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Science 299 (5603): 114-116

Copyright © 2003 by the American Association for the Advancement of Science

Regulation of Chromatin Remodeling by Inositol Polyphosphates

David J. Steger,1 Elizabeth S. Haswell,1* Aimee L. Miller,2 Susan R. Wente,2 Erin K. O'Shea1dagger

Chromatin remodeling is required for efficient transcription of eukaryotic genes. In a genetic selection for budding yeast mutants that were defective in induction of the phosphate-responsive PHO5 gene, we identified mutations in ARG82/IPK2, which encodes a nuclear inositol polyphosphate kinase. In arg82 mutant strains, remodeling of PHO5 promoter chromatin is impaired, and the adenosine triphosphate-dependent chromatin-remodeling complexes SWI/SNF and INO80 are not efficiently recruited to phosphate-responsive promoters. These results suggest a role for the small molecule inositol polyphosphate in the regulation of chromatin remodeling and transcription.

1 Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA 94143-0448, USA.
2 Department of Cell and Developmental Biology, Vanderbilt University Medical Center, 3120A MRBIII, Nashville, TN 37212, USA.
*   Present address: Division of Biology, 156-29, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.

dagger    To whom correspondence should be addressed. E-mail: oshea{at}

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