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Science 301 (5637): 1230-1233

Copyright © 2003 by the American Association for the Advancement of Science

Cleavage of Arabidopsis PBS1 by a Bacterial Type III Effector

Feng Shao,1*{dagger} Catherine Golstein,2* Jules Ade,2* Mark Stoutemyer,2 Jack E. Dixon,1{dagger} Roger W. Innes2{ddagger}

Abstract: Plant disease-resistance (R) proteins are thought to function as receptors for ligands produced directly or indirectly by pathogen avirulence (Avr) proteins. The biochemical functions of most Avr proteins are unknown, and the mechanisms by which they activate R proteins have not been determined. In Arabidopsis, resistance to Pseudomonas syringae strains expressing AvrPphB requires RPS5, a member of the class of R proteins that have a predicted nucleotide-binding site and leucine-rich repeats, and PBS1, a protein kinase. AvrPphB was found to proteolytically cleave PBS1, and this cleavage was required for RPS5-mediated resistance, which indicates that AvrPphB is detected indirectly via its enzymatic activity.

1 Department of Biological Chemistry, Medical School and Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, USA. 2 Department of Biology, Indiana University, Bloomington, IN 47405, USA.

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* These authors contributed equally to this work.

{dagger} Present address: Departments of Pharmacology, Cellular and Molecular Medicine, School of Medicine, and Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093, USA.

{ddagger} To whom correspondence should be addressed. E-mail: rinnes{at}

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