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Science 299 (5610): 1228-1231

Copyright © 2003 by the American Association for the Advancement of Science

Proteomic Screen Finds pSer/pThr-Binding Domain Localizing Plk1 to Mitotic Substrates

Andrew E. H. Elia,12 Lewis C. Cantley,2 Michael B. Yaffe1*

We have developed a proteomic approach for identifying phosphopeptide binding domains that modulate kinase-dependent signaling pathways. An immobilized library of partially degenerate phosphopeptides biased toward a particular protein kinase phosphorylation motif is used to isolate phospho-binding domains that bind to proteins phosphorylated by that kinase. Applying this approach to cyclin-dependent kinases (Cdks), we identified the polo-box domain (PBD) of the mitotic kinase polo-like kinase 1 (Plk1) as a specific phosphoserine (pSer) or phosphothreonine (pThr) binding domain and determined its optimal binding motif. This motif is present in known Plk1 substrates such as Cdc25, and an optimal phosphopeptide containing the motif disrupted PBD-substrate binding and localization of the PBD to centrosomes. This finding reveals how Plk1 can localize to specific sites within cells in response to Cdk phosphorylation at those sites and provides a structural mechanism for targeting the Plk1 kinase domain to its substrates.

1 Center for Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
2 Division of Signal Transduction, Beth Israel Deaconess Hospital, and Department of Cell Biology, Harvard Medical School, Boston, MA 02215, USA.
*   To whom correspondence should be addressed. E-mail: myaffe{at}mit.edu

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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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A. Lindqvist, V. Rodriguez-Bravo, and R. H. Medema (2009)
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   Abstract »    Full Text »    PDF »
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T. J. Horner, W. S. Lai, D. J. Stumpo, and P. J. Blackshear (2009)
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   Abstract »    Full Text »    PDF »
Evidence for Regulation of Mitotic Progression through Temporal Phosphorylation and Dephosphorylation of CK2{alpha}.
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   Abstract »    Full Text »    PDF »
Regulated degradation of FANCM in the Fanconi anemia pathway during mitosis.
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   Abstract »    Full Text »    PDF »
Deficiency in Chromosome Congression by the Inhibition of Plk1 Polo Box Domain-dependent Recognition.
N. Watanabe, T. Sekine, M. Takagi, J.-i. Iwasaki, N. Imamoto, H. Kawasaki, and H. Osada (2009)
J. Biol. Chem. 284, 2344-2353
   Abstract »    Full Text »    PDF »
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L.-Y. Lu, J. L. Wood, K. Minter-Dykhouse, L. Ye, T. L. Saunders, X. Yu, and J. Chen (2008)
Mol. Cell. Biol. 28, 6870-6876
   Abstract »    Full Text »    PDF »
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J. Biol. Chem. 283, 28392-28400
   Abstract »    Full Text »    PDF »
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V. Archambault, P. P. D'Avino, M. J. Deery, K. S. Lilley, and D. M. Glover (2008)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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Role for Plk1 phosphorylation of Hbo1 in regulation of replication licensing.
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
PepCyber:P~PEP: a database of human protein protein interactions mediated by phosphoprotein-binding domains.
W. Gong, D. Zhou, Y. Ren, Y. Wang, Z. Zuo, Y. Shen, F. Xiao, Q. Zhu, A. Hong, X. Zhou, et al. (2008)
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   Abstract »    Full Text »    PDF »
Cell type dependent effects of Polo-like kinase 1 inhibition compared with targeted polo box interference in cancer cell lines.
J. Fink, K. Sanders, A. Rippl, S. Finkernagel, T. L. Beckers, and M. Schmidt (2007)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Mechanism of degradation of CPEB during Xenopus oocyte maturation.
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   Abstract »    Full Text »    PDF »
Use of the Novel Plk1 Inhibitor ZK-Thiazolidinone to Elucidate Functions of Plk1 in Early and Late Stages of Mitosis.
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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B. Garcia-Alvarez, G. de Carcer, S. Ibanez, E. Bragado-Nilsson, and G. Montoya (2007)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Polo-Like Kinase Cdc5 Controls the Local Activation of Rho1 to Promote Cytokinesis.
S. Yoshida, K. Kono, D. M. Lowery, S. Bartolini, M. B. Yaffe, Y. Ohya, and D. Pellman (2006)
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   Abstract »    Full Text »    PDF »
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N. Jiang, X. Wang, M. Jhanwar-Uniyal, Z. Darzynkiewicz, and W. Dai (2006)
J. Biol. Chem. 281, 10577-10582
   Abstract »    Full Text »    PDF »
Phosphoproteome analysis of the human mitotic spindle.
M. Nousiainen, H. H. W. Sillje, G. Sauer, E. A. Nigg, and R. Korner (2006)
PNAS 103, 5391-5396
   Abstract »    Full Text »    PDF »
CaMKII and Polo-like kinase 1 sequentially phosphorylate the cytostatic factor Emi2/XErp1 to trigger its destruction and meiotic exit.
D. V. Hansen, J. J. Tung, and P. K. Jackson (2006)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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