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Science 298 (5602): 2388-2390

Copyright © 2002 by the American Association for the Advancement of Science

CREM-Dependent Transcription in Male Germ Cells Controlled by a Kinesin

Betina Macho,1 Stefano Brancorsini,1 Gian Maria Fimia,1 Mitsutoshi Setou,2 Nobutaka Hirokawa,2 Paolo Sassone-Corsi1*

ACT is a LIM-only protein expressed exclusively in round spermatids, where it cooperates with transcriptional activator CREM in regulating various postmeiotic genes. Targeted inactivation of CREM leads to a complete block of mouse spermiogenesis. We sought to identify the regulatory steps controlling the functional interplay between CREM and ACT. We found that ACT selectively associates with KIF17b, a kinesin highly expressed in male germ cells. The ACT-KIF17b interaction is restricted to specific stages of spermatogenesis and directly determines the intracellular localization of ACT. Sensitivity to leptomycin B indicates that KIF17b can be actively exported from the nucleus through the Crm1 receptor. Thus, a kinesin directly controls the activity of a transcriptional coactivator by a tight regulation of its intracellular localization.

1 Institut de Génétique et de Biologie Moléculaire et Cellulaire, B. P. 10142, 67404 Illkirch, Strasbourg, France.
2 Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
*   To whom correspondence should be addressed. E-mail: paolosc{at}igbmc.u-strasbg.fr



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