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Science 298 (5591): 176-178

Copyright © 2002 by the American Association for the Advancement of Science

G Protein-Coupled Receptors in Anopheles gambiae

Catherine A. Hill,1* A. Nicole Fox,2* R. Jason Pitts,2* Lauren B. Kent,3 Perciliz L. Tan,1 Mathew A. Chrystal,1 Anibal Cravchik,4 Frank H. Collins,1 Hugh M. Robertson,3 Laurence J. Zwiebel2dagger

We used bioinformatic approaches to identify a total of 276 G protein-coupled receptors (GPCRs) from the Anopheles gambiae genome. These include GPCRs that are likely to play roles in pathways affecting almost every aspect of the mosquito's life cycle. Seventy-nine candidate odorant receptors were characterized for tissue expression and, along with 76 putative gustatory receptors, for their molecular evolution relative to Drosophila melanogaster. Examples of lineage-specific gene expansions were observed as well as a single instance of unusually high sequence conservation.

1 Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
2 Department of Biological Sciences, Program in Developmental Biology and Center for Molecular Neuroscience, Vanderbilt University, Nashville, TN 37235, USA.
3 Department of Entomology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA.
4 Celera Diagnostics, 45 West Gude Drive, Rockville, MD 20850-1232, USA.
*   These authors contributed equally to this work.

dagger    To whom all correspondence should be addressed. E-mail: l.zwiebel{at}

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