Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 299 (5612): 1572-1575

Copyright © 2003 by the American Association for the Advancement of Science

Role of Chloroplast Protein Kinase Stt7 in LHCII Phosphorylation and State Transition in Chlamydomonas

Nathalie Depège, Stéphane Bellafiore, Jean-David Rochaix*

Photosynthetic organisms adapt to changes in light quality by redistributing light excitation energy between two photosystems through state transition. This reorganization of antenna systems leads to an enhanced photosynthetic yield. Using a genetic approach in Chlamydomonas reinhardtii to dissect the signal transduction pathway of state transition, we identified a chloroplast thylakoid-associated serine-threonine protein kinase, Stt7, that has homologs in land plants. Stt7 is required for the phosphorylation of the major light-harvesting protein (LHCII) and for state transition.

Department of Molecular Biology and Department of Plant Biology, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva 4, Switzerland.
*   To whom correspondence should be addressed. E-mail: jean-david.rochaix{at}molbio.unige.ch

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A Dual Strategy to Cope with High Light in Chlamydomonas reinhardtii.
G. Allorent, R. Tokutsu, T. Roach, G. Peers, P. Cardol, J. Girard-Bascou, D. Seigneurin-Berny, D. Petroutsos, M. Kuntz, C. Breyton, et al. (2013)
PLANT CELL 25, 545-557
   Abstract »    Full Text »    PDF »
Protein kinases and phosphatases involved in the acclimation of the photosynthetic apparatus to a changing light environment.
J.-D. Rochaix, S. Lemeille, A. Shapiguzov, I. Samol, G. Fucile, A. Willig, and M. Goldschmidt-Clermont (2012)
Phil Trans R Soc B 367, 3466-3474
   Abstract »    Full Text »    PDF »
Identification of a Photosystem II Phosphatase Involved in Light Acclimation in Arabidopsis.
I. Samol, A. Shapiguzov, B. Ingelsson, G. Fucile, M. Crevecoeur, A. V. Vener, J.-D. Rochaix, and M. Goldschmidt-Clermont (2012)
PLANT CELL 24, 2596-2609
   Abstract »    Full Text »    PDF »
LHCBM1 and LHCBM2/7 Polypeptides, Components of Major LHCII Complex, Have Distinct Functional Roles in Photosynthetic Antenna System of Chlamydomonas reinhardtii.
P. Ferrante, M. Ballottari, G. Bonente, G. Giuliano, and R. Bassi (2012)
J. Biol. Chem. 287, 16276-16288
   Abstract »    Full Text »    PDF »
Three Acyltransferases and Nitrogen-responsive Regulator Are Implicated in Nitrogen Starvation-induced Triacylglycerol Accumulation in Chlamydomonas.
N. R. Boyle, M. D. Page, B. Liu, I. K. Blaby, D. Casero, J. Kropat, S. J. Cokus, A. Hong-Hermesdorf, J. Shaw, S. J. Karpowicz, et al. (2012)
J. Biol. Chem. 287, 15811-15825
   Abstract »    Full Text »    PDF »
Chloroplast-localized protein kinases: a step forward towards a complete inventory.
R. G. Bayer, S. Stael, A. G. Rocha, A. Mair, U. C. Vothknecht, and M. Teige (2012)
J. Exp. Bot. 63, 1713-1723
   Abstract »    Full Text »    PDF »
Plant organellar calcium signalling: an emerging field.
S. Stael, B. Wurzinger, A. Mair, N. Mehlmer, U. C. Vothknecht, and M. Teige (2012)
J. Exp. Bot. 63, 1525-1542
   Abstract »    Full Text »    PDF »
Evidence for a Role of VIPP1 in the Structural Organization of the Photosynthetic Apparatus in Chlamydomonas.
A. Nordhues, M. A. Schottler, A.-K. Unger, S. Geimer, S. Schonfelder, S. Schmollinger, M. Rutgers, G. Finazzi, B. Soppa, F. Sommer, et al. (2012)
PLANT CELL 24, 637-659
   Abstract »    Full Text »    PDF »
Overlapping Photoprotective Function of Vitamin E and Carotenoids in Chlamydomonas.
Z. Li, J. D. Keasling, and K. K. Niyogi (2012)
Plant Physiology 158, 313-323
   Abstract »    Full Text »    PDF »
Lumen Thiol Oxidoreductase1, a Disulfide Bond-Forming Catalyst, Is Required for the Assembly of Photosystem II in Arabidopsis.
M. Karamoko, S. Cline, K. Redding, N. Ruiz, and P. P. Hamel (2011)
PLANT CELL 23, 4462-4475
   Abstract »    Full Text »    PDF »
The Phosphorylation Status of the Chloroplast Protein Kinase STN7 of Arabidopsis Affects Its Turnover.
A. Willig, A. Shapiguzov, M. Goldschmidt-Clermont, and J.-D. Rochaix (2011)
Plant Physiology 157, 2102-2107
   Abstract »    Full Text »    PDF »
Control of Hydrogen Photoproduction by the Proton Gradient Generated by Cyclic Electron Flow in Chlamydomonas reinhardtii.
D. Tolleter, B. Ghysels, J. Alric, D. Petroutsos, I. Tolstygina, D. Krawietz, T. Happe, P. Auroy, J.-M. Adriano, A. Beyly, et al. (2011)
PLANT CELL 23, 2619-2630
   Abstract »    Full Text »    PDF »
A Forward Genetic Screen Identifies Mutants Deficient for Mitochondrial Complex I Assembly in Chlamydomonas reinhardtii.
M. R. Barbieri, V. Larosa, C. Nouet, N. Subrahmanian, C. Remacle, and P. P. Hamel (2011)
Genetics 188, 349-358
   Abstract »    Full Text »    PDF »
CCS5, a Thioredoxin-like Protein Involved in the Assembly of Plastid c-Type Cytochromes.
S. T. Gabilly, B. W. Dreyfuss, M. Karamoko, V. Corvest, J. Kropat, M. D. Page, S. S. Merchant, and P. P. Hamel (2010)
J. Biol. Chem. 285, 29738-29749
   Abstract »    Full Text »    PDF »
Stt7-dependent Phosphorylation during State Transitions in the Green Alga Chlamydomonas reinhardtii.
S. Lemeille, M. V. Turkina, A. V. Vener, and J.-D. Rochaix (2010)
Mol. Cell. Proteomics 9, 1281-1295
   Abstract »    Full Text »    PDF »
The PPH1 phosphatase is specifically involved in LHCII dephosphorylation and state transitions in Arabidopsis.
A. Shapiguzov, B. Ingelsson, I. Samol, C. Andres, F. Kessler, J.-D. Rochaix, A. V. Vener, and M. Goldschmidt-Clermont (2010)
PNAS 107, 4782-4787
   Abstract »    Full Text »    PDF »
Live-cell imaging of photosystem II antenna dissociation during state transitions.
M. Iwai, M. Yokono, N. Inada, and J. Minagawa (2010)
PNAS 107, 2337-2342
   Abstract »    Full Text »    PDF »
Thylakoid Protein Phosphorylation in Higher Plant Chloroplasts Optimizes Electron Transfer under Fluctuating Light.
M. Tikkanen, M. Grieco, S. Kangasjarvi, and E.-M. Aro (2010)
Plant Physiology 152, 723-735
   Abstract »    Full Text »    PDF »
The Chloroplast Kinase Network: New Insights from Large-Scale Phosphoproteome Profiling.
S. Baginsky and W. Gruissem (2009)
Mol Plant 2, 1141-1153
   Abstract »    Full Text »    PDF »
Impaired respiration discloses the physiological significance of state transitions in Chlamydomonas.
P. Cardol, J. Alric, J. Girard-Bascou, F. Franck, F.-A. Wollman, and G. Finazzi (2009)
PNAS 106, 15979-15984
   Abstract »    Full Text »    PDF »
Mechanism of REP27 Protein Action in the D1 Protein Turnover and Photosystem II Repair from Photodamage.
D. Dewez, S. Park, J. G. Garcia-Cerdan, P. Lindberg, and A. Melis (2009)
Plant Physiology 151, 88-99
   Abstract »    Full Text »    PDF »
Arabidopsis STN7 Kinase Provides a Link between Short- and Long-Term Photosynthetic Acclimation.
P. Pesaresi, A. Hertle, M. Pribil, T. Kleine, R. Wagner, H. Strissel, A. Ihnatowicz, V. Bonardi, M. Scharfenberg, A. Schneider, et al. (2009)
PLANT CELL 21, 2402-2423
   Abstract »    Full Text »    PDF »
The Role of Phosphorylation in Redox Regulation of Photosynthesis Genes psaA and psbA during Photosynthetic Acclimation of Mustard.
S. Steiner, L. Dietzel, Y. Schroter, V. Fey, R. Wagner, and T. Pfannschmidt (2009)
Mol Plant 2, 416-429
   Abstract »    Full Text »    PDF »
CP29, a Monomeric Light-harvesting Complex II Protein, Is Essential for State Transitions in Chlamydomonas reinhardtii.
R. Tokutsu, M. Iwai, and J. Minagawa (2009)
J. Biol. Chem. 284, 7777-7782
   Abstract »    Full Text »    PDF »
Characterization of Nda2, a Plastoquinone-reducing Type II NAD(P)H Dehydrogenase in Chlamydomonas Chloroplasts.
C. Desplats, F. Mus, S. Cuine, E. Billon, L. Cournac, and G. Peltier (2009)
J. Biol. Chem. 284, 4148-4157
   Abstract »    Full Text »    PDF »
Potential regulation of gene expression in photosynthetic cells by redox and energy state: approaches towards better understanding.
T. Pfannschmidt, K. Brautigam, R. Wagner, L. Dietzel, Y. Schroter, S. Steiner, and A. Nykytenko (2009)
Ann. Bot. 103, 599-607
   Abstract »    Full Text »    PDF »
A Novel Pathway of Cytochrome c Biogenesis Is Involved in the Assembly of the Cytochrome b6f Complex in Arabidopsis Chloroplasts.
L. Lezhneva, R. Kuras, G. Ephritikhine, and C. de Vitry (2008)
J. Biol. Chem. 283, 24608-24616
   Abstract »    Full Text »    PDF »
Molecular Remodeling of Photosystem II during State Transitions in Chlamydomonas reinhardtii.
M. Iwai, Y. Takahashi, and J. Minagawa (2008)
PLANT CELL 20, 2177-2189
   Abstract »    Full Text »    PDF »
A specific c-type cytochrome maturation system is required for oxygenic photosynthesis.
R. Kuras, D. Saint-Marcoux, F.-A. Wollman, and C. de Vitry (2007)
PNAS 104, 9906-9910
   Abstract »    Full Text »    PDF »
The Mobile Thylakoid Phosphoprotein TSP9 Interacts with the Light-harvesting Complex II and the Peripheries of Both Photosystems.
M. Hansson, T. Dupuis, R. Stromquist, B. Andersson, A. V. Vener, and I. Carlberg (2007)
J. Biol. Chem. 282, 16214-16222
   Abstract »    Full Text »    PDF »
The Membrane-Associated CpcG2-Phycobilisome in Synechocystis: A New Photosystem I Antenna.
K. Kondo, Y. Ochiai, M. Katayama, and M. Ikeuchi (2007)
Plant Physiology 144, 1200-1210
   Abstract »    Full Text »    PDF »
Loss of Phylloquinone in Chlamydomonas Affects Plastoquinone Pool Size and Photosystem II Synthesis.
L. Lefebvre-Legendre, F. Rappaport, G. Finazzi, M. Ceol, C. Grivet, G. Hopfgartner, and J.-D. Rochaix (2007)
J. Biol. Chem. 282, 13250-13263
   Abstract »    Full Text »    PDF »
REP27, a Tetratricopeptide Repeat Nuclear-Encoded and Chloroplast-Localized Protein, Functions in D1/32-kD Reaction Center Protein Turnover and Photosystem II Repair from Photodamage.
S. Park, P. Khamai, J. G. Garcia-Cerdan, and A. Melis (2007)
Plant Physiology 143, 1547-1560
   Abstract »    Full Text »    PDF »
Environmentally Modulated Phosphoproteome of Photosynthetic Membranes in the Green Alga Chlamydomonas reinhardtii.
M. V. Turkina, J. Kargul, A. Blanco-Rivero, A. Villarejo, J. Barber, and A. V. Vener (2006)
Mol. Cell. Proteomics 5, 1412-1425
   Abstract »    Full Text »    PDF »
Defects in the Cytochrome b6/f Complex Prevent Light-Induced Expression of Nuclear Genes Involved in Chlorophyll Biosynthesis.
N. Shao, O. Vallon, R. Dent, K. K. Niyogi, and C. F. Beck (2006)
Plant Physiology 141, 1128-1137
   Abstract »    Full Text »    PDF »
Pigment Shuffling in Antenna Systems Achieved by Expressing Prokaryotic Chlorophyllide a Oxygenase in Arabidopsis.
M. Hirashima, S. Satoh, R. Tanaka, and A. Tanaka (2006)
J. Biol. Chem. 281, 15385-15393
   Abstract »    Full Text »    PDF »
Identification of the mobile light-harvesting complex II polypeptides for state transitions in Chlamydomonas reinhardtii.
H. Takahashi, M. Iwai, Y. Takahashi, and J. Minagawa (2006)
PNAS 103, 477-482
   Abstract »    Full Text »    PDF »
NAB1 Is an RNA Binding Protein Involved in the Light-Regulated Differential Expression of the Light-Harvesting Antenna of Chlamydomonas reinhardtii.
J. H. Mussgnug, L. Wobbe, I. Elles, C. Claus, M. Hamilton, A. Fink, U. Kahmann, A. Kapazoglou, C. W. Mullineaux, M. Hippler, et al. (2005)
PLANT CELL 17, 3409-3421
   Abstract »    Full Text »    PDF »
Improved Photobiological H2 Production in Engineered Green Algal Cells.
O. Kruse, J. Rupprecht, K.-P. Bader, S. Thomas-Hall, P. M. Schenk, G. Finazzi, and B. Hankamer (2005)
J. Biol. Chem. 280, 34170-34177
   Abstract »    Full Text »    PDF »
Psb29, a Conserved 22-kD Protein, Functions in the Biogenesis of Photosystem II Complexes in Synechocystis and Arabidopsis.
N. Keren, H. Ohkawa, E. A. Welsh, M. Liberton, and H. B. Pakrasi (2005)
PLANT CELL 17, 2768-2781
   Abstract »    Full Text »    PDF »
STN8 Protein Kinase in Arabidopsis thaliana Is Specific in Phosphorylation of Photosystem II Core Proteins.
J. P. Vainonen, M. Hansson, and A. V. Vener (2005)
J. Biol. Chem. 280, 33679-33686
   Abstract »    Full Text »    PDF »
Isolation of phosphorylated and dephosphorylated forms of the CP43 internal antenna of photosystem II in Hordeum vulgare L..
F. Andreucci, R. Barbato, C. Picollo, and A. Segalla (2005)
J. Exp. Bot. 56, 1239-1244
   Abstract »    Full Text »    PDF »
The Light Reactions: A Guide to Recent Acquisitions for the Picture Gallery.
S. Merchant and M. R. Sawaya (2005)
PLANT CELL 17, 648-663
   Full Text »    PDF »
Functional Genomics of Eukaryotic Photosynthesis Using Insertional Mutagenesis of Chlamydomonas reinhardtii.
R. M. Dent, C. M. Haglund, B. L. Chin, M. C. Kobayashi, and K. K. Niyogi (2005)
Plant Physiology 137, 545-556
   Abstract »    Full Text »    PDF »
The central role of the green alga Chlamydomonas reinhardtii in revealing the mechanism of state transitions.
G. Finazzi (2005)
J. Exp. Bot. 56, 383-388
   Abstract »    Full Text »    PDF »
Plasticity in light reactions of photosynthesis for energy production and photoprotection.
J. A. Cruz, T. J. Avenson, A. Kanazawa, K. Takizawa, G. E. Edwards, and D. M. Kramer (2005)
J. Exp. Bot. 56, 395-406
   Abstract »    Full Text »    PDF »
Dynamics of photosystem II: a proteomic approach to thylakoid protein complexes.
E-M. Aro, M. Suorsa, A. Rokka, Y. Allahverdiyeva, V. Paakkarinen, A. Saleem, N. Battchikova, and E. Rintamaki (2005)
J. Exp. Bot. 56, 347-356
   Abstract »    Full Text »    PDF »
State transitions: an example of acclimation to low-light stress.
C. W. Mullineaux and D. Emlyn-Jones (2005)
J. Exp. Bot. 56, 389-393
   Abstract »    Full Text »    PDF »
Remodeling of Light-Harvesting Protein Complexes in Chlamydomonas in Response to Environmental Changes.
J. Nield, K. Redding, and M. Hippler (2004)
Eukaryot. Cell 3, 1370-1380
   Full Text »    PDF »
The Nucleus-encoded Protein MOC1 Is Essential for Mitochondrial Light Acclimation in Chlamydomonas reinhardtii.
C. Schonfeld, L. Wobbe, R. Borgstadt, A. Kienast, P. J. Nixon, and O. Kruse (2004)
J. Biol. Chem. 279, 50366-50374
   Abstract »    Full Text »    PDF »
ACCUMULATION OF PHOTOSYSTEM ONE1, a Member of a Novel Gene Family, Is Required for Accumulation of [4Fe-4S] Cluster-Containing Chloroplast Complexes and Antenna Proteins.
K. Amann, L. Lezhneva, G. Wanner, R. G. Herrmann, and J. Meurer (2004)
PLANT CELL 16, 3084-3097
   Abstract »    Full Text »    PDF »
Plastid Regulation of Lhcb1 Transcription in the Chlorophyte Alga Dunaliella tertiolecta.
Y.-B. Chen, D. G. Durnford, M. Koblizek, and P. G. Falkowski (2004)
Plant Physiology 136, 3737-3750
   Abstract »    Full Text »    PDF »
The Chloroplast Rieske Iron-Sulfur Protein: AT THE CROSSROAD OF ELECTRON TRANSPORT AND SIGNAL TRANSDUCTION.
C. de Vitry, Y. Ouyang, G. Finazzi, F.-A. Wollman, and T. Kallas (2004)
J. Biol. Chem. 279, 44621-44627
   Abstract »    Full Text »    PDF »
Chlamydomonas and Arabidopsis. A Dynamic Duo.
B. L. Gutman and K. K. Niyogi (2004)
Plant Physiology 135, 607-610
   Abstract »    Full Text »    PDF »
Modulation of energy-dependent quenching of excitons in antennae of higher plants.
T. J. Avenson, J. A. Cruz, and D. M. Kramer (2004)
PNAS 101, 5530-5535
   Abstract »    Full Text »    PDF »
In-Depth Analysis of the Thylakoid Membrane Proteome of Arabidopsis thaliana Chloroplasts: New Proteins, New Functions, and a Plastid Proteome Database.
G. Friso, L. Giacomelli, A. J. Ytterberg, J.-B. Peltier, A. Rudella, Q. Sun, and K. J. v. Wijk (2004)
PLANT CELL 16, 478-499
   Abstract »    Full Text »    PDF »
Light-harvesting Complex II Binds to Several Small Subunits of Photosystem I.
S. Zhang and H. V. Scheller (2004)
J. Biol. Chem. 279, 3180-3187
   Abstract »    Full Text »    PDF »
Rubisco Activase Is Required for Optimal Photosynthesis in the Green Alga Chlamydomonas reinhardtii in a Low-CO2 Atmosphere.
S. V. Pollock, S. L. Colombo, D. L. Prout Jr., A. C. Godfrey, and J. V. Moroney (2003)
Plant Physiology 133, 1854-1861
   Abstract »    Full Text »
Proteomics of Chlamydomonas reinhardtii Light-Harvesting Proteins.
E. J. Stauber, A. Fink, C. Markert, O. Kruse, U. Johanningmeier, and M. Hippler (2003)
Eukaryot. Cell 2, 978-994
   Abstract »    Full Text »    PDF »
Dithiol Oxidant and Disulfide Reductant Dynamically Regulate the Phosphorylation of Light-Harvesting Complex II Proteins in Thylakoid Membranes.
P. Martinsuo, S. Pursiheimo, E.-M. Aro, and E. Rintamaki (2003)
Plant Physiology 133, 37-46
   Abstract »    Full Text »    PDF »
Identification of Three Previously Unknown in Vivo Protein Phosphorylation Sites in Thylakoid Membranes of Arabidopsis thaliana.
M. Hansson and A. V. Vener (2003)
Mol. Cell. Proteomics 2, 550-559
   Abstract »    Full Text »    PDF »
BOTANY: State Transitions--a Question of Balance.
J. F. Allen (2003)
Science 299, 1530-1532
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882