A. Jackson, D. F. Gordon, W. M. Wood, S. J. Felts, K. B. Horwitz, and D. Toft (2006)
Mol. Cell. Biol. 26, 1722-1730
   Abstract »    Full Text »    PDF »
Transcription in four dimensions: nuclear receptor-directed initiation of gene expression.
R. Metivier, G. Reid, and F. Gannon (2006)
EMBO Rep. 7, 161-167
   Abstract »    Full Text »    PDF »
Identification of Candidate Regulators of Embryonic Stem Cell Differentiation by Comparative Phosphoprotein Affinity Profiling.
L. G. Puente, D. J. Borris, J.-F. Carriere, J. F. Kelly, and L. A. Megeney (2006)
Mol. Cell. Proteomics 5, 57-67
   Abstract »    Full Text »    PDF »
The DnaJ-Related Factor Mrj Interacts with Nuclear Factor of Activated T Cells c3 and Mediates Transcriptional Repression through Class II Histone Deacetylase Recruitment.
Y.-S. Dai, J. Xu, and J. D. Molkentin (2005)
Mol. Cell. Biol. 25, 9936-9948
   Abstract »    Full Text »    PDF »
Repression of Interleukin-5 Transcription by the Glucocorticoid Receptor Targets GATA3 Signaling and Involves Histone Deacetylase Recruitment.
Y.-K. Jee, J. Gilmour, A. Kelly, H. Bowen, D. Richards, C. Soh, P. Smith, C. Hawrylowicz, D. Cousins, T. Lee, et al. (2005)
J. Biol. Chem. 280, 23243-23250
   Abstract »    Full Text »    PDF »
Hsp90 and environmental impacts on epigenetic states: a model for the trans-generational effects of diethylstibesterol on uterine development and cancer.
D. M. Ruden, L. Xiao, M. D. Garfinkel, and X. Lu (2005)
Hum. Mol. Genet. 14, R149-R155
   Abstract »    Full Text »    PDF »
Ku Is a Novel Transcriptional Recycling Coactivator of the Androgen Receptor in Prostate Cancer Cells.
G. L. Mayeur, W.-J. Kung, A. Martinez, C. Izumiya, D. J. Chen, and H.-J. Kung (2005)
J. Biol. Chem. 280, 10827-10833
   Abstract »    Full Text »    PDF »
Ligand-Specific Dynamics of the Progesterone Receptor in Living Cells and during Chromatin Remodeling In Vitro.
G. V. Rayasam, C. Elbi, D. A. Walker, R. Wolford, T. M. Fletcher, D. P. Edwards, and G. L. Hager (2005)
Mol. Cell. Biol. 25, 2406-2418
   Abstract »    Full Text »    PDF »
Regulation of coactivator complex assembly and function by protein arginine methylation and demethylimination.
Y.-H. Lee, S. A. Coonrod, W. L. Kraus, M. A. Jelinek, and M. R. Stallcup (2005)
PNAS 102, 3611-3616
   Abstract »    Full Text »    PDF »
Effect of torsinA on membrane proteins reveals a loss of function and a dominant-negative phenotype of the dystonia-associated {Delta}E-torsinA mutant.
G. E. Torres, A. L. Sweeney, J.-M. Beaulieu, P. Shashidharan, and M. G. Caron (2004)
PNAS 101, 15650-15655
   Abstract »    Full Text »    PDF »
Dynamic Control of Nuclear Receptor Transcription.
A. M. Fowler and E. T. Alarid (2004)
Sci. STKE 2004, pe51
   Abstract »    Full Text »    PDF »
The Interaction between Sgt1p and Skp1p Is Regulated by HSP90 Chaperones and Is Required for Proper CBF3 Assembly.
L. B. Lingelbach and K. B. Kaplan (2004)
Mol. Cell. Biol. 24, 8938-8950
   Abstract »    Full Text »    PDF »
Attenuation of estrogen receptor {alpha}-mediated transcription through estrogen-stimulated recruitment of a negative elongation factor.
S. E. Aiyar, J.-l. Sun, A. L. Blair, C. A. Moskaluk, Y.-z. Lu, Q.-n. Ye, Y. Yamaguchi, A. Mukherjee, D.-m. Ren, H. Handa, et al. (2004)
Genes & Dev. 18, 2134-2146
   Abstract »    Full Text »    PDF »
Coactivator AIB1 links estrogen receptor transcriptional activity and stability.
W. Shao, E. K. Keeton, D. P. McDonnell, and M. Brown (2004)
PNAS 101, 11599-11604
   Abstract »    Full Text »    PDF »
Altered Hsp90 function in cancer: A unique therapeutic opportunity.
R. Bagatell and L. Whitesell (2004)
Mol. Cancer Ther. 3, 1021-1030
   Abstract »    Full Text »    PDF »
Global Nature of Dynamic Protein-Chromatin Interactions In Vivo: Three-Dimensional Genome Scanning and Dynamic Interaction Networks of Chromatin Proteins.
R. D. Phair, P. Scaffidi, C. Elbi, J. Vecerova, A. Dey, K. Ozato, D. T. Brown, G. Hager, M. Bustin, and T. Misteli (2004)
Mol. Cell. Biol. 24, 6393-6402
   Abstract »    Full Text »    PDF »
Progesterone Receptor Deficient in Chromatin Binding Has an Altered Cellular State.
J. Botos, W. Xian, D. F. Smith, and C. L. Smith (2004)
J. Biol. Chem. 279, 15231-15239
   Abstract »    Full Text »    PDF »
Rapid Glucocorticoid Receptor Exchange at a Promoter Is Coupled to Transcription and Regulated by Chaperones and Proteasomes.
D. A. Stavreva, W. G. Muller, G. L. Hager, C. L. Smith, and J. G. McNally (2004)
Mol. Cell. Biol. 24, 2682-2697
   Abstract »    Full Text »    PDF »
Molecular chaperones function as steroid receptor nuclear mobility factors.
C. Elbi, D. A. Walker, G. Romero, W. P. Sullivan, D. O. Toft, G. L. Hager, and D. B. DeFranco (2004)
PNAS 101, 2876-2881
   Abstract »    Full Text »    PDF »
Molecular determinants of responses to myocardial ischemia/reperfusion injury: focus on hypoxia-inducible and heat shock factors.
N. C Chi and J. S Karliner (2004)
Cardiovasc Res 61, 437-447
   Abstract »    Full Text »    PDF »
The Hsp90 Cochaperone p23 Is the Limiting Component of the Multiprotein Hsp90/Hsp70-based Chaperone System in Vivo Where It Acts to Stabilize the Client Protein{middle dot}Hsp90 Complex.
Y. Morishima, K. C. Kanelakis, P. J. M. Murphy, E. R. Lowe, G. J. Jenkins, Y. Osawa, R. K. Sunahara, and W. B. Pratt (2003)
J. Biol. Chem. 278, 48754-48763
   Abstract »    Full Text »    PDF »
GDP-Mannose 3',5'-Epimerase Forms GDP-L-gulose, a Putative Intermediate for the de Novo Biosynthesis of Vitamin C in Plants.
B. A. Wolucka and M. Van Montagu (2003)
J. Biol. Chem. 278, 47483-47490
   Abstract »    Full Text »    PDF »
Cytosolic HSP90 associates with and modulates the Arabidopsis RPM1 disease resistance protein.
D. A. Hubert, P. Tornero, Y. Belkhadir, P. Krishna, A. Takahashi, K. Shirasu, and J. L. Dangl (2003)
EMBO J. 22, 5679-5689
   Abstract »    Full Text »    PDF »
The Cochaperone Bag-1L Enhances Androgen Receptor Action via Interaction with the NH2-Terminal Region of the Receptor.
L. Shatkina, S. Mink, H. Rogatsch, H. Klocker, G. Langer, A. Nestl, and A. C. B. Cato (2003)
Mol. Cell. Biol. 23, 7189-7197
   Abstract »    Full Text »    PDF »
CHIP activates HSF1 and confers protection against apoptosis and cellular stress.
Q. Dai, C. Zhang, Y. Wu, H. McDonough, R. A. Whaley, V. Godfrey, H.-H. Li, N. Madamanchi, W. Xu, L. Neckers, et al. (2003)
EMBO J. 22, 5446-5458
   Abstract »    Full Text »    PDF »
Nuclear Export of the Glucocorticoid Receptor Is Accelerated by Cell Fusion-dependent Release of Calreticulin.
R. F. Walther, C. Lamprecht, A. Ridsdale, I. Groulx, S. Lee, Y. A. Lefebvre, and R. J. G. Hache (2003)
J. Biol. Chem. 278, 37858-37864
   Abstract »    Full Text »    PDF »
Genetic Dissection of p23, an Hsp90 Cochaperone, Reveals a Distinct Surface Involved in Estrogen Receptor Signaling.
E. Oxelmark, R. Knoblauch, S. Arnal, L. F. Su, M. Schapira, and M. J. Garabedian (2003)
J. Biol. Chem. 278, 36547-36555
   Abstract »    Full Text »    PDF »
Heat and Heavy Metal Stress Synergize to Mediate Transcriptional Hyperactivation by Metal-responsive Transcription Factor MTF-1.
N. Saydam, F. Steiner, O. Georgiev, and W. Schaffner (2003)
J. Biol. Chem. 278, 31879-31883
   Abstract »    Full Text »    PDF »
Functional Interaction of the DNA-binding Transcription Factor Sp1 through Its DNA-binding Domain with the Histone Chaperone TAF-I.
T. Suzuki, S. Muto, S. Miyamoto, K. Aizawa, M. Horikoshi, and R. Nagai (2003)
J. Biol. Chem. 278, 28758-28764
   Abstract »    Full Text »    PDF »
Cofactor Tpr2 combines two TPR domains and a J domain to regulate the Hsp70/Hsp90 chaperone system.
A. Brychzy, T. Rein, K. F. Winklhofer, F. U. Hartl, J. C. Young, and W. M. J. Obermann (2003)
EMBO J. 22, 3613-3623
   Abstract »    Full Text »    PDF »
Transcriptional activation of the NF-{kappa}B p65 subunit by mitogen- and stress-activated protein kinase-1 (MSK1).
L. Vermeulen, G. De Wilde, P. Van Damme, W. Vanden Berghe, and G. Haegeman (2003)
EMBO J. 22, 1313-1324
   Abstract »    Full Text »    PDF »
Essential Role of the Unusual DNA-binding Motif of BAG-1 for Inhibition of the Glucocorticoid Receptor.
U. Schmidt, G. M. Wochnik, M. C. Rosenhagen, J. C. Young, F. U. Hartl, F. Holsboer, and T. Rein (2003)
J. Biol. Chem. 278, 4926-4931
   Abstract »    Full Text »    PDF »
The Influence of ATP and p23 on the Conformation of hsp90.
W. P. Sullivan, B. A. L. Owen, and D. O. Toft (2002)
J. Biol. Chem. 277, 45942-45948
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882