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Science 297 (5580): 395-400

Copyright © 2002 by the American Association for the Advancement of Science

Systematic Identification of Pathways That Couple Cell Growth and Division in Yeast

Paul Jorgensen,12* Joy L. Nishikawa,12* Bobby-Joe Breitkreutz,2 Mike Tyers12dagger

Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of ~6000 Saccharomyces cerevisiae gene deletion strains and identified ~500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5. Ribosome biogenesis is intimately linked to cell size through Sfp1, a transcription factor that controls the expression of at least 60 genes implicated in ribosome assembly. Cell growth and division appear to be coupled by multiple conserved mechanisms.

1 Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
2 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: tyers{at}mshri.on.ca

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F. M. Roelants, P. D. Torrance, and J. Thorner (2004)
Microbiology 150, 3289-3304
   Abstract »    Full Text »    PDF »
Integrative Analysis of Cell Cycle Control in Budding Yeast.
K. C. Chen, L. Calzone, A. Csikasz-Nagy, F. R. Cross, B. Novak, and J. J. Tyson (2004)
Mol. Biol. Cell 15, 3841-3862
   Abstract »    Full Text »    PDF »
Reconstruction and Validation of Saccharomyces cerevisiae iND750, a Fully Compartmentalized Genome-Scale Metabolic Model.
N. C. Duarte, M. J. Herrgard, and B. O. Palsson (2004)
Genome Res. 14, 1298-1309
   Abstract »    Full Text »    PDF »
Control of cell size through phosphorylation of upstream binding factor 1 by nuclear phosphatidylinositol 3-kinase.
R. Drakas, X. Tu, and R. Baserga (2004)
PNAS 101, 9272-9276
   Abstract »    Full Text »    PDF »
Role of Pescadillo and Upstream Binding Factor in the Proliferation and Differentiation of Murine Myeloid Cells.
M. Prisco, A. Maiorana, C. Guerzoni, G. Calin, B. Calabretta, R. Voit, I. Grummt, and R. Baserga (2004)
Mol. Cell. Biol. 24, 5421-5433
   Abstract »    Full Text »    PDF »
A random-periods model for expression of cell-cycle genes.
D. Liu, D. M. Umbach, S. D. Peddada, L. Li, P. W. Crockett, and C. R. Weinberg (2004)
PNAS 101, 7240-7245
   Abstract »    Full Text »    PDF »
Cell Cycle Progression in G1 and S Phases Is CCR4 Dependent following Ionizing Radiation or Replication Stress in Saccharomyces cerevisiae.
T. J. Westmoreland, J. R. Marks, J. A. Olson Jr., E. M. Thompson, M. A. Resnick, and C. B. Bennett (2004)
Eukaryot. Cell 3, 430-446
   Abstract »    Full Text »    PDF »
A Synthetic Lethal Screen Identifies a Role for the Cortical Actin Patch/Endocytosis Complex in the Response to Nutrient Deprivation in Saccharomyces cerevisiae.
A. Care, K. A. Vousden, K. M. Binley, P. Radcliffe, J. Trevethick, I. Mannazzu, and P. E. Sudbery (2004)
Genetics 166, 707-719
   Abstract »    Full Text »    PDF »

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