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Science 301 (5636): 1107-1110

Copyright © 2003 by the American Association for the Advancement of Science

SIR1, an Upstream Component in Auxin Signaling Identified by Chemical Genetics

Yunde Zhao,1* Xinhua Dai,1 Helen E. Blackwell,2{dagger} Stuart L. Schreiber,2 Joanne Chory3

Abstract: Auxin is a plant hormone that regulates many aspects of plant growth and development. We used a chemical genetics approach to identify SIR1, a regulator of many auxin-inducible genes. The sir1 mutant was resistant to sirtinol, a small molecule that activates many auxin-inducible genes and promotes auxin-related developmental phenotypes. SIR1 is predicted to encode a protein composed of a ubiquitin-activating enzyme E1–like domain and a Rhodanese-like domain homologous to that of prolyl isomerase. We suggest a molecular context for how the auxin signal is propagated to exert its biological effects.

1 Section of Cell and Developmental Biology, Division of Biological Sciences, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093–0116, USA.
2 Department of Chemistry and Chemical Biology, Howard Hughes Medical Institute (HHMI), Harvard University, 12 Oxford Street, Cambridge, MA 02138, USA.
3 The Plant Biology Laboratory, HHMI and The Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.

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{dagger} Present address: Department of Chemistry, University of Wisconsin–Madison, 1101 University Avenue, Madison, WI 53706–1396, USA.

* To whom correspondence should be addressed. E-mail: yzhao{at}

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