Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 297 (5590): 2267-2270

Copyright © 2002 by the American Association for the Advancement of Science

Role of Hec1 in Spindle Checkpoint Signaling and Kinetochore Recruitment of Mad1/Mad2

Silvia Martin-Lluesma, Volker M. Stucke, Erich A. Nigg*

The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells.

Department of Cell Biology, Max-Planck-Institute of Biochemistry, Am Klopferspitz 18a, D-82152 Martinsried, Germany.
*   To whom correspondence should be addressed. E-mail: nigg{at}biochem.mpg.de


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Pathway redundancy and protein essentiality revealed in the Saccharomyces cerevisiae interaction networks.
I. Ulitsky and R. Shamir (2014)
Mol Syst Biol 3, 104
   Abstract »    Full Text »    PDF »
A Bub1-Mad1 interaction targets the Mad1-Mad2 complex to unattached kinetochores to initiate the spindle checkpoint.
M. W. Moyle, T. Kim, N. Hattersley, J. Espeut, D. K. Cheerambathur, K. Oegema, and A. Desai (2014)
J. Cell Biol. 204, 647-657
   Abstract »    Full Text »    PDF »
Mad1 contribution to spindle assembly checkpoint signalling goes beyond presenting Mad2 at kinetochores.
S. Heinrich, K. Sewart, H. Windecker, M. Langegger, N. Schmidt, N. Hustedt, and S. Hauf (2014)
EMBO Rep. 15, 291-298
   Abstract »    Full Text »    PDF »
Mad1 kinetochore recruitment by Mps1-mediated phosphorylation of Bub1 signals the spindle checkpoint.
N. London and S. Biggins (2014)
Genes & Dev. 28, 140-152
   Abstract »    Full Text »    PDF »
Phosphorylation of Microtubule-binding Protein Hec1 by Mitotic Kinase Aurora B Specifies Spindle Checkpoint Kinase Mps1 Signaling at the Kinetochore.
T. Zhu, Z. Dou, B. Qin, C. Jin, X. Wang, L. Xu, Z. Wang, L. Zhu, F. Liu, X. Gao, et al. (2013)
J. Biol. Chem. 288, 36149-36159
   Abstract »    Full Text »    PDF »
Monopolar Spindle 1 (MPS1) Kinase Promotes Production of Closed MAD2 (C-MAD2) Conformer and Assembly of the Mitotic Checkpoint Complex.
A. R. Tipton, W. Ji, B. Sturt-Gillespie, M. E. Bekier II, K. Wang, W. R. Taylor, and S.-T. Liu (2013)
J. Biol. Chem. 288, 35149-35158
   Abstract »    Full Text »    PDF »
Complementary Interhelical Interactions between Three Buried Glu-Lys Pairs within Three Heptad Repeats Are Essential for Hec1-Nuf2 Heterodimerization and Mitotic Progression.
B. Ngo, C.-M. Hu, X. E. Guo, B. Ngo, R. Wei, J. Zhu, and W.-H. Lee (2013)
J. Biol. Chem. 288, 34403-34413
   Abstract »    Full Text »    PDF »
A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.
W. Nijenhuis, E. von Castelmur, D. Littler, V. De Marco, E. Tromer, M. Vleugel, M. H. J. van Osch, B. Snel, A. Perrakis, and G. J. P. L. Kops (2013)
J. Cell Biol. 201, 217-231
   Abstract »    Full Text »    PDF »
CENP-T provides a structural platform for outer kinetochore assembly.
T. Nishino, F. Rago, T. Hori, K. Tomii, I. M. Cheeseman, and T. Fukagawa (2013)
EMBO J. 32, 424-436
   Abstract »    Full Text »    PDF »
The KMN protein network - chief conductors of the kinetochore orchestra.
D. Varma and E. D. Salmon (2012)
J. Cell Sci. 125, 5927-5936
   Abstract »    Full Text »    PDF »
Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.
S. Heinrich, H. Windecker, N. Hustedt, and S. Hauf (2012)
J. Cell Sci. 125, 4720-4727
   Abstract »    Full Text »    PDF »
Human chromokinesins promote chromosome congression and spindle microtubule dynamics during mitosis.
C. Wandke, M. Barisic, R. Sigl, V. Rauch, F. Wolf, A. C. Amaro, C. H. Tan, A. J. Pereira, U. Kutay, H. Maiato, et al. (2012)
J. Cell Biol. 198, 847-863
   Abstract »    Full Text »    PDF »
CENP-E-dependent BubR1 autophosphorylation enhances chromosome alignment and the mitotic checkpoint.
Y. Guo, C. Kim, S. Ahmad, J. Zhang, and Y. Mao (2012)
J. Cell Biol. 198, 205-217
   Abstract »    Full Text »    PDF »
The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores.
G. Zhang, C. D. Kelstrup, X.-W. Hu, M. J. Kaas Hansen, M. R. Singleton, J. V. Olsen, and J. Nilsson (2012)
J. Cell Sci. 125, 3243-3253
   Abstract »    Full Text »    PDF »
Spindle checkpoint-independent inhibition of mitotic chromosome segregation by Drosophila Mps1.
F. Althoff, R. E. Karess, and C. F. Lehner (2012)
Mol. Biol. Cell 23, 2275-2291
   Abstract »    Full Text »    PDF »
Structure of human Mad1 C-terminal domain reveals its involvement in kinetochore targeting.
S. Kim, H. Sun, D. R. Tomchick, H. Yu, and X. Luo (2012)
PNAS 109, 6549-6554
   Abstract »    Full Text »    PDF »
RED, a Spindle Pole-associated Protein, Is Required for Kinetochore Localization of MAD1, Mitotic Progression, and Activation of the Spindle Assembly Checkpoint.
P.-C. Yeh, C.-C. Yeh, Y.-C. Chen, and Y.-L. Juang (2012)
J. Biol. Chem. 287, 11704-11716
   Abstract »    Full Text »    PDF »
Characterization of Spindle Checkpoint Kinase Mps1 Reveals Domain with Functional and Structural Similarities to Tetratricopeptide Repeat Motifs of Bub1 and BubR1 Checkpoint Kinases.
S. Lee, P. Thebault, L. Freschi, S. Beaufils, T. L. Blundell, C. R. Landry, V. M. Bolanos-Garcia, and S. Elowe (2012)
J. Biol. Chem. 287, 5988-6001
   Abstract »    Full Text »    PDF »
CENP-E Kinesin Interacts with SKAP Protein to Orchestrate Accurate Chromosome Segregation in Mitosis.
Y. Huang, W. Wang, P. Yao, X. Wang, X. Liu, X. Zhuang, F. Yan, J. Zhou, J. Du, T. Ward, et al. (2012)
J. Biol. Chem. 287, 1500-1509
   Abstract »    Full Text »    PDF »
Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.
D. Ito, Y. Saito, and T. Matsumoto (2012)
PNAS 109, 209-214
   Abstract »    Full Text »    PDF »
Spindle assembly checkpoint: the third decade.
A. Musacchio (2011)
Phil Trans R Soc B 366, 3595-3604
   Abstract »    Full Text »    PDF »
p31comet acts to ensure timely spindle checkpoint silencing subsequent to kinetochore attachment.
R. S. Hagan, M. S. Manak, H. K. Buch, M. G. Meier, P. Meraldi, J. V. Shah, P. K. Sorger, and S. J. Doxsey (2011)
Mol. Biol. Cell 22, 4236-4246
   Abstract »    Full Text »    PDF »
Phosphorylation of the Ndc80 complex protein, HEC1, by Nek2 kinase modulates chromosome alignment and signaling of the spindle assembly checkpoint.
R. Wei, B. Ngo, G. Wu, and W.-H. Lee (2011)
Mol. Biol. Cell 22, 3584-3594
   Abstract »    Full Text »    PDF »
Probing the in vivo function of Mad1:C-Mad2 in the spindle assembly checkpoint.
L. L. Fava, M. Kaulich, E. A. Nigg, and A. Santamaria (2011)
EMBO J. 30, 3322-3336
   Abstract »    Full Text »    PDF »
Shugoshin is a Mad1/Cdc20-like interactor of Mad2.
M. Orth, B. Mayer, K. Rehm, U. Rothweiler, D. Heidmann, T. A. Holak, and O. Stemmann (2011)
EMBO J. 30, 2868-2880
   Abstract »    Full Text »    PDF »
Physiological Relevance of Cell Cycle Kinases.
M. Malumbres (2011)
Physiol Rev 91, 973-1007
   Abstract »    Full Text »    PDF »
A mitotic role for Mad1 beyond the spindle checkpoint.
D. Emre, R. Terracol, A. Poncet, Z. Rahmani, and R. E. Karess (2011)
J. Cell Sci. 124, 1664-1671
   Abstract »    Full Text »    PDF »
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.
S. Santaguida, C. Vernieri, F. Villa, A. Ciliberto, and A. Musacchio (2011)
EMBO J. 30, 1508-1519
   Abstract »    Full Text »    PDF »
The Ndc80 complex uses a tripartite attachment point to couple microtubule depolymerization to chromosome movement.
J. G. Tooley, S. A. Miller, and P. T. Stukenberg (2011)
Mol. Biol. Cell 22, 1217-1226
   Abstract »    Full Text »    PDF »
The NDC80 complex proteins Nuf2 and Hec1 make distinct contributions to kinetochore-microtubule attachment in mitosis.
L. J. R. Sundin, G. J. Guimaraes, and J. G. DeLuca (2011)
Mol. Biol. Cell 22, 759-768
   Abstract »    Full Text »    PDF »
Temporal changes in Hec1 phosphorylation control kinetochore-microtubule attachment stability during mitosis.
K. F. DeLuca, S. M. A. Lens, and J. G. DeLuca (2011)
J. Cell Sci. 124, 622-634
   Abstract »    Full Text »    PDF »
CENP-U Cooperates with Hec1 to Orchestrate Kinetochore-Microtubule Attachment.
S. Hua, Z. Wang, K. Jiang, Y. Huang, T. Ward, L. Zhao, Z. Dou, and X. Yao (2011)
J. Biol. Chem. 286, 1627-1638
   Abstract »    Full Text »    PDF »
Drosophila Mis12 Complex Acts as a Single Functional Unit Essential for Anaphase Chromosome Movement and a Robust Spindle Assembly Checkpoint.
Z. Venkei, M. R. Przewloka, and D. M. Glover (2011)
Genetics 187, 131-140
   Abstract »    Full Text »    PDF »
CLASP1, astrin and Kif2b form a molecular switch that regulates kinetochore-microtubule dynamics to promote mitotic progression and fidelity.
A. L. Manning, S. F. Bakhoum, S. Maffini, C. Correia-Melo, H. Maiato, and D. A. Compton (2010)
EMBO J. 29, 3531-3543
   Abstract »    Full Text »    PDF »
Aurora B kinase controls the targeting of the Astrin-SKAP complex to bioriented kinetochores.
J. C. Schmidt, T. Kiyomitsu, T. Hori, C. B. Backer, T. Fukagawa, and I. M. Cheeseman (2010)
J. Cell Biol. 191, 269-280
   Abstract »    Full Text »    PDF »
Release of Mps1 from kinetochores is crucial for timely anaphase onset.
N. Jelluma, T. B. Dansen, T. Sliedrecht, N. P. Kwiatkowski, and G. J. P. L. Kops (2010)
J. Cell Biol. 191, 281-290
   Abstract »    Full Text »    PDF »
SPICE - a previously uncharacterized protein required for centriole duplication and mitotic chromosome congression.
M. Archinti, C. Lacasa, N. Teixido-Travesa, and J. Luders (2010)
J. Cell Sci. 123, 3039-3046
   Abstract »    Full Text »    PDF »
Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex.
L. Hewitt, A. Tighe, S. Santaguida, A. M. White, C. D. Jones, A. Musacchio, S. Green, and S. S. Taylor (2010)
J. Cell Biol. 190, 25-34
   Abstract »    Full Text »    PDF »
Spindly/CCDC99 Is Required for Efficient Chromosome Congression and Mitotic Checkpoint Regulation.
M. Barisic, B. Sohm, P. Mikolcevic, C. Wandke, V. Rauch, T. Ringer, M. Hess, G. Bonn, and S. Geley (2010)
Mol. Biol. Cell 21, 1968-1981
   Abstract »    Full Text »    PDF »
Welcome to a new kind of tension: translating kinetochore mechanics into a wait-anaphase signal.
T. J. Maresca and E. D. Salmon (2010)
J. Cell Sci. 123, 825-835
   Abstract »    Full Text »    PDF »
Hec1 Contributes to Mitotic Centrosomal Microtubule Growth for Proper Spindle Assembly through Interaction with Hice1.
G. Wu, R. Wei, E. Cheng, B. Ngo, and W.-H. Lee (2009)
Mol. Biol. Cell 20, 4686-4695
   Abstract »    Full Text »    PDF »
Elevating the frequency of chromosome mis-segregation as a strategy to kill tumor cells.
A. Janssen, G. J. P. L. Kops, and R. H. Medema (2009)
PNAS 106, 19108-19113
   Abstract »    Full Text »    PDF »
Enhanced genomic instabilities caused by deregulated microtubule dynamics and chromosome segregation: a perspective from genetic studies in mice.
C. V. Rao, H. Y. Yamada, Y. Yao, and W. Dai (2009)
Carcinogenesis 30, 1469-1474
   Abstract »    Full Text »    PDF »
A quantitative systems view of the spindle assembly checkpoint.
A. Ciliberto and J. V. Shah (2009)
EMBO J. 28, 2162-2173
   Abstract »    Full Text »    PDF »
Kinase Signaling in the Spindle Checkpoint.
J. Kang and H. Yu (2009)
J. Biol. Chem. 284, 15359-15363
   Abstract »    Full Text »    PDF »
Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.
Y. W. Chan, L. L. Fava, A. Uldschmid, M. H.A. Schmitz, D. W. Gerlich, E. A. Nigg, and A. Santamaria (2009)
J. Cell Biol. 185, 859-874
   Abstract »    Full Text »    PDF »
Spindle assembly checkpoint-related failure perturbs early embryonic divisions and reduces reproductive performance of LT/Sv mice.
Z. Maciejewska, Z. Polanski, K. Kisiel, J. Z Kubiak, and M. A Ciemerych (2009)
Reproduction 137, 931-942
   Abstract »    Full Text »    PDF »
Stable kinetochore-microtubule interactions depend on the Ska complex and its new component Ska3/C13Orf3.
T. N. Gaitanos, A. Santamaria, A. A. Jeyaprakash, B. Wang, E. Conti, and E. A. Nigg (2009)
EMBO J. 28, 1442-1452
   Abstract »    Full Text »    PDF »
Mimicking Ndc80 phosphorylation triggers spindle assembly checkpoint signalling.
S. Kemmler, M. Stach, M. Knapp, J. Ortiz, J. Pfannstiel, T. Ruppert, and J. Lechner (2009)
EMBO J. 28, 1099-1110
   Abstract »    Full Text »    PDF »
Dynein light intermediate chain 1 is required for progress through the spindle assembly checkpoint.
M. V. S. Sivaram, T. L. Wadzinski, S. D. Redick, T. Manna, and S. J. Doxsey (2009)
EMBO J. 28, 902-914
   Abstract »    Full Text »    PDF »
The nuclear export factor Xpo1p targets Mad1p to kinetochores in yeast.
R. J. Scott, L. V. Cairo, D. W. Van de Vosse, and R. W. Wozniak (2009)
J. Cell Biol. 184, 21-29
   Abstract »    Full Text »    PDF »
Regulation of Kinetochore Recruitment of Two Essential Mitotic Spindle Checkpoint Proteins by Mps1 Phosphorylation.
Q. Xu, S. Zhu, W. Wang, X. Zhang, W. Old, N. Ahn, and X. Liu (2009)
Mol. Biol. Cell 20, 10-20
   Abstract »    Full Text »    PDF »
Dynein, Lis1 and CLIP-170 counteract Eg5-dependent centrosome separation during bipolar spindle assembly.
M. E. Tanenbaum, L. Macurek, N. Galjart, and R. H. Medema (2008)
EMBO J. 27, 3235-3245
   Abstract »    Full Text »    PDF »
MCAK-Independent Functions of ch-Tog/XMAP215 in Microtubule Plus-End Dynamics.
A. R. Barr and F. Gergely (2008)
Mol. Cell. Biol. 28, 7199-7211
   Abstract »    Full Text »    PDF »
Hec1 overexpression hyperactivates the mitotic checkpoint and induces tumor formation in vivo.
E. Diaz-Rodriguez, R. Sotillo, J.-M. Schvartzman, and R. Benezra (2008)
PNAS 105, 16719-16724
   Abstract »    Full Text »    PDF »
Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores.
A. Tighe, O. Staples, and S. Taylor (2008)
J. Cell Biol. 181, 893-901
   Abstract »    Full Text »    PDF »
Small Molecule Targeting the Hec1/Nek2 Mitotic Pathway Suppresses Tumor Cell Growth in Culture and in Animal.
G. Wu, X.-L. Qiu, L. Zhou, J. Zhu, R. Chamberlin, J. Lau, P.-L. Chen, and W.-H. Lee (2008)
Cancer Res. 68, 8393-8399
   Abstract »    Full Text »    PDF »
Spindly attachments.
F. Civril and A. Musacchio (2008)
Genes & Dev. 22, 2302-2307
   Abstract »    Full Text »    PDF »
Dual Regulation of Mad2 Localization on Kinetochores by Bub1 and Dam1/DASH that Ensure Proper Spindle Interaction.
S. Saitoh, Y. Kobayashi, Y. Ogiyama, and K. Takahashi (2008)
Mol. Biol. Cell 19, 3885-3897
   Abstract »    Full Text »    PDF »
Distinct Sequence Elements of Cyclin B1 Promote Localization to Chromatin, Centrosomes, and Kinetochores during Mitosis.
A. M. Bentley, G. Normand, J. Hoyt, and R. W. King (2007)
Mol. Biol. Cell 18, 4847-4858
   Abstract »    Full Text »    PDF »
The Transforming Acidic Coiled Coil 3 Protein Is Essential for Spindle-dependent Chromosome Alignment and Mitotic Survival.
L. Schneider, F. Essmann, A. Kletke, P. Rio, H. Hanenberg, W. Wetzel, K. Schulze-Osthoff, B. Nurnberg, and R. P. Piekorz (2007)
J. Biol. Chem. 282, 29273-29283
   Abstract »    Full Text »    PDF »
Tension-sensitive Plk1 phosphorylation on BubR1 regulates the stability of kinetochore microtubule interactions.
S. Elowe, S. Hummer, A. Uldschmid, X. Li, and E. A. Nigg (2007)
Genes & Dev. 21, 2205-2219
   Abstract »    Full Text »    PDF »
BubR1 and APC/EB1 cooperate to maintain metaphase chromosome alignment.
J. Zhang, S. Ahmad, and Y. Mao (2007)
J. Cell Biol. 178, 773-784
   Abstract »    Full Text »    PDF »
Human NUF2 Interacts with Centromere-associated Protein E and Is Essential for a Stable Spindle Microtubule-Kinetochore Attachment.
D. Liu, X. Ding, J. Du, X. Cai, Y. Huang, T. Ward, A. Shaw, Y. Yang, R. Hu, C. Jin, et al. (2007)
J. Biol. Chem. 282, 21415-21424
   Abstract »    Full Text »    PDF »
BUB1 mediation of caspase-independent mitotic death determines cell fate.
Y. Niikura, A. Dixit, R. Scott, G. Perkins, and K. Kitagawa (2007)
J. Cell Biol. 178, 283-296
   Abstract »    Full Text »    PDF »
Polo-like Kinase 1 Facilitates Chromosome Alignment during Prometaphase through BubR1.
S. Matsumura, F. Toyoshima, and E. Nishida (2007)
J. Biol. Chem. 282, 15217-15227
   Abstract »    Full Text »    PDF »
The human Nup107-160 nuclear pore subcomplex contributes to proper kinetochore functions.
M. Zuccolo, A. Alves, V. Galy, S. Bolhy, E. Formstecher, V. Racine, J.-B. Sibarita, T. Fukagawa, R. Shiekhattar, T. Yen, et al. (2007)
EMBO J. 26, 1853-1864
   Abstract »    Full Text »    PDF »
Timely anaphase onset requires a novel spindle and kinetochore complex comprising Ska1 and Ska2.
A. Hanisch, H. H. Sillje, and E. A. Nigg (2006)
EMBO J. 25, 5504-5515
   Abstract »    Full Text »    PDF »
Activation of CDCA1-KNTC2, Members of Centromere Protein Complex, Involved in Pulmonary Carcinogenesis.
S. Hayama, Y. Daigo, T. Kato, N. Ishikawa, T. Yamabuki, M. Miyamoto, T. Ito, E. Tsuchiya, S. Kondo, and Y. Nakamura (2006)
Cancer Res. 66, 10339-10348
   Abstract »    Full Text »    PDF »
Mapping the assembly pathways that specify formation of the trilaminar kinetochore plates in human cells.
S.-T. Liu, J. B. Rattner, S. A. Jablonski, and T. J. Yen (2006)
J. Cell Biol. 175, 41-53
   Abstract »    Full Text »    PDF »
Induction of apoptosis by monastrol, an inhibitor of the mitotic kinesin Eg5, is independent of the spindle checkpoint..
G. M. Chin and R. Herbst (2006)
Mol. Cancer Ther. 5, 2580-2591
   Abstract »    Full Text »    PDF »
Loading of the 3F3/2 Antigen onto Kinetochores Is Dependent on the Ordered Assembly of the Spindle Checkpoint Proteins.
O. K. Wong and G. Fang (2006)
Mol. Biol. Cell 17, 4390-4399
   Abstract »    Full Text »    PDF »
The human kinetochore proteins Nnf1R and Mcm21R are required for accurate chromosome segregation.
A. D. McAinsh, P. Meraldi, V. M. Draviam, A. Toso, and P. K. Sorger (2006)
EMBO J. 25, 4033-4049
   Abstract »    Full Text »    PDF »
Phosphorylation- and Polo-Box-dependent Binding of Plk1 to Bub1 Is Required for the Kinetochore Localization of Plk1.
W. Qi, Z. Tang, and H. Yu (2006)
Mol. Biol. Cell 17, 3705-3716
   Abstract »    Full Text »    PDF »
Bmi-1 Is a Novel Molecular Marker of Nasopharyngeal Carcinoma Progression and Immortalizes Primary Human Nasopharyngeal Epithelial Cells..
L.-B. Song, M.-S. Zeng, W.-T. Liao, L. Zhang, H.-Y. Mo, W.-L. Liu, J.-Y. Shao, Q.-L. Wu, M.-Z. Li, Y.-F. Xia, et al. (2006)
Cancer Res. 66, 6225-6232
   Abstract »    Full Text »    PDF »
Coordinated Requirements of Human Topo II and Cohesin for Metaphase Centromere Alignment under Mad2-dependent Spindle Checkpoint Surveillance.
Y. Toyoda and M. Yanagida (2006)
Mol. Biol. Cell 17, 2287-2302
   Abstract »    Full Text »    PDF »
DNA Damage-induced Expression of p53 Suppresses Mitotic Checkpoint Kinase hMps1: THE LACK OF THIS SUPPRESSION IN p53MUT CELLS CONTRIBUTES TO APOPTOSIS.
M. R. Bhonde, M.-L. Hanski, J. Budczies, M. Cao, B. Gillissen, D. Moorthy, F. Simonetta, H. Scherubl, M. Truss, C. Hagemeier, et al. (2006)
J. Biol. Chem. 281, 8675-8685
   Abstract »    Full Text »    PDF »
Different Plk1 Functions Show Distinct Dependencies on Polo-Box Domain-mediated Targeting.
A. Hanisch, A. Wehner, E. A. Nigg, and H. H.W. Sillje (2006)
Mol. Biol. Cell 17, 448-459
   Abstract »    Full Text »    PDF »
Comparative Genomic Analyses of the Bacterial Phosphotransferase System.
R. D. Barabote and M. H. Saier Jr. (2005)
Microbiol. Mol. Biol. Rev. 69, 608-634
   Abstract »    Full Text »    PDF »
Unstable microtubule capture at kinetochores depleted of the centromere-associated protein CENP-F.
P. Bomont, P. Maddox, J. V. Shah, A. B. Desai, and D. W. Cleveland (2005)
EMBO J. 24, 3927-3939
   Abstract »    Full Text »    PDF »
Genome-wide transcriptional analysis of carboplatin response in chemosensitive and chemoresistant ovarian cancer cells.
D. Peters, J. Freund, and R. L. Ochs (2005)
Mol. Cancer Ther. 4, 1605-1616
   Abstract »    Full Text »    PDF »
Ablation of the spindle assembly checkpoint by a compound targeting Mps1.
M. Schmidt, Y. Budirahardja, R. Klompmaker, and R. H. Medema (2005)
EMBO Rep. 6, 866-872
   Abstract »    Full Text »    PDF »
Plx1 is the 3F3/2 kinase responsible for targeting spindle checkpoint proteins to kinetochores.
O. K. Wong and G. Fang (2005)
J. Cell Biol. 170, 709-719
   Abstract »    Full Text »    PDF »
Architecture of the Human Ndc80-Hec1 Complex, a Critical Constituent of the Outer Kinetochore.
C. Ciferri, J. De Luca, S. Monzani, K. J. Ferrari, D. Ristic, C. Wyman, H. Stark, J. Kilmartin, E. D. Salmon, and A. Musacchio (2005)
J. Biol. Chem. 280, 29088-29095
   Abstract »    Full Text »    PDF »
Spindle Checkpoint Signaling Requires the Mis6 Kinetochore Subcomplex, Which Interacts with Mad2 and Mitotic Spindles.
S. Saitoh, K. Ishii, Y. Kobayashi, and K. Takahashi (2005)
Mol. Biol. Cell 16, 3666-3677
   Abstract »    Full Text »    PDF »
The C-Terminal Half of Saccharomyces cerevisiae Mad1p Mediates Spindle Checkpoint Function, Chromosome Transmission Fidelity and CEN Association.
J. P. Kastenmayer, M. S. Lee, A. L. Hong, F. A. Spencer, and M. A. Basrai (2005)
Genetics 170, 509-517
   Abstract »    Full Text »    PDF »
CENP-A Is Required for Accurate Chromosome Segregation and Sustained Kinetochore Association of BubR1.
V. Regnier, P. Vagnarelli, T. Fukagawa, T. Zerjal, E. Burns, D. Trouche, W. Earnshaw, and W. Brown (2005)
Mol. Cell. Biol. 25, 3967-3981
   Abstract »    Full Text »    PDF »
Silencing Mitosin Induces Misaligned Chromosomes, Premature Chromosome Decondensation before Anaphase Onset, and Mitotic Cell Death.
Z. Yang, J. Guo, Q. Chen, C. Ding, J. Du, and X. Zhu (2005)
Mol. Cell. Biol. 25, 4062-4074
   Abstract »    Full Text »    PDF »
Vertebrate Nup53 Interacts with the Nuclear Lamina and Is Required for the Assembly of a Nup93-containing Complex.
L. A. Hawryluk-Gara, E. K. Shibuya, and R. W. Wozniak (2005)
Mol. Biol. Cell 16, 2382-2394
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882