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Loss of a Callose Synthase Results in Salicylic Acid-Dependent Disease Resistance
Marc T. Nishimura,1*
Mónica Stein,1*
Bi-Huei Hou,1
John P. Vogel,1
Herb Edwards,2
Shauna C. Somerville1
Abstract:
Plants attacked by pathogens rapidly deposit callose, a ß-1,3-glucan,at wound sites. Traditionally, this deposition is thought toreinforce the cell wall and is regarded as a defense response.Surprisingly, here we found that powdery mildew resistant 4(pmr4), a mutant lacking pathogen-induced callose, became resistantto pathogens, rather than more susceptible. This resistancewas due to mutation of a callose synthase, resulting in a lossof the induced callose response. Double-mutant analysis indicatedthat blocking the salicylic acid (SA) defense signaling pathwaywas sufficient to restore susceptibility to pmr4 mutants. Thus,callose or callose synthase negatively regulates the SA pathway.
1 Department of Plant Biology, Carnegie Institution, Stanford, CA 94305, USA. 2 Department of Biological Sciences, Western Illinois University, Macomb, IL 61455, USA.
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142, 561-570
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W. Talloen, D.-A. Clevert, S. Hochreiter, D. Amaratunga, L. Bijnens, S. Kass, and H. W.H. Gohlmann (2007)
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|Abstract »|Full Text »|PDF »
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T. Vellosillo, M. Martinez, M. A. Lopez, J. Vicente, T. Cascon, L. Dolan, M. Hamberg, and C. Castresana (2007)
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|Abstract »|Full Text »|PDF »
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G. Liu, D. L. Greenshields, R. Sammynaiken, R. N. Hirji, G. Selvaraj, and Y. Wei (2007)
J. Cell Sci.
120, 596-605
|Abstract »|Full Text »|PDF »
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143, 784-800
|Abstract »|Full Text »|PDF »
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|Abstract »|Full Text »|PDF »
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S. Bosca, C. J. Barton, N. G. Taylor, P. Ryden, L. Neumetzler, M. Pauly, K. Roberts, and G. J. Seifert (2006)
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142, 1353-1363
|Abstract »|Full Text »|PDF »
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|Abstract »|Full Text »|PDF »
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M. Stein, J. Dittgen, C. Sanchez-Rodriguez, B.-H. Hou, A. Molina, P. Schulze-Lefert, V. Lipka, and S. Somerville (2006)
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|Abstract »|Full Text »|PDF »
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|Abstract »|Full Text »|PDF »
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139, 1890-1901
|Abstract »|Full Text »|PDF »
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J. Ton, G. Jakab, V. Toquin, V. Flors, A. Iavicoli, M. N. Maeder, J.-P. Metraux, and B. Mauch-Mani (2005)
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17, 987-999
|Abstract »|Full Text »|PDF »
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J. P. Anderson, E. Badruzsaufari, P. M. Schenk, J. M. Manners, O. J. Desmond, C. Ehlert, D. J. Maclean, P. R. Ebert, and K. Kazan (2004)
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16, 3460-3479
|Abstract »|Full Text »|PDF »
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R. Menard, S. Alban, P. de Ruffray, F. Jamois, G. Franz, B. Fritig, J.-C. Yvin, and S. Kauffmann (2004)
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16, 3020-3032
|Abstract »|Full Text »|PDF »
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F. F. Assaad, J.-L. Qiu, H. Youngs, D. Ehrhardt, L. Zimmerli, M. Kalde, G. Wanner, S. C. Peck, H. Edwards, K. Ramonell, et al. (2004)
Mol. Biol. Cell
15, 5118-5129
|Abstract »|Full Text »|PDF »
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D. Hofius, M.-R. Hajirezaei, M. Geiger, H. Tschiersch, M. Melzer, and U. Sonnewald (2004)
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135, 1256-1268
|Abstract »|Full Text »|PDF »
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Shauna C. Somerville1
Present address: Western Regional Research Center, U.S. Department of Agriculture (USDA), Albany, CA 94710, USA. 
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