Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Subscribe

Logo for

Science 298 (5593): 608-611

Copyright © 2002 by the American Association for the Advancement of Science

Role of Predicted Metalloprotease Motif of Jab1/Csn5 in Cleavage of Nedd8 from Cul1

Gregory A. Cope,1 Greg S. B. Suh,3* L. Aravind,4 Sylvia E. Schwarz,1dagger S. Lawrence Zipursky,23 Eugene V. Koonin,4 Raymond J. Deshaies12ddagger

COP9 signalosome (CSN) cleaves the ubiquitin-like protein Nedd8 from the Cul1 subunit of SCF ubiquitin ligases. The Jab1/MPN domain metalloenzyme (JAMM) motif in the Jab1/Csn5 subunit was found to underlie CSN's Nedd8 isopeptidase activity. JAMM is found in proteins from archaea, bacteria, and eukaryotes, including the Rpn11 subunit of the 26S proteasome. Metal chelators and point mutations within JAMM abolished CSN-dependent cleavage of Nedd8 from Cul1, yet had little effect on CSN complex assembly. Optimal SCF activity in yeast and both viability and proper photoreceptor cell (R cell) development in Drosophila melanogaster required an intact Csn5 JAMM domain. We propose that JAMM isopeptidases play important roles in a variety of physiological pathways.

1 Department of Biology, California Institute of Technology (CalTech), Pasadena, CA 91125, USA.
2 Howard Hughes Medical Institute,
3 Department of Biological Chemistry, The School of Medicine, University of California at Los Angeles (UCLA), Los Angeles, CA 90095, USA.
4 National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.
*   Present address: Department of Biology, California Institute of Technology, Pasadena, CA 91125, USA.

dagger    Present address: G2M Cancer Drugs AG, Frankfurt/Main, Germany.

ddagger    To whom correspondence should be addressed. E-mail: deshaies{at}caltech.edu



THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dynamic Regulation of the COP9 Signalosome in Response to DNA Damage.
M. G. Fuzesi-Levi, G. Ben-Nissan, E. Bianchi, H. Zhou, M. J. Deery, K. S. Lilley, Y. Levin, and M. Sharon (2014)
Mol. Cell. Biol. 34, 1066-1076
   Abstract »    Full Text »    PDF »
A SnapShot of Ubiquitin Chain Elongation: LYSINE 48-TETRA-UBIQUITIN SLOWS DOWN UBIQUITINATION.
J. Kovacev, K. Wu, D. E. Spratt, R. A. Chong, C. Lee, J. Nayak, G. S. Shaw, and Z.-Q. Pan (2014)
J. Biol. Chem. 289, 7068-7081
   Abstract »    Full Text »    PDF »
CUL4A ubiquitin ligase: a promising drug target for cancer and other human diseases.
P. Sharma and A. Nag (2014)
Open Bio 4, 130217
   Abstract »    Full Text »    PDF »
Zinc-Dependent Interaction between JAB1 and Pre-S2 Mutant Large Surface Antigen of Hepatitis B Virus and Its Implications for Viral Hepatocarcinogenesis.
J.-L. Hsu, W.-J. Chuang, I.-J. Su, W.-J. Gui, Y.-Y. Chang, Y.-P. Lee, Y.-L. Ai, D. T. Chuang, and W. Huang (2013)
J. Virol. 87, 12675-12684
   Abstract »    Full Text »    PDF »
Building and remodelling Cullin-RING E3 ubiquitin ligases.
J. R. Lydeard, B. A. Schulman, and J. W. Harper (2013)
EMBO Rep. 14, 1050-1061
   Abstract »    Full Text »    PDF »
Jun Activation Domain-binding Protein 1 (JAB1) Is Required for the Optimal Response to Interferons.
R. Muromoto, M. Nakajima, K. Hirashima, T. Hirao, S. Kon, K. Shimoda, K. Oritani, and T. Matsuda (2013)
J. Biol. Chem. 288, 30969-30979
   Abstract »    Full Text »    PDF »
NEDD8 Ultimate Buster-1 Long (NUB1L) Protein Promotes Transfer of NEDD8 to Proteasome for Degradation through the P97UFD1/NPL4 Complex.
S. Liu, H. Yang, J. Zhao, Y.-H. Zhang, A.-X. Song, and H.-Y. Hu (2013)
J. Biol. Chem. 288, 31339-31349
   Abstract »    Full Text »    PDF »
The Arabidopsis COP9 SIGNALOSOME INTERACTING F-BOX KELCH 1 Protein Forms an SCF Ubiquitin Ligase and Regulates Hypocotyl Elongation.
A. Franciosini, B. Lombardi, S. Iafrate, V. Pecce, G. Mele, L. Lupacchini, G. Rinaldi, Y. Kondou, G. Gusmaroli, S. Aki, et al. (2013)
Mol Plant 6, 1616-1629
   Abstract »    Full Text »    PDF »
Crystal structure and versatile functional roles of the COP9 signalosome subunit 1.
J.-H. Lee, L. Yi, J. Li, K. Schweitzer, M. Borgmann, M. Naumann, and H. Wu (2013)
PNAS 110, 11845-11850
   Abstract »    Full Text »    PDF »
Deubiquitylases From Genes to Organism.
M. J. Clague, I. Barsukov, J. M. Coulson, H. Liu, D. J. Rigden, and S. Urbe (2013)
Physiol Rev 93, 1289-1315
   Abstract »    Full Text »    PDF »
The Deubiquitinating Enzyme AMSH1 and the ESCRT-III Subunit VPS2.1 Are Required for Autophagic Degradation in Arabidopsis.
A. Katsiarimpa, K. Kalinowska, F. Anzenberger, C. Weis, M. Ostertag, C. Tsutsumi, C. Schwechheimer, F. Brunner, R. Huckelhoven, and E. Isono (2013)
PLANT CELL 25, 2236-2252
   Abstract »    Full Text »    PDF »
The Cyclomodulin Cycle Inhibiting Factor (CIF) Alters Cullin Neddylation Dynamics.
T. B. Toro, J. I. Toth, and M. D. Petroski (2013)
J. Biol. Chem. 288, 14716-14726
   Abstract »    Full Text »    PDF »
incurvata13, a Novel Allele of AUXIN RESISTANT6, Reveals a Specific Role for Auxin and the SCF Complex in Arabidopsis Embryogenesis, Vascular Specification, and Leaf Flatness.
D. Esteve-Bruna, J. M. Perez-Perez, M. R. Ponce, and J. L. Micol (2013)
Plant Physiology 161, 1303-1320
   Abstract »    Full Text »    PDF »
Insights into the regulation of the human COP9 signalosome catalytic subunit, CSN5/Jab1.
A. Echalier, Y. Pan, M. Birol, N. Tavernier, L. Pintard, F. Hoh, C. Ebel, N. Galophe, F. X. Claret, and C. Dumas (2013)
PNAS 110, 1273-1278
   Abstract »    Full Text »    PDF »
UUCD: a family-based database of ubiquitin and ubiquitin-like conjugation.
T. Gao, Z. Liu, Y. Wang, H. Cheng, Q. Yang, A. Guo, J. Ren, and Y. Xue (2013)
Nucleic Acids Res. 41, D445-D451
   Abstract »    Full Text »    PDF »
The Organization of a CSN5-containing Subcomplex of the COP9 Signalosome.
G. G. Kotiguda, D. Weinberg, M. Dessau, C. Salvi, G. Serino, D. A. Chamovitz, and J. A. Hirsch (2012)
J. Biol. Chem. 287, 42031-42041
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome System of Saccharomyces cerevisiae.
D. Finley, H. D. Ulrich, T. Sommer, and P. Kaiser (2012)
Genetics 192, 319-360
   Abstract »    Full Text »    PDF »
The COP9 Signalosome: Its Regulation of Cullin-Based E3 Ubiquitin Ligases and Role in Photomorphogenesis.
C. D. Nezames and X. W. Deng (2012)
Plant Physiology 160, 38-46
   Full Text »    PDF »
Deconjugation of Nedd8 from Cul1 Is Directly Regulated by Skp1-F-box and Substrate, and the COP9 Signalosome Inhibits Deneddylated SCF by a Noncatalytic Mechanism.
E. D. Emberley, R. Mosadeghi, and R. J. Deshaies (2012)
J. Biol. Chem. 287, 29679-29689
   Abstract »    Full Text »    PDF »
The COP9 signalosome, cullin 3 and Keap1 supercomplex regulates CHOP stability and adipogenesis.
X. Huang, J. Ordemann, J. M. Muller, and W. Dubiel (2012)
Biology Open 1, 705-710
   Abstract »    Full Text »    PDF »
Mapping the Protein Interaction Network of the Human COP9 Signalosome Complex Using a Label-free QTAX Strategy.
L. Fang, R. M. Kaake, V. R. Patel, Y. Yang, P. Baldi, and L. Huang (2012)
Mol. Cell. Proteomics 11, 138-147
   Abstract »    Full Text »    PDF »
JAB1 Is Essential for B Cell Development and Germinal Center Formation and Inversely Regulates Fas Ligand and Bcl6 Expression.
S. Sitte, J. Glasner, J. Jellusova, F. Weisel, M. Panattoni, R. Pardi, and A. Gessner (2012)
J. Immunol. 188, 2677-2686
   Abstract »    Full Text »    PDF »
Posttranslational Modifications of Proteins in the Pathobiology of Medically Relevant Fungi.
M. D. Leach and A. J. P. Brown (2012)
Eukaryot. Cell 11, 98-108
   Abstract »    Full Text »    PDF »
Neddylation and CAND1 Independently Stimulate SCF Ubiquitin Ligase Activity in Candida albicans.
N. Sela, A. Atir-Lande, and D. Kornitzer (2012)
Eukaryot. Cell 11, 42-52
   Abstract »    Full Text »    PDF »
Characterization of the role of COP9 signalosome in regulating cullin E3 ubiquitin ligase activity.
Y. Y. Choo, B. K. Boh, J. J. W. Lou, J. Eng, Y. C. Leck, B. Anders, P. G. Smith, and T. Hagen (2011)
Mol. Biol. Cell 22, 4706-4715
   Abstract »    Full Text »    PDF »
MLN4924 Is an Efficient Inhibitor of NEDD8 Conjugation in Plants.
J. P. Hakenjos, R. Richter, E. M. N. Dohmann, A. Katsiarimpa, E. Isono, and C. Schwechheimer (2011)
Plant Physiology 156, 527-536
   Abstract »    Full Text »    PDF »
Insights into the evolution of Archaea and eukaryotic protein modifier systems revealed by the genome of a novel archaeal group.
T. Nunoura, Y. Takaki, J. Kakuta, S. Nishi, J. Sugahara, H. Kazama, G.-J. Chee, M. Hattori, A. Kanai, H. Atomi, et al. (2011)
Nucleic Acids Res. 39, 3204-3223
   Abstract »    Full Text »    PDF »
Dual function of Rpn5 in two PCI complexes, the 26S proteasome and COP9 signalosome.
Z. Yu, O. Kleifeld, A. Lande-Atir, M. Bsoul, M. Kleiman, D. Krutauz, A. Book, R. D. Vierstra, K. Hofmann, N. Reis, et al. (2011)
Mol. Biol. Cell 22, 911-920
   Abstract »    Full Text »    PDF »
CSN complex controls the stability of selected synaptic proteins via a torsinA-dependent process.
A. Granata, S. J. Koo, V. Haucke, G. Schiavo, and T. T. Warner (2011)
EMBO J. 30, 181-193
   Abstract »    Full Text »    PDF »
Recruitment of the inhibitor Cand1 to the cullin substrate adaptor site mediates interaction to the neddylation site.
K. Helmstaedt, E. U. Schwier, M. Christmann, K. Nahlik, M. Westermann, R. Harting, S. Grond, S. Busch, and G. H. Braus (2011)
Mol. Biol. Cell 22, 153-164
   Abstract »    Full Text »    PDF »
The Lys63-specific Deubiquitinating Enzyme BRCC36 Is Regulated by Two Scaffold Proteins Localizing in Different Subcellular Compartments.
L. Feng, J. Wang, and J. Chen (2010)
J. Biol. Chem. 285, 30982-30988
   Abstract »    Full Text »    PDF »
Ethylene-Induced Stabilization of ETHYLENE INSENSITIVE3 and EIN3-LIKE1 Is Mediated by Proteasomal Degradation of EIN3 Binding F-Box 1 and 2 That Requires EIN2 in Arabidopsis.
F. An, Q. Zhao, Y. Ji, W. Li, Z. Jiang, X. Yu, C. Zhang, Y. Han, W. He, Y. Liu, et al. (2010)
PLANT CELL 22, 2384-2401
   Abstract »    Full Text »    PDF »
The Human COP9 Signalosome Protects Ubiquitin-conjugating Enzyme 3 (UBC3/Cdc34) from {beta}-Transducin Repeat-containing Protein ({beta}TrCP)-mediated Degradation.
M. E. Fernandez-Sanchez, E. Sechet, F. Margottin-Goguet, L. Rogge, and E. Bianchi (2010)
J. Biol. Chem. 285, 17390-17397
   Abstract »    Full Text »    PDF »
DDB2 Complex-Mediated Ubiquitylation around DNA Damage Is Oppositely Regulated by XPC and Ku and Contributes to the Recruitment of XPA.
A. Takedachi, M. Saijo, and K. Tanaka (2010)
Mol. Cell. Biol. 30, 2708-2723
   Abstract »    Full Text »    PDF »
The Deubiquitinating Enzyme AMSH3 Is Required for Intracellular Trafficking and Vacuole Biogenesis in Arabidopsis thaliana.
E. Isono, A. Katsiarimpa, I. K. Muller, F. Anzenberger, Y.-D. Stierhof, N. Geldner, J. Chory, and C. Schwechheimer (2010)
PLANT CELL 22, 1826-1837
   Abstract »    Full Text »    PDF »
The TRC8 Ubiquitin Ligase Is Sterol Regulated and Interacts with Lipid and Protein Biosynthetic Pathways.
J. P. Lee, A. Brauweiler, M. Rudolph, J. E. Hooper, H. A. Drabkin, and R. M. Gemmill (2010)
Mol. Cancer Res. 8, 93-106
   Abstract »    Full Text »    PDF »
COP9 Signalosome Interacts ATP-dependently with p97/Valosin-containing Protein (VCP) and Controls the Ubiquitination Status of Proteins Bound to p97/VCP.
S. Cayli, J. Klug, J. Chapiro, S. Frohlich, G. Krasteva, L. Orel, and A. Meinhardt (2009)
J. Biol. Chem. 284, 34944-34953
   Abstract »    Full Text »    PDF »
The 26 S Proteasome: From Basic Mechanisms to Drug Targeting.
A. Navon and A. Ciechanover (2009)
J. Biol. Chem. 284, 33713-33718
   Abstract »    Full Text »    PDF »
A longevity protein, Lag2, interacts with SCF complex and regulates SCF function.
Y. Liu, S. Mimura, T. Kishi, and T. Kamura (2009)
EMBO J. 28, 3366-3377
   Abstract »    Full Text »    PDF »
Lessons from Fungal F-Box Proteins.
W. Jonkers and M. Rep (2009)
Eukaryot. Cell 8, 677-695
   Full Text »    PDF »
COP9 Signalosome- and 26S Proteasome-dependent Regulation of SCFTIR1 Accumulation in Arabidopsis.
J. Stuttmann, E. Lechner, R. Guerois, J. E. Parker, L. Nussaume, P. Genschik, and L. D. Noel (2009)
J. Biol. Chem. 284, 7920-7930
   Abstract »    Full Text »    PDF »
K63-specific deubiquitination by two JAMM/MPN+ complexes: BRISC-associated Brcc36 and proteasomal Poh1.
E. M. Cooper, C. Cutcliffe, T. Z. Kristiansen, A. Pandey, C. M. Pickart, and R. E. Cohen (2009)
EMBO J. 28, 621-631
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Is Required for Light-Dependent Timeless Degradation and Drosophila Clock Resetting.
A. Knowles, K. Koh, J.-T. Wu, C.-T. Chien, D. A. Chamovitz, and J. Blau (2009)
J. Neurosci. 29, 1152-1162
   Abstract »    Full Text »    PDF »
DEN1 deneddylates non-cullin proteins in vivo.
Y. Chan, J. Yoon, J.-T. Wu, H.-J. Kim, K.-T. Pan, J. Yim, and C.-T. Chien (2008)
J. Cell Sci. 121, 3218-3223
   Abstract »    Full Text »    PDF »
The Arabidopsis COP9 Signalosome Subunit 7 Is a Model PCI Domain Protein with Subdomains Involved in COP9 Signalosome Assembly.
M. Dessau, Y. Halimi, T. Erez, O. Chomsky-Hecht, D. A. Chamovitz, and J. A. Hirsch (2008)
PLANT CELL 20, 2815-2834
   Abstract »    Full Text »    PDF »
Auxin Responses in Mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome.
E. M. N. Dohmann, M. P. Levesque, E. Isono, M. Schmid, and C. Schwechheimer (2008)
Plant Physiology 147, 1369-1379
   Abstract »    Full Text »    PDF »
Genetic analysis of CAND1-CUL1 interactions in Arabidopsis supports a role for CAND1-mediated cycling of the SCFTIR1 complex.
W. Zhang, H. Ito, M. Quint, H. Huang, L. D. Noel, and W. M. Gray (2008)
PNAS 105, 8470-8475
   Abstract »    Full Text »    PDF »
The COP9/Signalosome Increases the Efficiency of von Hippel-Lindau Protein Ubiquitin Ligase-mediated Hypoxia-inducible Factor-{alpha} Ubiquitination.
Y. Miyauchi, M. Kato, F. Tokunaga, and K. Iwai (2008)
J. Biol. Chem. 283, 16622-16631
   Abstract »    Full Text »    PDF »
Targeted inactivation of the COP9 signalosome impairs multiple stagesof T cell development.
M. Panattoni, F. Sanvito, V. Basso, C. Doglioni, G. Casorati, E. Montini, J. R. Bender, A. Mondino, and R. Pardi (2008)
J. Exp. Med. 205, 465-477
   Abstract »    Full Text »    PDF »
CSN5 Isopeptidase Activity Links COP9 Signalosome Activation to Breast Cancer Progression.
A. S. Adler, L. E. Littlepage, M. Lin, T. L.A. Kawahara, D. J. Wong, Z. Werb, and H. Y. Chang (2008)
Cancer Res. 68, 506-515
   Abstract »    Full Text »    PDF »
CIF-1, a Shared Subunit of the COP9/Signalosome and Eukaryotic Initiation Factor 3 Complexes, Regulates MEL-26 Levels in the Caenorhabditis elegans Embryo.
S. Luke-Glaser, M. Roy, B. Larsen, T. Le Bihan, P. Metalnikov, M. Tyers, M. Peter, and L. Pintard (2007)
Mol. Cell. Biol. 27, 4526-4540
   Abstract »    Full Text »    PDF »
An eight-subunit COP9 signalosome with an intact JAMM motif is required for fungal fruit body formation.
S. Busch, E. U. Schwier, K. Nahlik, O. Bayram, K. Helmstaedt, O. W. Draht, S. Krappmann, O. Valerius, W. N. Lipscomb, and G. H. Braus (2007)
PNAS 104, 8089-8094
   Abstract »    Full Text »    PDF »
Ubiquitin, Hormones and Biotic Stress in Plants.
K. Dreher and J. Callis (2007)
Ann. Bot. 99, 787-822
   Abstract »    Full Text »    PDF »
The Subunit CSN6 of the COP9 Signalosome Is Cleaved during Apoptosis.
J. d. S. Correia, Y. Miranda, N. Leonard, and R. J. Ulevitch (2007)
J. Biol. Chem. 282, 12557-12565
   Abstract »    Full Text »    PDF »
Characterization of the VIER F-BOX PROTEINE Genes from Arabidopsis Reveals Their Importance for Plant Growth and Development.
K. M. Schwager, L. I. A. Calderon-Villalobos, E. M.N. Dohmann, B. C. Willige, S. Knierer, C. Nill, and C. Schwechheimer (2007)
PLANT CELL 19, 1163-1178
   Abstract »    Full Text »    PDF »
CSN controls NF-{kappa}B by deubiquitinylation of I{kappa}B{alpha}.
K. Schweitzer, P. M. Bozko, W. Dubiel, and M. Naumann (2007)
EMBO J. 26, 1532-1541
   Abstract »    Full Text »    PDF »
The Cullin3 Ubiquitin Ligase Functions as a Nedd8-bound Heterodimer.
W. Wimuttisuk and J. D. Singer (2007)
Mol. Biol. Cell 18, 899-909
   Abstract »    Full Text »    PDF »
The Assembly Pathway of the 19S Regulatory Particle of the Yeast 26S Proteasome.
E. Isono, K. Nishihara, Y. Saeki, H. Yashiroda, N. Kamata, L. Ge, T. Ueda, Y. Kikuchi, K. Tanaka, A. Nakano, et al. (2007)
Mol. Biol. Cell 18, 569-580
   Abstract »    Full Text »    PDF »
RIG-G as a key mediator of the antiproliferative activity of interferon-related pathways through enhancing p21 and p27 proteins.
S. Xiao, D. Li, H.-Q. Zhu, M.-G. Song, X.-R. Pan, P.-M. Jia, L.-L. Peng, A.-X. Dou, G.-Q. Chen, S.-J. Chen, et al. (2006)
PNAS 103, 16448-16453
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Regulates Skp2 Levels and Proliferation of Human Cells.
S. Denti, M. E. Fernandez-Sanchez, L. Rogge, and E. Bianchi (2006)
J. Biol. Chem. 281, 32188-32196
   Abstract »    Full Text »    PDF »
Jab1 Mediates Cytoplasmic Localization and Degradation of West Nile Virus Capsid Protein.
W. Oh, M.-R. Yang, E.-W. Lee, K.-m. Park, S. Pyo, J.-s. Yang, H.-W. Lee, and J. Song (2006)
J. Biol. Chem. 281, 30166-30174
   Abstract »    Full Text »    PDF »
SUMO-Conjugating and SUMO-Deconjugating Enzymes from Arabidopsis.
T. Colby, A. Matthai, A. Boeckelmann, and H.-P. Stuible (2006)
Plant Physiology 142, 318-332
   Abstract »    Full Text »    PDF »
Jab1 Induces the Cytoplasmic Localization and Degradation of p53 in Coordination with Hdm2.
W. Oh, E.-W. Lee, Y. H. Sung, M.-R. Yang, J. Ghim, H.-W. Lee, and J. Song (2006)
J. Biol. Chem. 281, 17457-17465
   Abstract »    Full Text »    PDF »
The Ubiquitin Isopeptidase UBPY Regulates Endosomal Ubiquitin Dynamics and Is Essential for Receptor Down-regulation.
P. E. Row, I. A. Prior, J. McCullough, M. J. Clague, and S. Urbe (2006)
J. Biol. Chem. 281, 12618-12624
   Abstract »    Full Text »    PDF »
Cul4A and DDB1 Associate with Skp2 To Target p27Kip1 for Proteolysis Involving the COP9 Signalosome.
T. Bondar, A. Kalinina, L. Khair, D. Kopanja, A. Nag, S. Bagchi, and P. Raychaudhuri (2006)
Mol. Cell. Biol. 26, 2531-2539
   Abstract »    Full Text »    PDF »
CAND1-Mediated Substrate Adaptor Recycling Is Required for Efficient Repression of Nrf2 by Keap1.
S.-C. Lo and M. Hannink (2006)
Mol. Cell. Biol. 26, 1235-1244
   Abstract »    Full Text »    PDF »
Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.
P. BELLARE, A. K. KUTACH, A. K. RINES, C. GUTHRIE, and E. J. SONTHEIMER (2006)
RNA 12, 292-302
   Abstract »    Full Text »    PDF »
Transactivation of Schizosaccharomyces pombe cdt2+ stimulates a Pcu4-Ddb1-CSN ubiquitin ligase.
C. Liu, M. Poitelea, A. Watson, S.-h. Yoshida, C. Shimoda, C. Holmberg, O. Nielsen, and A. M. Carr (2005)
EMBO J. 24, 3940-3951
   Abstract »    Full Text »    PDF »
The S100A7-c-Jun Activation Domain Binding Protein 1 Pathway Enhances Prosurvival Pathways in Breast Cancer.
E. D. Emberley, Y. Niu, L. Curtis, S. Troup, S. K. Mandal, J. N. Myers, S. B. Gibson, L. C. Murphy, and P. H. Watson (2005)
Cancer Res. 65, 5696-5702
   Abstract »    Full Text »    PDF »
Loss of the CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Subunit 5 Is Sufficient to Cause the cop/det/fus Mutant Phenotype in Arabidopsis.
E. M.N. Dohmann, C. Kuhnle, and C. Schwechheimer (2005)
PLANT CELL 17, 1967-1978
   Abstract »    Full Text »    PDF »
The COP9 signalosome regulates the Neurospora circadian clock by controlling the stability of the SCFFWD-1 complex.
Q. He, P. Cheng, Q. He, and Y. Liu (2005)
Genes & Dev. 19, 1518-1531
   Abstract »    Full Text »    PDF »
Myeloid leukemia factor 1 regulates p53 by suppressing COP1 via COP9 signalosome subunit 3.
N. Yoneda-Kato, K. Tomoda, M. Umehara, Y. Arata, and J.-y. Kato (2005)
EMBO J. 24, 1739-1749
   Abstract »    Full Text »    PDF »
Structural basis of NEDD8 ubiquitin discrimination by the deNEDDylating enzyme NEDP1.
L.-n. Shen, H. Liu, C. Dong, D. Xirodimas, J. H. Naismith, and R. T. Hay (2005)
EMBO J. 24, 1341-1351
   Abstract »    Full Text »    PDF »
The Jab1/COP9 signalosome subcomplex is a downstream mediator of Bcr-Abl kinase activity and facilitates cell-cycle progression.
K. Tomoda, J.-y. Kato, E. Tatsumi, T. Takahashi, Y. Matsuo, and N. Yoneda-Kato (2005)
Blood 105, 775-783
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Pathway and Plant Development.
J. Moon, G. Parry, and M. Estelle (2004)
PLANT CELL 16, 3181-3195
   Full Text »    PDF »
Translational Regulation via 5' mRNA Leader Sequences Revealed by Mutational Analysis of the Arabidopsis Translation Initiation Factor Subunit eIF3h.
T.-H. Kim, B.-H. Kim, A. Yahalom, D. A. Chamovitz, and A. G. von Arnim (2004)
PLANT CELL 16, 3341-3356
   Abstract »    Full Text »    PDF »
Multiple Functions of Jab1 Are Required for Early Embryonic Development and Growth Potential in Mice.
K. Tomoda, N. Yoneda-Kato, A. Fukumoto, S. Yamanaka, and J.-y. Kato (2004)
J. Biol. Chem. 279, 43013-43018
   Abstract »    Full Text »    PDF »
AMSH is an endosome-associated ubiquitin isopeptidase.
J. McCullough, M. J. Clague, and S. Urbe (2004)
J. Cell Biol. 166, 487-492
   Abstract »    Full Text »    PDF »
Crystal structure of human otubain 2.
M. H. Nanao, S. O. Tcherniuk, J. Chroboczek, O. Dideberg, A. Dessen, and M. Y. Balakirev (2004)
EMBO Rep. 5, 783-788
   Abstract »    Full Text »    PDF »
Interaction between Glucose-regulated Destruction Domain of DNA Topoisomerase II{alpha} and MPN Domain of Jab1/CSN5.
J. Yun, A. Tomida, T. Andoh, and T. Tsuruo (2004)
J. Biol. Chem. 279, 31296-31303
   Abstract »    Full Text »    PDF »
Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation.
S. Feng, Y. Shen, J. A. Sullivan, V. Rubio, Y. Xiong, T.-p. Sun, and X. W. Deng (2004)
PLANT CELL 16, 1870-1882
   Abstract »    Full Text »    PDF »
Arabidopsis ETA2, an Apparent Ortholog of the Human Cullin-Interacting Protein CAND1, Is Required for Auxin Responses Mediated by the SCFTIR1 Ubiquitin Ligase.
H.-w. Chuang, W. Zhang, and W. M. Gray (2004)
PLANT CELL 16, 1883-1897
   Abstract »    Full Text »    PDF »
A Genomic Analysis of Rat Proteases and Protease Inhibitors.
X. S. Puente and C. Lopez-Otin (2004)
Genome Res. 14, 609-622
   Abstract »    Full Text »    PDF »
Complementary Roles for Rpn11 and Ubp6 in Deubiquitination and Proteolysis by the Proteasome.
A. Guterman and M. H. Glickman (2004)
J. Biol. Chem. 279, 1729-1738
   Abstract »    Full Text »    PDF »
Specific and Covalent Targeting of Conjugating and Deconjugating Enzymes of Ubiquitin-Like Proteins.
J. Hemelaar, A. Borodovsky, B. M. Kessler, D. Reverter, J. Cook, N. Kolli, T. Gan-Erdene, K. D. Wilkinson, G. Gill, C. D. Lima, et al. (2004)
Mol. Cell. Biol. 24, 84-95
   Abstract »    Full Text »    PDF »
Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis.
J. T. Ohlmeyer and T. Schupbach (2003)
Development 130, 6339-6349
   Abstract »    Full Text »    PDF »
Disruption of the COP9 Signalosome Csn2 Subunit in Mice Causes Deficient Cell Proliferation, Accumulation of p53 and Cyclin E, and Early Embryonic Death.
K. Lykke-Andersen, L. Schaefer, S. Menon, X.-W. Deng, J. B. Miller, and N. Wei (2003)
Mol. Cell. Biol. 23, 6790-6797
   Abstract »    Full Text »    PDF »
COP9 Signalosome Subunit 3 Is Essential for Maintenance of Cell Proliferation in the Mouse Embryonic Epiblast.
J. Yan, K. Walz, H. Nakamura, S. Carattini-Rivera, Q. Zhao, H. Vogel, N. Wei, M. J. Justice, A. Bradley, and J. R. Lupski (2003)
Mol. Cell. Biol. 23, 6798-6808
   Abstract »    Full Text »    PDF »
Use of RNA Interference and Complementation To Study the Function of the Drosophila and Human 26S Proteasome Subunit S13.
J. Lundgren, P. Masson, C. A. Realini, and P. Young (2003)
Mol. Cell. Biol. 23, 5320-5330
   Abstract »    Full Text »    PDF »
DEN1 Is a Dual Function Protease Capable of Processing the C Terminus of Nedd8 and Deconjugating Hyper-neddylated CUL1.
K. Wu, K. Yamoah, G. Dolios, T. Gan-Erdene, P. Tan, A. Chen, C.-g. Lee, N. Wei, K. D. Wilkinson, R. Wang, et al. (2003)
J. Biol. Chem. 278, 28882-28891
   Abstract »    Full Text »    PDF »
Identification and Characterization of DEN1, a Deneddylase of the ULP Family.
T. Gan-Erdene, K. Nagamalleswari, L. Yin, K. Wu, Z.-Q. Pan, and K. D. Wilkinson (2003)
J. Biol. Chem. 278, 28892-28900
   Abstract »    Full Text »    PDF »
NEDP1, a Highly Conserved Cysteine Protease That deNEDDylates Cullins.
H. M. Mendoza, L.-n. Shen, C. Botting, A. Lewis, J. Chen, B. Ink, and R. T. Hay (2003)
J. Biol. Chem. 278, 25637-25643
   Abstract »    Full Text »    PDF »
A Novel Type of Deubiquitinating Enzyme.
P. C. Evans, T. S. Smith, M.-J. Lai, M. G. Williams, D. F. Burke, K. Heyninck**, M. M. Kreike, R. Beyaert, T. L. Blundell, and P. J. Kilshaw (2003)
J. Biol. Chem. 278, 23180-23186
   Abstract »    Full Text »    PDF »

To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882