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Science 297 (5580): 395-400

Copyright © 2002 by the American Association for the Advancement of Science

Systematic Identification of Pathways That Couple Cell Growth and Division in Yeast

Paul Jorgensen,12* Joy L. Nishikawa,12* Bobby-Joe Breitkreutz,2 Mike Tyers12dagger

Size homeostasis in budding yeast requires that cells grow to a critical size before commitment to division in the late prereplicative growth phase of the cell cycle, an event termed Start. We determined cell size distributions for the complete set of ~6000 Saccharomyces cerevisiae gene deletion strains and identified ~500 abnormally small (whi) or large (lge) mutants. Genetic analysis revealed a complex network of newly found factors that govern critical cell size at Start, the most potent of which were Sfp1, Sch9, Cdh1, Prs3, and Whi5. Ribosome biogenesis is intimately linked to cell size through Sfp1, a transcription factor that controls the expression of at least 60 genes implicated in ribosome assembly. Cell growth and division appear to be coupled by multiple conserved mechanisms.

1 Department of Medical Genetics and Microbiology, University of Toronto, Toronto, Ontario, Canada M5S 1A8.
2 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada M5G 1X5.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: tyers{at}mshri.on.ca



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Inhibitory phosphorylation of a mitotic cyclin-dependent kinase regulates the morphogenesis, cell size and virulence of the smut fungus Ustilago maydis.
C. Sgarlata and J. Perez-Martin (2005)
J. Cell Sci. 118, 3607-3622
   Abstract »    Full Text »    PDF »
Discovery, validation, and genetic dissection of transcription factor binding sites by comparative and functional genomics.
J. Gertz, L. Riles, P. Turnbaugh, S.-W. Ho, and B. A. Cohen (2005)
Genome Res. 15, 1145-1152
   Abstract »    Full Text »    PDF »
Data mining tools for the Saccharomyces cerevisiae morphological database.
T. L. Saito, J. Sese, Y. Nakatani, F. Sano, M. Yukawa, Y. Ohya, and S. Morishita (2005)
Nucleic Acids Res. 33, W753-W757
   Abstract »    Full Text »    PDF »
The Sphingoid Long Chain Base Phytosphingosine Activates AGC-type Protein Kinases in Saccharomyces cerevisiae Including Ypk1, Ypk2, and Sch9.
K. Liu, X. Zhang, R. L. Lester, and R. C. Dickson (2005)
J. Biol. Chem. 280, 22679-22687
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Dynamical Remodeling of the Transcriptome during Short-Term Anaerobiosis in Saccharomyces cerevisiae: Differential Response and Role of Msn2 and/or Msn4 and Other Factors in Galactose and Glucose Media.
L.-C. Lai, A. L. Kosorukoff, P. V. Burke, and K. E. Kwast (2005)
Mol. Cell. Biol. 25, 4075-4091
   Abstract »    Full Text »    PDF »
Regulation of Upstream Binding Factor 1 Activity by Insulin-like Growth Factor I Receptor Signaling.
A. Wu, X. Tu, M. Prisco, and R. Baserga (2005)
J. Biol. Chem. 280, 2863-2872
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Growth Rate and Cell Size Modulate the Synthesis of, and Requirement for, G1-Phase Cyclins at Start.
B. L. Schneider, J. Zhang, J. Markwardt, G. Tokiwa, T. Volpe, S. Honey, and B. Futcher (2004)
Mol. Cell. Biol. 24, 10802-10813
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